Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
A stable alpha-helical element in the carboxy-terminal domain of free and chromatin-bound histone H1 from sea urchin sperm.
Hill CS
,
Martin SR
,
Thomas JO
.
???displayArticle.abstract???
The carboxy-terminal domain (residues 121-248) of sea urchin sperm-specific H1 is not random coil but partly alpha-helical, even in 1 mM sodium phosphate, pH 7. The helix resides in a 57 residue proline-free segment which, in the intact histone, immediately abuts the central globular domain. The proline-free region, which is rich in lysine and alanine, is relatively resistant to tryptic digestion when the carboxy-terminal domain is bound to DNA. Two (overlapping) resistant peptides are shown by circular dichroism measurements to be substantially alpha-helical in 1 mM sodium phosphate and to increase in helix content to approximately 70% in 1 M NaCLO4. Tryptic digestion of chromatin gives resistant fragments containing both the globular domain and the contiguous proline-free segment, strongly suggesting that the alpha-helical segment also exists in chromatin, where it would be ideally placed to direct the path of the linker DNA entering or leaving the nucleosome. The linker in sea urchin sperm chromatin is long (approximately 74 bp), and the unusually long alpha-helical segment in the carboxy-terminal tail of sperm H1 which has amphipathic character due to the alanine distribution, and is likely to be curved, may be a special feature tailored to organize it.
Allan,
Roles of H1 domains in determining higher order chromatin structure and H1 location.
1986, Pubmed
Allan,
Roles of H1 domains in determining higher order chromatin structure and H1 location.
1986,
Pubmed
Allan,
The structure of histone H1 and its location in chromatin.
1980,
Pubmed
Babu,
Three-dimensional structure of calmodulin.
1985,
Pubmed
Baker,
Hydrogen bonding in globular proteins.
1984,
Pubmed
Barlow,
Helix geometry in proteins.
1988,
Pubmed
Bierzynski,
A salt bridge stabilizes the helix formed by isolated C-peptide of RNase A.
1982,
Pubmed
Blundell,
Solvent-induced distortions and the curvature of alpha-helices.
1984,
Pubmed
Carroll,
Optical properties of deoxyribonucleic acid--polylysine complexes.
1972,
Pubmed
Chang,
Conformational studies of nucleoprotein. Circular dichroism of deoxyribonucleic acid base pairs bound by polylysine.
1973,
Pubmed
Chou,
Empirical predictions of protein conformation.
1978,
Pubmed
Chou,
Prediction of protein conformation.
1974,
Pubmed
Clark,
Salt-dependent co-operative interaction of histone H1 with linear DNA.
1986,
Pubmed
Clark,
Differences in the binding of H1 variants to DNA. Cooperativity and linker-length related distribution.
1988,
Pubmed
,
Echinobase
Clark,
Alpha-helix in the carboxy-terminal domains of histones H1 and H5.
1988,
Pubmed
,
Echinobase
Clore,
The polypeptide fold of the globular domain of histone H5 in solution. A study using nuclear magnetic resonance, distance geometry and restrained molecular dynamics.
1987,
Pubmed
Cole,
A minireview of microheterogeneity in H1 histone and its possible significance.
1984,
Pubmed
Garnier,
Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
1978,
Pubmed
Giancotti,
Secondary and tertiary structural differences between histone H1 molecules from calf thymus and sea-urchin (Sphaerechinus granularis) sperm.
1981,
Pubmed
,
Echinobase
Hartman,
Studies on the role and mode of operation of the very-lysine-rich histone H1 in eukaryote chromatin. The three structural regions of the histone H1 molecule.
1977,
Pubmed
Haynes,
Structure of nucleic acid-poly base complexes.
1970,
Pubmed
Herzberg,
Structure of the calcium regulatory muscle protein troponin-C at 2.8 A resolution.
1985,
Pubmed
HOLZWARTH,
THE ULTRAVIOLET CIRCULAR DICHROISM OF POLYPEPTIDES.
1965,
Pubmed
Huey,
Condensed states of nucleic acids. III. psi(+) and psi(-) conformational transitions of DNA induced by ethanol and salt.
1981,
Pubmed
Jordan,
Structure and circular dichroism of DNA in concentrated polymer solutions.
1972,
Pubmed
Lambert,
Lysine-containing DNA-binding regions on the surface of the histone octamer in the nucleosome core particle.
1986,
Pubmed
Liao,
Differences among subfractions of H1 histone in their interactions with linear and superhelical DNA. Circular dichroism.
1981,
Pubmed
Marqusee,
Helix stabilization by Glu-...Lys+ salt bridges in short peptides of de novo design.
1987,
Pubmed
Morán,
Condensation of DNA by the C-terminal domain of histone H1. A circular dichroism study.
1985,
Pubmed
Ong,
Chromatin models. The ionic strength dependence of model histone-DNA interactions: circular dichroism studies of lysine-leucine polypeptide-DNA complexes.
1976,
Pubmed
Pearlstone,
The binding site of skeletal alpha-tropomyosin on troponin-T.
1977,
Pubmed
Pinkston,
Studies on interaction between poly(L-lysine 40, L-alanine 60) and deoxyribonucleic acids.
1974,
Pubmed
Puigdomenech,
The chromatin of sea urchin sperm.
1987,
Pubmed
,
Echinobase
Rifkind,
Helix--coil transition of poly-L-arginine: a comparison with other basic polypeptides.
1969,
Pubmed
Sen,
Higher order structure of chromatin: evidence from photochemically detected linear dichroism.
1986,
Pubmed
,
Echinobase
Shoemaker,
Tests of the helix dipole model for stabilization of alpha-helices.
1987,
Pubmed
Staynov,
Footprinting of linker histones H5 and H1 on the nucleosome.
1988,
Pubmed
Stein,
Generation of different nucleosome spacing periodicities in vitro. Possible origin of cell type specificity.
1988,
Pubmed
,
Echinobase
Stein,
Histone H5 can correctly align randomly arranged nucleosomes in a defined in vitro system.
1983,
Pubmed
Strickland,
The primary structure of histone H1 from sperm of the sea urchin Parechinus angulosus. 2. Sequence of the C-terminal CNBr peptide and the entire primary structure.
1980,
Pubmed
,
Echinobase
Sundaralingam,
Molecular structure of troponin C from chicken skeletal muscle at 3-angstrom resolution.
1985,
Pubmed
Sundaralingam,
Stabilization of the long central helix of troponin C by intrahelical salt bridges between charged amino acid side chains.
1985,
Pubmed
Suzuki,
SPKK, a new nucleic acid-binding unit of protein found in histone.
1989,
Pubmed
,
Echinobase
Thoma,
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.
1979,
Pubmed
Thomas,
The study of histone--histone associations by chemical cross-linking.
1978,
Pubmed
Thomas,
Salt-induced folding of sea urchin sperm chromatin.
1986,
Pubmed
,
Echinobase
Tunis-Schneider,
Circular dichroism spectra of oriented and unoriented deoxyribonucleic acid films--a preliminary study.
1970,
Pubmed
van Helden,
A theoretical consideration of the lysine-rich histones: H1 from a mammal and an echinoderm, H5 from erythrocytes.
1982,
Pubmed
,
Echinobase
Widom,
Higher-order structure of long repeat chromatin.
1985,
Pubmed
,
Echinobase
