Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Mol Evol
1988 Jan 01;271:36-44. doi: 10.1007/bf02099728.
Show Gene links
Show Anatomy links
Histone genes in three sea star species: cluster arrangement, transcriptional polarity, and analyses of the flanking regions of H3 and H4 genes.
Cool D
,
Banfield D
,
Honda BM
,
Smith MJ
.
???displayArticle.abstract???
The arrangement of core histone genes and their transcriptional polarity has been determined for three species of sea stars (Pisaster ochraceus, P. brevispinus, and Dermasterias imbricata) representing two orders which diverged over 500 million years ago. Each species has approximately 500 core histones cluster repeats per haploid genome. The close phylogenetic relationship between the Pisaster species is evident from the correspondence of restriction sites in the repeat element, identical arrangement of core histones, and high degree of sequence homology in both the coding and spacer regions of the H3 gene. The Dermasterias repeat has the same gene order and transcriptional polarity of core histones, but its restriction map is significantly different. Moreover, the Dermasterias H3 gene has the same amino acid sequence, but in comparison to Pisaster nucleotide sequences, shows a high level of silent substitutions. Analyses of the nucleotide sequence of the 5'' and 3'' regions surrounding the H3 gene from each species demonstrate the presence of appropriately spaced consensus and processing signal segments. The 3'' spacer segment of the Dermasterias H4 gene contains an unusual, threefold tandemly repeated, 21-nucleotide, AT-rich sequence. No similar sequence is seen in the P. brevispinus H4 3'' region, but these two species show a striking regularity of distribution of five different homologous oligomers in the 3'' spacer.
Blin,
A general method for isolation of high molecular weight DNA from eukaryotes.
1976, Pubmed
Blin,
A general method for isolation of high molecular weight DNA from eukaryotes.
1976,
Pubmed
Domier,
Drosophila virilis histone gene clusters lacking H1 coding segments.
1986,
Pubmed
Gasser,
Cohabitation of scaffold binding regions with upstream/enhancer elements of three developmentally regulated genes of D. melanogaster.
1986,
Pubmed
Guo,
Exonuclease III: use for DNA sequence analysis and in specific deletions of nucleotides.
1983,
Pubmed
Hattori,
Dideoxy sequencing method using denatured plasmid templates.
1986,
Pubmed
Henikoff,
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
1984,
Pubmed
Hentschel,
The organization and expression of histone gene families.
1981,
Pubmed
Johnson,
Tripartite sequences within and 3' to the sea urchin H2A histone gene display properties associated with a transcriptional termination process.
1986,
Pubmed
,
Echinobase
Kedes,
Histone messengers and histone genes.
1976,
Pubmed
,
Echinobase
Kovesdi,
Actin genes from the sea star Pisaster ochraceus.
1984,
Pubmed
,
Echinobase
Laskey,
Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
1977,
Pubmed
Maniatis,
The isolation of structural genes from libraries of eucaryotic DNA.
1978,
Pubmed
Maxson,
Distinct organizations and patterns of expression of early and late histone gene sets in the sea urchin.
1983,
Pubmed
,
Echinobase
Messing,
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
1982,
Pubmed
Pearson,
A program for least squares analysis of reassociation and hybridization data.
1977,
Pubmed
Perler,
The evolution of genes: the chicken preproinsulin gene.
1980,
Pubmed
Perry,
Genomic organization and nucleotide sequence of two distinct histone gene clusters from Xenopus laevis. Identification of novel conserved upstream sequence elements.
1985,
Pubmed
Raff,
Origin of a gene regulatory mechanism in the evolution of echinoderms.
1984,
Pubmed
,
Echinobase
Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schaufele,
Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3' end of H3 messenger RNA.
1986,
Pubmed
,
Echinobase
Smith,
Single copy DNA homology in sea stars.
1982,
Pubmed
,
Echinobase
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Wells,
Compilation analysis of histones and histone genes.
1986,
Pubmed
Wells,
Codon usage in histone gene families of higher eukaryotes reflects functional rather than phylogenetic relationships.
1986,
Pubmed
