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Sea urchin early and late H4 histone genes bind a specific transcription factor in a stable preinitiation complex.
Tung L
,
Morris GF
,
Yager LN
,
Weinberg ES
.
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Early embryonic H4 (EH4) and H2B (EH2B) and late embryonic H4 (LH4) histone genes were transcribed in vitro in a nuclear extract from hatching blastula embryos of the sea urchin Strongylocentrotus purpuratus. The extract was prepared by slight modifications of the methods of Morris et al. (G. F. Morris, D. H. Price, and W. F. Marzluff, Proc. Natl. Acad. Sci. USA 83:3674-3678, 1986) that have been used to obtain a cell-free transcription system from embryos of the sea urchin Lytechinus variegatus. Achievement of maximum levels of transcription of the EH4 and LH4 genes required a 5- to 10-min preincubation of template with extract in the absence of ribonucleoside triphosphates. This preincubation allowed the formation of a stable complex which was preferentially transcribed compared with a second EH4 or LH4 template that was added 10 min later. Although the EH4 gene inhibited both EH4 and LH4 gene transcription in this assay and although the LH4 gene inhibited both EH4 and LH4 genes, neither of these genes inhibited transcription of the EH2B gene. Preincubation with the EH2B gene had no effect on the transcription of subsequently added EH4 or LH4 genes. Using this template commitment assay, we showed that the site of binding of at least one essential factor required for transcription of both EH4 and LH4 genes was located between positions -102 and -436 relative to the 5'' terminus of the EH4 mRNA. Moreover, deletion of this region resulted in a reduction in EH4 gene transcription in vitro. The sea urchin gene-specific trans-acting factors, in the analysis of the cis-acting sequences with which they interact, and in biochemical studies on the formation of stable transcription complexes.
Bieker,
Formation of a rate-limiting intermediate in 5S RNA gene transcription.
1985, Pubmed
Bieker,
Formation of a rate-limiting intermediate in 5S RNA gene transcription.
1985,
Pubmed
Birchmeier,
The terminal RNA stem-loop structure and 80 bp of spacer DNA are required for the formation of 3' termini of sea urchin H2A mRNA.
1983,
Pubmed
,
Echinobase
Birchmeier,
Generation of authentic 3' termini of an H2A mRNA in vivo is dependent on a short inverted DNA repeat and on spacer sequences.
1982,
Pubmed
,
Echinobase
Bodner,
A pituitary-specific trans-acting factor can stimulate transcription from the growth hormone promoter in extracts of nonexpressing cells.
1987,
Pubmed
Bogenhagen,
Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
1982,
Pubmed
Brown,
The role of stable complexes that repress and activate eucaryotic genes.
1984,
Pubmed
Busslinger,
Synthesis of sperm and late histone cDNAs of the sea urchin with a primer complementary to the conserved 3' terminal palindrome: evidence for tissue-specific and more general histone gene variants.
1985,
Pubmed
,
Echinobase
Childs,
Histone gene expression during sea urchin embryogenesis: isolation and characterization of early and late messenger RNAs of Strongylocentrotus purpuratus by gene-specific hybridization and template activity.
1979,
Pubmed
,
Echinobase
Childs,
Sea urchin (lytechinus pictus) late-stage histone H3 and H4 genes: characterization and mapping of a clustered but nontandemly linked multigene family.
1982,
Pubmed
,
Echinobase
Cizewski,
A stable transcription complex directs mouse ribosomal RNA synthesis by RNA polymerase I.
1983,
Pubmed
Colin,
Closely linked early and late histone H2B genes are differentially expressed after microinjection into sea urchin zygotes.
1988,
Pubmed
,
Echinobase
Dailey,
Distinct transcription factors bind specifically to two regions of the human histone H4 promoter.
1986,
Pubmed
Davison,
Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequences.
1983,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Fire,
Interactions between RNA polymerase II, factors, and template leading to accurate transcription.
1984,
Pubmed
Flytzanis,
Ontogenic activation of a fusion gene introduced into sea urchin eggs.
1987,
Pubmed
,
Echinobase
Flytzanis,
Persistence and integration of cloned DNA in postembryonic sea urchins.
1985,
Pubmed
,
Echinobase
Franks,
Spatially deranged though temporally correct expression of Strongylocentrotus purpuratus actin gene fusion in transgenic embryos of a different sea urchin family.
1988,
Pubmed
,
Echinobase
Gorski,
Tissue-specific in vitro transcription from the mouse albumin promoter.
1986,
Pubmed
Grunstein,
Hatching in the sea urchin Lytechinus pictus is accompanied by a shift in histone H4 gene activity.
1978,
Pubmed
,
Echinobase
Hanly,
Identification of promoter elements necessary for transcriptional regulation of a human histone H4 gene in vitro.
1985,
Pubmed
Hawley,
Separation and partial characterization of three functional steps in transcription initiation by human RNA polymerase II.
1985,
Pubmed
Hawley,
Functional steps in transcription initiation and reinitiation from the major late promoter in a HeLa nuclear extract.
1987,
Pubmed
Heberlein,
Temporal pattern of alcohol dehydrogenase gene transcription reproduced by Drosophila stage-specific embryonic extracts.
1988,
Pubmed
Heintz,
Transcription of human histone genes in extracts from synchronized HeLa cells.
1984,
Pubmed
Hentschel,
The organization and expression of histone gene families.
1981,
Pubmed
Hieter,
Histone gene switch in the sea urchin embryo. Identification of late embryonic histone messenger ribonucleic acids and the control of their synthesis.
1979,
Pubmed
,
Echinobase
Hough-Evans,
Correct cell-type-specific expression of a fusion gene injected into sea urchin eggs.
1987,
Pubmed
,
Echinobase
Ito,
Synthesis and turnover of late H2B histone mRNA in developing embryos of the sea urchin, Strongylocentrotus purpuratus.
1988,
Pubmed
,
Echinobase
Katula,
Ontogenic expression of a CyI actin fusion gene injected into sea urchin eggs.
1987,
Pubmed
,
Echinobase
Kaumeyer,
Sequence, organization and expression of late embryonic H3 and H4 histone genes from the sea urchin, Strongylocentrotus purpuratus.
1986,
Pubmed
,
Echinobase
Kemler,
Characterization of two nonallelic pairs of late histone H2A and H2B genes of the sea urchin: differential regulation in the embryo and tissue-specific expression in the adult.
1986,
Pubmed
,
Echinobase
Knowles,
Comparison of the late H1 histone genes of the sea urchins Lytechinus pictus and Strongelocentrotus purpuratus.
1986,
Pubmed
,
Echinobase
Knowles,
Isolation, characterization, and expression of the gene encoding the late histone subtype H1-gamma of the sea urchin Strongylocentrotus purpuratus.
1987,
Pubmed
,
Echinobase
Knowles,
Temporal expression of late histone messenger RNA in the sea urchin Lytechinus pictus.
1984,
Pubmed
,
Echinobase
Lai,
Both basal and ontogenic promoter elements affect the timing and level of expression of a sea urchin H1 gene during early embryogenesis.
1988,
Pubmed
,
Echinobase
Lassar,
Transcription of class III genes: formation of preinitiation complexes.
1983,
Pubmed
Manley,
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
1980,
Pubmed
Mattaj,
An enhancer-like sequence within the Xenopus U2 gene promoter facilitates the formation of stable transcription complexes.
1985,
Pubmed
Mauron,
Accumulation of individual histone mRNAs during embryogenesis of the sea urchin Strongylocentrotus purpuratus.
1982,
Pubmed
,
Echinobase
Maxson,
Distinct organizations and patterns of expression of early and late histone gene sets in the sea urchin.
1983,
Pubmed
,
Echinobase
Maxson,
Expression and organization of histone genes.
1983,
Pubmed
,
Echinobase
Maxson,
Accumulation of the early histone messenger RNAs during the development of Strongylocentrotus purpuratus.
1982,
Pubmed
,
Echinobase
Maxson,
The rate of synthesis of histone mRNA during the development of sea urchin embryos (Strongylocentrotus purpuratus).
1981,
Pubmed
,
Echinobase
McConkey,
TFIIIA binds with equal affinity to somatic and major oocyte 5S RNA genes.
1988,
Pubmed
McMahon,
Introduction of cloned DNA into sea urchin egg cytoplasm: replication and persistence during embryogenesis.
1985,
Pubmed
,
Echinobase
Morris,
Synthesis of U1 RNA in a DNA-dependent system from sea urchin embryos.
1986,
Pubmed
,
Echinobase
Morris,
A factor in sea urchin eggs inhibits transcription in isolated nuclei by sea urchin RNA polymerase III.
1983,
Pubmed
,
Echinobase
Overton,
Length and sequence heterogeneity of the histone gene repeat unit of the sea urchin, S. purpuratus.
1978,
Pubmed
,
Echinobase
Perry,
Genomic organization and nucleotide sequence of two distinct histone gene clusters from Xenopus laevis. Identification of novel conserved upstream sequence elements.
1985,
Pubmed
Sollner-Webb,
Transcription of cloned eukaryotic ribosomal RNA genes.
1986,
Pubmed
Sures,
Leader sequences of Strongylocentrotus purpuratus histone mRNAs start at a unique heptanucleotide common to all five histone genes.
1980,
Pubmed
,
Echinobase
Vieira,
Production of single-stranded plasmid DNA.
1987,
Pubmed
Vitelli,
Developmental regulation of micro-injected histone genes in sea urchin embryos.
1988,
Pubmed
,
Echinobase
Wandelt,
Formation of stable preinitiation complexes is a prerequisite for ribosomal DNA transcription in vitro.
1983,
Pubmed
Wasylyk,
Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes.
1980,
Pubmed
Weil,
Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.
1979,
Pubmed
Weinberg,
Timing and rates of synthesis of early histone mRNA in the embryo of Strongylocentrotus purpuratus.
1983,
Pubmed
,
Echinobase
Wolffe,
Developmental regulation of two 5S ribosomal RNA genes.
1988,
Pubmed
Wolffe,
Transcription fraction TFIIIC can regulate differential Xenopus 5S RNA gene transcription in vitro.
1988,
Pubmed
Wolffe,
Differential 5S RNA gene expression in vitro.
1987,
Pubmed
