Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
???displayArticle.abstract???
When Arbacia punctulata spermatozoa are incubated in seawater containing ammonium hydroxide (pH 8.8), the sperm plasma membrane-bound guanylate cyclase is dephosphorylated, its electrophoretic mobility increases (from an apparent molecular mass of 160 to 150 kD), and its enzymatic activity decreases 3.5-fold. Transfer of these cells into ammonium-free seawater (pH 7.4) results in the rephosphorylation of the cyclase, its reconversion to 160 kD, and recovery of the enzymatic activity lost upon dephosphorylation. This is the first direct demonstration that the activity of membrane-bound guanylate cyclase can be regulated by phosphorylation. A plasma membrane preparation is described that specifically supports the in vitro phosphorylation of the guanylate cyclase. This preparation will be useful in more detailed studies on the relationship between phosphorylation state and enzymatic activity of membrane-bound guanylate cyclase.
Adkins,
Two structurally and functionally different forms of the transforming protein of PRC II avian sarcoma virus.
1982, Pubmed
Adkins,
Two structurally and functionally different forms of the transforming protein of PRC II avian sarcoma virus.
1982,
Pubmed
Cleveland,
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
1977,
Pubmed
Dangott,
Identification and partial characterization of the receptor for speract.
1984,
Pubmed
,
Echinobase
de Azeredo,
Light-induced changes in energy metabolites, guanine nucleotides, and guanylate cyclase within frog retinal layers.
1981,
Pubmed
Fenner,
Quantification of Coomassie Blue stained proteins in polyacrylamide gels based on analyses of eluted dye.
1975,
Pubmed
Garbers,
Regulation of sea urchin sperm cyclic AMP-dependent protein kinases by an egg associated factor.
1980,
Pubmed
,
Echinobase
Garbers,
The amino acid sequence and chemical synthesis of speract and of speract analogues.
1982,
Pubmed
,
Echinobase
Garbers,
The regulation of spermatozoa by calcium cyclic nucleotides.
1980,
Pubmed
Garbers,
Guanylate cyclase assay methods.
1979,
Pubmed
Garbers,
Elevation of sperm adenosine 3':5'-monophosphate concentrations by a fucose-sulfate-rich complex associated with eggs: I. Structural characterization.
1983,
Pubmed
,
Echinobase
Goldberg,
Cyclic GMP metabolism and involvement in biological regulation.
1977,
Pubmed
Gray,
Guanylate cyclase of sea urchin sperm: subcellular localization.
1976,
Pubmed
,
Echinobase
Gray,
Enzymes of cyclic nucleotide metabolism in invertebrate and vertebrate sperm.
1976,
Pubmed
,
Echinobase
Hansbrough,
Speract. Purification and characterization of a peptide associated with eggs that activates spermatozoa.
1981,
Pubmed
,
Echinobase
Lee,
An adenosine 3':5' monophosphate dependent protein kinase from sea urchin spermatozoa.
1976,
Pubmed
,
Echinobase
Morrissey,
Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.
1981,
Pubmed
Murad,
Properties and regulation of guanylate cyclase and some proposed functions for cyclic GMP.
1979,
Pubmed
Nikodem,
Protein fingerprinting by SDS-gel electrophoresis after partial fragmentation with CNBr.
1979,
Pubmed
Nomura,
Synthetic study on the structure-activity relationship of sperm activating peptides from the jelly coat of sea urchin eggs.
1985,
Pubmed
,
Echinobase
Ramarao,
Receptor-mediated regulation of guanylate cyclase activity in spermatozoa.
1985,
Pubmed
,
Echinobase
Roach,
Protein kinases.
1984,
Pubmed
Sano,
Subcellular localizations of guanylate cyclase and 3',5'-cyclic nucleotide phosphodiesterase in sea urchin sperm.
1976,
Pubmed
,
Echinobase
Schackmann,
A partial sequence of ionic changes associated with the acrosome reaction of Strongylocentrotus purpuratus.
1981,
Pubmed
,
Echinobase
Schackmann,
Membrane potential depolarization and increased intracellular pH accompany the acrosome reaction of sea urchin sperm.
1981,
Pubmed
,
Echinobase
Schackmann,
The acrosome reaction of Strongylocentrotus purpuratus sperm. Ion requirements and movements.
1978,
Pubmed
,
Echinobase
SeGall,
Chemical characterization of the component of the jelly coat from sea urchin eggs responsible for induction of the acrosome reaction.
1979,
Pubmed
,
Echinobase
SeGall,
Jelly coat and induction of the acrosome reaction in echinoid sperm.
1981,
Pubmed
,
Echinobase
Smith,
Two apparent molecular weight forms of human and monkey phenylalanine hydroxylase are due to phosphorylation.
1984,
Pubmed
Suzuki,
Purification and the primary structure of sperm-activity peptides from the jelly coat of sea urchin eggs.
1981,
Pubmed
,
Echinobase
Suzuki,
A peptide associated with eggs causes a mobility shift in a major plasma membrane protein of spermatozoa.
1984,
Pubmed
,
Echinobase
Suzuki,
Stimulation of sperm respiration rates by speract and resact at alkaline extracellular pH.
1984,
Pubmed
,
Echinobase
Swarup,
Phosphoprotein phosphatase activity of sea urchin spermatozoa.
1982,
Pubmed
,
Echinobase
Tilney,
Polymerization of actin. IV. Role of Ca++ and H+ in the assembly of actin and in membrane fusion in the acrosomal reaction of echinoderm sperm.
1978,
Pubmed
,
Echinobase
Udenfriend,
Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range.
1972,
Pubmed
Waldman,
Atrial natriuretic factor selectively activates particulate guanylate cyclase and elevates cyclic GMP in rat tissues.
1984,
Pubmed
Ward,
Effects of extracellular egg factors on sperm guanylate cyclase.
1985,
Pubmed
,
Echinobase
Ward,
Chemotaxis of Arbacia punctulata spermatozoa to resact, a peptide from the egg jelly layer.
1985,
Pubmed
,
Echinobase
Ward,
Dephosphorylation of a major sperm membrane protein is induced by egg jelly during sea urchin fertilization.
1983,
Pubmed
,
Echinobase
Wegener,
Phosphorylation-induced mobility shift in phospholamban in sodium dodecyl sulfate-polyacrylamide gels. Evidence for a protein structure consisting of multiple identical phosphorylatable subunits.
1984,
Pubmed
Zwiller,
Evidence for phosphorylation of rat brain guanylate cyclase by cyclic AMP-dependent protein kinase.
1981,
Pubmed
Zwiller,
Protein kinase C catalyzes phosphorylation of guanylate cyclase in vitro.
1985,
Pubmed
