ECB-ART-31838
Electrophoresis
1989 Oct 01;1010:714-8. doi: 10.1002/elps.1150101010.
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Multiple immunoblot: a sensitive technique to stain proteins and detect multiple antigens on a single two-dimensional replica.
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A multiple immunoblotting technique was developed to positively identify up to three different antigens on a single nitrocellulose replica of a two-dimensional isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel. Three highly sensitive immunoblot assays were selected, including: horseradish peroxidase/luminescence, alkaline phosphatase, and silver-enhanced immunogold. As a major advantage, the method permits a simultaneous detection of up to three different antigens without eluting the antibody-dye complex between staining of single polypeptides, thus providing a highly accurate identification of closely migrating components. The staining procedure is summarized in a flow chart. In addition to the multiple immunoblot staining, some suggestions are provided for a sensitive protein staining.
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???displayArticle.link??? Electrophoresis
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