Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Proc Natl Acad Sci U S A
1989 Dec 01;8623:9303-7. doi: 10.1073/pnas.86.23.9303.
Show Gene links
Show Anatomy links
Structure and developmental expression of a sea urchin fibrillar collagen gene.
D'Alessio M
,
Ramirez F
,
Suzuki HR
,
Solursh M
,
Gambino R
.
???displayArticle.abstract???
We have isolated and characterized cDNA and genomic clones that specify a Paracentrotus lividus procollagen chain. The cDNAs code for 160 uninterrupted Gly-Xaa-Yaa triplets and a 252-amino acid carboxyl propeptide. Analysis of the deduced amino acid sequences indicated that the sea urchin polypeptide exhibits structural features that are characteristic of the fibril-forming class of collagen molecules. Partial characterization of two genomic recombinants revealed that the 3'' end of the echinoid gene displays a complex organization that closely resembles that of a prototypical vertebrate fibrillar collagen gene. In situ and Northern (RNA) blot hybridizations established the size, time of appearance, and tissue distribution of the collagen transcripts in the developing sea urchin embryo. Collagen mRNA, approximately equal to 6 kilobases in size, is first detected in the forming primary mesenchyme cells of late blastulae where it progressively accumulates until the free swimming/feeding pluteus larval stage. Interestingly, collagen transcripts are also detected in the forming secondary mesenchyme cells of late gastrulae, and by the prism stage, their derivatives appear to be the most intensively labeled cells.
Angerer,
Expression of a collagen gene in mesenchyme lineages of the Strongylocentrotus purpuratus embryo.
1988, Pubmed,
Echinobase
Angerer,
Expression of a collagen gene in mesenchyme lineages of the Strongylocentrotus purpuratus embryo.
1988,
Pubmed
,
Echinobase
Benson-Chanda,
Cloning and analysis of the 5' portion of the human type-III procollagen gene (COL3A1).
1989,
Pubmed
Bernard,
Cloning and sequencing of pro-alpha 1 (XI) collagen cDNA demonstrates that type XI belongs to the fibrillar class of collagens and reveals that the expression of the gene is not restricted to cartilagenous tissue.
1988,
Pubmed
Blankenship,
Collagen metabolism and spicule formation in sea urchin micromeres.
1984,
Pubmed
,
Echinobase
Blumberg,
Drosophila basement membrane procollagen alpha 1(IV). II. Complete cDNA sequence, genomic structure, and general implications for supramolecular assemblies.
1988,
Pubmed
Corpet,
Multiple sequence alignment with hierarchical clustering.
1988,
Pubmed
Cox,
Cell lineage-specific programs of expression of multiple actin genes during sea urchin embryogenesis.
1986,
Pubmed
,
Echinobase
Davidson,
Lineage-specific gene expression and the regulative capacities of the sea urchin embryo: a proposed mechanism.
1989,
Pubmed
,
Echinobase
Dion,
COOH-terminal propeptides of the major human procollagens. Structural, functional and genetic comparisons.
1987,
Pubmed
Ettensohn,
Cell lineage conversion in the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Gibson,
The origin of pigment cells in embryos of the sea urchin Strongylocentrotus purpuratus.
1985,
Pubmed
,
Echinobase
Golob,
The onset of collagen synthesis in sea urchin embryos.
1974,
Pubmed
,
Echinobase
Greenburg,
Cytodifferentiation and tissue phenotype change during transformation of embryonic lens epithelium to mesenchyme-like cells in vitro.
1986,
Pubmed
Hattori,
Dideoxy sequencing method using denatured plasmid templates.
1986,
Pubmed
Henikoff,
Unidirectional digestion with exonuclease III in DNA sequence analysis.
1987,
Pubmed
Kramer,
Comparisons of the complete sequences of two collagen genes from Caenorhabditis elegans.
1982,
Pubmed
Nemer,
Sea-urchin RNAs displaying differences in developmental regulation and in complementarity to a collagen exon probe.
1988,
Pubmed
,
Echinobase
Saitta,
Isolation of a putative collagen-like gene from the sea urchin Paracentrotus lividus.
1989,
Pubmed
,
Echinobase
Sucov,
A regulatory domain that directs lineage-specific expression of a skeletal matrix protein gene in the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Swalla,
Analysis of type II collagen RNA localization in chick wing buds by in situ hybridization.
1988,
Pubmed
Venkatesan,
Structure and developmentally regulated expression of a Strongylocentrotus purpuratus collagen gene.
1986,
Pubmed
,
Echinobase
Wessel,
Gastrulation in the sea urchin embryo requires the deposition of crosslinked collagen within the extracellular matrix.
1987,
Pubmed
,
Echinobase
Wessel,
Ontogeny of the basal lamina in the sea urchin embryo.
1984,
Pubmed
,
Echinobase
