Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Proc Natl Acad Sci U S A
1991 Jun 01;8811:4981-5. doi: 10.1073/pnas.88.11.4981.
Show Gene links
Show Anatomy links
Mitogen-activated Swiss mouse 3T3 RSK kinases I and II are related to pp44mpk from sea star oocytes and participate in the regulation of pp90rsk activity.
???displayArticle.abstract???
Using a recombinant rsk gene product as a substrate for in vitro kinase assays, we have identified two mitogen-activated Swiss 3T3 RSK protein kinase activities (referred to as RSK kinase I and RSK kinase II, based on their order of elution from phenyl-Sepharose). Polyclonal antisera prepared against maturation-regulated 44-kDa myelin basic protein (MBP) kinase (pp44mpk) purified from sea star oocytes demonstrated immunocrossreactivity with polypeptides of approximately 44 kDa in the RSK kinase I preparation and approximately 42 kDa in the RSK kinase II preparation, respectively. These polypeptides were also recognized by anti-phosphotyrosine antibodies, and either phosphatase 1B or 2A (tyrosine- and serine/threonine-specific phosphatases, respectively) separately inactivated RSK phosphotransferase activity supporting the notion that tyrosine and serine/threonine phosphorylation are required for activity. In vitro, both RSK kinases and MBP kinase phosphorylated recombinant RSK and generated nearly identical two-dimensional tryptic phosphopeptide maps. They also phosphorylated MBP and microtubule-associated protein 2 but not 40S ribosomal protein S6. Furthermore, these protein kinases phosphorylated and partially activated pp90rsk in immune complexes obtained from quiescent cells.
Ahn,
Evidence for an epidermal growth factor-stimulated protein kinase cascade in Swiss 3T3 cells. Activation of serine peptide kinase activity by myelin basic protein kinases in vitro.
1990, Pubmed
Ahn,
Evidence for an epidermal growth factor-stimulated protein kinase cascade in Swiss 3T3 cells. Activation of serine peptide kinase activity by myelin basic protein kinases in vitro.
1990,
Pubmed
Alcorta,
Sequence and expression of chicken and mouse rsk: homologs of Xenopus laevis ribosomal S6 kinase.
1989,
Pubmed
Anderson,
Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase.
1990,
Pubmed
Banerjee,
Molecular structure of a major insulin/mitogen-activated 70-kDa S6 protein kinase.
1990,
Pubmed
Blenis,
Identification of a ribosomal protein S6 kinase regulated by transformation and growth-promoting stimuli.
1987,
Pubmed
Boulton,
An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control.
1990,
Pubmed
Chen,
Identification of Xenopus S6 protein kinase homologs (pp90rsk) in somatic cells: phosphorylation and activation during initiation of cell proliferation.
1990,
Pubmed
Chen,
Regulation of pp90rsk phosphorylation and S6 phosphotransferase activity in Swiss 3T3 cells by growth factor-, phorbol ester-, and cyclic AMP-mediated signal transduction.
1991,
Pubmed
Chung,
Coordinate regulation of pp90rsk and a distinct protein-serine/threonine kinase activity that phosphorylates recombinant pp90rsk in vitro.
1991,
Pubmed
Cicirelli,
Activation of multiple protein kinases during the burst in protein phosphorylation that precedes the first meiotic cell division in Xenopus oocytes.
1988,
Pubmed
Cooper,
Detection and quantification of phosphotyrosine in proteins.
1983,
Pubmed
Cooper,
Similar effects of platelet-derived growth factor and epidermal growth factor on the phosphorylation of tyrosine in cellular proteins.
1982,
Pubmed
Cooper,
Diverse mitogenic agents induce the phosphorylation of two related 42,000-dalton proteins on tyrosine in quiescent chick cells.
1984,
Pubmed
Ely,
A 42-kD tyrosine kinase substrate linked to chromaffin cell secretion exhibits an associated MAP kinase activity and is highly related to a 42-kD mitogen-stimulated protein in fibroblasts.
1990,
Pubmed
Erikson,
Biochemical characterization of the p34cdc2 protein kinase component of purified maturation-promoting factor from Xenopus eggs.
1989,
Pubmed
Erikson,
Substrate specificity of ribosomal protein S6 kinase II from Xenopus eggs.
1988,
Pubmed
Erikson,
Structure, expression, and regulation of protein kinases involved in the phosphorylation of ribosomal protein S6.
1991,
Pubmed
Fiol,
Formation of protein kinase recognition sites by covalent modification of the substrate. Molecular mechanism for the synergistic action of casein kinase II and glycogen synthase kinase 3.
1987,
Pubmed
Flotow,
Phosphate groups as substrate determinants for casein kinase I action.
1990,
Pubmed
Gilmore,
Phorbol ester and diacylglycerol induce protein phosphorylation at tyrosine.
1984,
Pubmed
Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed
Jones,
A Xenopus ribosomal protein S6 kinase has two apparent kinase domains that are each similar to distinct protein kinases.
1988,
Pubmed
Kozma,
Cloning of the mitogen-activated S6 kinase from rat liver reveals an enzyme of the second messenger subfamily.
1990,
Pubmed
Maller,
Xenopus oocytes and the biochemistry of cell division.
1990,
Pubmed
Nakamura,
Tyrosine phosphorylation of specific proteins after mitogen stimulation of chicken embryo fibroblasts.
1983,
Pubmed
Pelech,
Protein kinase cascades in meiotic and mitotic cell cycle control.
1990,
Pubmed
,
Echinobase
Pelech,
Activation of myelin basic protein kinases during echinoderm oocyte maturation and egg fertilization.
1988,
Pubmed
,
Echinobase
Ray,
Insulin-stimulated microtubule-associated protein kinase is phosphorylated on tyrosine and threonine in vivo.
1988,
Pubmed
Ray,
Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.
1987,
Pubmed
Ray,
Characterization of insulin-stimulated microtubule-associated protein kinase. Rapid isolation and stabilization of a novel serine/threonine kinase from 3T3-L1 cells.
1988,
Pubmed
Rossomando,
Evidence that pp42, a major tyrosine kinase target protein, is a mitogen-activated serine/threonine protein kinase.
1989,
Pubmed
Sanghera,
Role of protein phosphorylation in the maturation-induced activation of a myelin basic protein kinase from sea star oocytes.
1991,
Pubmed
,
Echinobase
Sanghera,
Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.
1990,
Pubmed
,
Echinobase
Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
Sweet,
Two distinct enzymes contribute to biphasic S6 phosphorylation in serum-stimulated chicken embryo fibroblasts.
1990,
Pubmed
Sweet,
Identification of mitogen-responsive ribosomal protein S6 kinase pp90rsk, a homolog of Xenopus S6 kinase II, in chicken embryo fibroblasts.
1990,
Pubmed
