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EMBO J
1991 Feb 01;102:459-66. doi: 10.1002/j.1460-2075.1991.tb07968.x.
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EAP, a highly conserved cellular protein associated with Epstein-Barr virus small RNAs (EBERs).
Toczyski DP
,
Steitz JA
.
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Human B lymphocytes latently infected with Epstein-Barr virus (EBV) synthesize very large amounts (5 x 10(6)/cell) of two small nuclear RNAs called EBERs (Epstein-Barr encoded RNAs). These RNAs are of unknown function and, like many RNA polymerase III (Pol III) transcripts, bind the La autoantigen. We have discovered that the EBERs also associate with a second highly abundant host-encoded protein designated EAP (EBER associated protein). Human EAP is a small (14,777 dalton, 128 amino acid) polypeptide that binds both EBER 1 and EBER 2. EAP is also found in association with one or both of two analogous virally-encoded RNAs found in baboon cells infected with herpesvirus papio (HVP). We have devised a purification procedure for EAP and have cloned its cDNA from a human placental cDNA library using amino acid sequence data and the polymerase chain reaction (PCR). The predicted amino acid sequence of EAP shows a strong resemblance (77% identity) to an endodermal, developmentally regulated sea urchin protein called 217 (Dolecki et al., 1988). EAP contains a potential nuclear localization signal and a highly acidic carboxy terminus, but does not display marked similarity to any other RNA binding proteins.
Adam,
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
1986, Pubmed
Adam,
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
1986,
Pubmed
Aota,
Codon usage tabulated from the GenBank Genetic Sequence Data.
1988,
Pubmed
Bhat,
Construction and analysis of additional adenovirus substitution mutants confirm the complementation of VAI RNA function by two small RNAs encoded by Epstein-Barr virus.
1985,
Pubmed
Chambers,
Genomic structure and amino acid sequence domains of the human La autoantigen.
1988,
Pubmed
Deng,
Nonhistone nuclear antigens reactive with autoantibodies. Immunofluorescence studies on distribution in synchronized cells.
1981,
Pubmed
Dolecki,
A gene expressed in the endoderm of the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Fennewald,
Nucleotide sequence of an mRNA transcribed in latent growth-transforming virus infection indicates that it may encode a membrane protein.
1984,
Pubmed
Francoeur,
Interaction between VA RNA and the lupus antigen La: formation of a ribonucleoprotein particle in vitro.
1982,
Pubmed
Fregien,
Molecular cloning of five individual stage- and tissue-specific mRNA sequences from sea urchin pluteus embryos.
1983,
Pubmed
,
Echinobase
Gottlieb,
The RNA binding protein La influences both the accuracy and the efficiency of RNA polymerase III transcription in vitro.
1989,
Pubmed
Hendrick,
Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.
1981,
Pubmed
Howe,
Epstein-Barr virus small RNA (EBER) genes: unique transcription units that combine RNA polymerase II and III promoter elements.
1989,
Pubmed
Howe,
Localization of Epstein-Barr virus-encoded small RNAs by in situ hybridization.
1986,
Pubmed
Jat,
In vitro transcription of two Epstein-Barr virus specified small RNA molecules.
1982,
Pubmed
Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed
Katze,
Adenovirus VAI RNA complexes with the 68 000 Mr protein kinase to regulate its autophosphorylation and activity.
1987,
Pubmed
Kitajewski,
Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF-2 alpha kinase.
1986,
Pubmed
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Kurilla,
The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodies.
1983,
Pubmed
Lazinski,
Sequence-specific recognition of RNA hairpins by bacteriophage antiterminators requires a conserved arginine-rich motif.
1989,
Pubmed
Lee,
Four novel U RNAs are encoded by a herpesvirus.
1988,
Pubmed
Lerner,
Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus.
1981,
Pubmed
Lerner,
Two novel classes of small ribonucleoproteins detected by antibodies associated with lupus erythematosus.
1981,
Pubmed
Longnecker,
A second Epstein-Barr virus membrane protein (LMP2) is expressed in latent infection and colocalizes with LMP1.
1990,
Pubmed
Marschalek,
CMER, an RNA encoded by human cytomegalovirus is most likely transcribed by RNA polymerase III.
1989,
Pubmed
Matsudaira,
Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
1987,
Pubmed
Mattioli,
Heterogeneity of RNA protein antigens reactive with sera of patients with systemic lupus erythematosus. Description of a cytoplasmic nonribosomal antigen.
1974,
Pubmed
Mellits,
Interaction of adenovirus VA RNAl with the protein kinase DAI: nonequivalence of binding and function.
1990,
Pubmed
Merril,
Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.
1981,
Pubmed
Milburn,
Cloning and expression of eukaryotic initiation factor 4B cDNA: sequence determination identifies a common RNA recognition motif.
1990,
Pubmed
Moreland,
Amino acid sequences that determine the nuclear localization of yeast histone 2B.
1987,
Pubmed
O'Malley,
A mechanism for the control of protein synthesis by adenovirus VA RNAI.
1986,
Pubmed
Pizer,
Characterization of a phosphoprotein associated with the SS-B/La nuclear antigen in adenovirus-infected and uninfected KB cells.
1983,
Pubmed
Provost,
Subsets in systemic lupus erythematosus.
1979,
Pubmed
Reich,
RNA of low molecular weight in KB cells infected with adenovirus type 2.
1966,
Pubmed
Reichel,
Regulation of a protein synthesis initiation factor by adenovirus virus-associated RNA.
1985,
Pubmed
Rinke,
Precursor molecules of both human 5S ribosomal RNA and transfer RNAs are bound by a cellular protein reactive with anti-La lupus antibodies.
1982,
Pubmed
Rosa,
Striking similarities are exhibited by two small Epstein-Barr virus-encoded ribonucleic acids and the adenovirus-associated ribonucleic acids VAI and VAII.
1981,
Pubmed
Schneider,
Adenovirus VAI RNA facilitates the initiation of translation in virus-infected cells.
1984,
Pubmed
Siekierka,
Translational control by adenovirus: lack of virus-associated RNAI during adenovirus infection results in phosphorylation of initiation factor eIF-2 and inhibition of protein synthesis.
1985,
Pubmed
Smith,
Monoclonal antibodies to the Sjögren's syndrome associated antigen SS-B (La).
1985,
Pubmed
Stefano,
Purified lupus antigen La recognizes an oligouridylate stretch common to the 3' termini of RNA polymerase III transcripts.
1984,
Pubmed
Söderlund,
A new species of virus-coded low molecular weight RNA from cells infected with adenovirus type 2.
1976,
Pubmed
Thimmappaya,
Adenovirus VAI RNA is required for efficient translation of viral mRNAs at late times after infection.
1982,
Pubmed
Walker,
A novel continuous sequence of 41 aspartic and glutamic residues in a non-histone chromosomal protein.
1978,
Pubmed
Wassarman,
Nucleotide sequence of HSUR 5 RNA from herpesvirus saimiri.
1989,
Pubmed
Watson,
Amino acid sequence homologies between the high-mobility-group proteins, HMG-T from trout testis and HMG-1 and -2 from calf thymus: is the poly-aspartic-glutamic acid polypeptide within the main chain?
1981,
Pubmed
van Eekelen,
Detection of a cellular polypeptide associated with adenovirus-coded VA RNA using in vitro labeling of proteins cross-linked to RNA.
1982,
Pubmed
van Santen,
Epstein-Barr virus RNA VII: size and direction of transcription of virus-specified cytoplasmic RNAs in a transformed cell line.
1981,
Pubmed