ECB-ART-32234
J Cell Sci Suppl
1986 Jan 01;5:197-204. doi: 10.1242/jcs.1986.supplement_5.13.
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Characterization of the sea-urchin egg microtubule-activated ATPase.
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We have found that cytoplasmic extracts from unfertilized sea-urchin eggs contain a prominent microtubule-activated ATPase activity. This activity is induced by polymeric tubulin, but not by tubulin subunits. The activity cosediments with taxol-stabilized microtubules in an ATP-independent manner. We have separated the ATPase from cytoplasmic dynein and other ATPases on sucrose gradients. The sedimentation, enzymic and microtubule-binding properties of the microtubule-activated species show it to be distinct from cytoplasmic dynein, myosin and kinesin. Since the major function of microtubules in the early sea-urchin embryo is in mitosis, this enzyme represents a new candidate for a role in spindle motility.
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Genes referenced: dnah3 LOC100887844 LOC581395 tubgcp2