ECB-ART-31589
J Cell Biol
1990 Apr 01;1104:1049-53. doi: 10.1083/jcb.110.4.1049.
Phorbol ester treatment stimulates tyrosine phosphorylation of a sea urchin egg cortex protein.
???displayArticle.abstract???
Fertilization of the sea urchin
egg results in the phosphorylation, on tyrosine, of a high molecular weight protein localized in the
egg cortex. In the present study, treatment of unfertilized eggs with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate stimulated tyrosine phosphorylation of the high molecular weight cortical protein to levels three- to fivefold higher than that occurring in response to fertilization. Experiments using agents that inhibit the
egg Na+/H+ exchange system or mimic the fertilization-induced shift in cytoplasmic pHi, suggest a signal transduction pathway in which
protein kinase C activates the
egg Na+/H+ exchange system and the resultant cytoplasmic pHi shift promotes tyrosine phosphorylation of the high molecular weight cortical protein.
???displayArticle.pubmedLink???
2157715
???displayArticle.pmcLink???
PMC2116097
???displayArticle.link???
J Cell Biol
???displayArticle.grants???
[+]
Genes referenced:
LOC100887844
LOC586799
pkcl2
References [+] :
Bertics,
Regulation of the epidermal growth factor receptor by phosphorylation.
1985,
Pubmed
Bertics,
Regulation of the epidermal growth factor receptor by phosphorylation.
1985,
Pubmed
Brandriff,
Development and life cycle of the parthenogenetically activated sea urchin embryo.
1975,
Pubmed
,
Echinobase
Casnellie,
Tumor promoters cause changes in the state of phosphorylation and apparent molecular weight of a tyrosine protein kinase in T lymphocytes.
1986,
Pubmed
Cianciolo,
Inhibition of lymphocyte proliferation by a synthetic peptide homologous to retroviral envelope proteins.
1985,
Pubmed
Ciapa,
Two phases of inositol polyphosphate and diacylglycerol production at fertilisation.
1986,
Pubmed
,
Echinobase
Clapper,
Inositol trisphosphate induces calcium release from nonmitochondrial stores i sea urchin egg homogenates.
1985,
Pubmed
,
Echinobase
Cochet,
C-kinase phosphorylates the epidermal growth factor receptor and reduces its epidermal growth factor-stimulated tyrosine protein kinase activity.
1984,
Pubmed
Crabb,
In vitro reconstitution of exocytosis from plasma membrane and isolated secretory vesicles.
1985,
Pubmed
,
Echinobase
Dasgupta,
Tyrosine protein kinase activity during embryogenesis.
1983,
Pubmed
,
Echinobase
Dubé,
The hierarchy of requirements for an elevated intracellular pH during early development of sea urchin embryos.
1985,
Pubmed
,
Echinobase
Gilmore,
Phorbol ester and diacylglycerol induce protein phosphorylation at tyrosine.
1984,
Pubmed
Gottlieb,
Synthetic peptide corresponding to a conserved domain of the retroviral protein p15E blocks IL-1-mediated signal transduction.
1989,
Pubmed
Gould,
Protein kinase C phosphorylates pp60src at a novel site.
1985,
Pubmed
Grunberger,
Tumor-promoting phorbol ester stimulates tyrosine phosphorylation in U-937 monocytes.
1984,
Pubmed
Hidaka,
Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C.
1984,
Pubmed
Hunter,
A thousand and one protein kinases.
1987,
Pubmed
Keller,
Altered in vitro phosphorylation of specific proteins accompanies fertilization of Strongylocentrotus purpuratus eggs.
1980,
Pubmed
,
Echinobase
Lau,
Tumor promoters and diacylglycerol activate the Na+/H+ antiporter of sea urchin eggs.
1986,
Pubmed
,
Echinobase
Nel,
Phorbol ester induces tyrosine phosphorylation in normal and abnormal human B lymphocytes.
1985,
Pubmed
Payan,
Mechanisms regulating intracellular pH in sea urchin eggs.
1983,
Pubmed
,
Echinobase
Peaucellier,
Protein tyrosine phosphorylation in response to fertilization.
1988,
Pubmed
,
Echinobase
Pelech,
Activation of myelin basic protein kinases during echinoderm oocyte maturation and egg fertilization.
1988,
Pubmed
,
Echinobase
Ribot,
Fertilization results in increased tyrosine phosphorylation of egg proteins.
1984,
Pubmed
,
Echinobase
Shen,
1,2-Diacylglycerols mimic phorbol 12-myristate 13-acetate activation of the sea urchin egg.
1986,
Pubmed
,
Echinobase
Shen,
Protein kinase C from sea urchin eggs.
1989,
Pubmed
,
Echinobase
Shen,
Direct measurement of intracellular pH during metabolic derepression of the sea urchin egg.
1978,
Pubmed
,
Echinobase
Shen,
Intracellular pH and the sodium requirement at fertilisation.
1979,
Pubmed
,
Echinobase
Swann,
Stimulation of the Na/H exchanger of sea urchin eggs by phorbol ester.
1985,
Pubmed
,
Echinobase
Whitaker,
Ionic regulation of egg activation.
1982,
Pubmed
,
Echinobase
