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Proc Natl Acad Sci U S A
1985 Feb 01;824:1094-8. doi: 10.1073/pnas.82.4.1094.
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Polyadenylylation of sea urchin histone RNA sequences in transfected COS cells.
Nordstrom JL
,
Hall SL
,
Kessler MM
.
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The region of pSV2neo that encompasses the simian virus 40 early polyadenylylation signal was replaced with a DNA fragment that spans the 3'' end of a sea urchin (Psammechinus miliaris) histone H2A gene. This clone, pMK2.H2A(3''), was used to transfect COS cells. RNA analysis revealed that transcripts from pMK2.H2A(3'') were polyadenylylated at a site 85 nucleotides downstream from the expected 3'' end of mature H2A mRNA. Nucleotide sequencing showed that the site of poly(A) addition was located 10 nucleotides downstream from a cluster of four A-A-U-A-A-A sequences. The lower accumulation of MK2.H2A(3'') mRNA, which was 5-10% that of SV2neo mRNA, suggests that the H2A polyadenylylation signal is relatively inefficient. The relationship of the above findings to the 3'' end processing of other histone mRNAs is discussed.
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