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J Cell Biol
1991 May 01;1134:817-33. doi: 10.1083/jcb.113.4.817.
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Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.
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Kinesin was previously immunolocalized to mitotic apparatuses (MAs) of early sea urchin blastomeres (Scholey, J.M., M.E. Porter, P.M. Grissom, and J.R. McIntosh. 1985. Nature [Lond.]. 318:483-486). Here we report evidence that this MA-associated motor protein is a conventional membrane-bound kinesin, rather than a kinesin-like protein. Our evidence includes the observation that the deduced amino acid sequence of this sea urchin kinesin heavy chain is characteristic of a conventional kinesin. In addition, immunolocalizations using antibodies that distinguish kinesin from kinesin-like proteins confirm that conventional kinesin is concentrated in MAs. Finally, our immunocytochemical data further suggest that conventional kinesin is associated with membranes which accumulate in MAs and interphase asters of early sea urchin embryos, and with vesicles that are distributed in the perinuclear region of coelomocytes. Thus kinesin may function as a microtubule-based vesicle motor in some MAs, as well as in the interphase cytoplasm.
Bloom,
Native structure and physical properties of bovine brain kinesin and identification of the ATP-binding subunit polypeptide.
1988, Pubmed
Bloom,
Native structure and physical properties of bovine brain kinesin and identification of the ATP-binding subunit polypeptide.
1988,
Pubmed
Bomsel,
Microtubule- and motor-dependent fusion in vitro between apical and basolateral endocytic vesicles from MDCK cells.
1990,
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Brady,
A monoclonal antibody against kinesin inhibits both anterograde and retrograde fast axonal transport in squid axoplasm.
1990,
Pubmed
Cohn,
Quantitative analysis of sea urchin egg kinesin-driven microtubule motility.
1989,
Pubmed
,
Echinobase
Dever,
GTP-binding domain: three consensus sequence elements with distinct spacing.
1987,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Edds,
Dynamic aspects of filopodial formation by reorganization of microfilaments.
1977,
Pubmed
,
Echinobase
Endow,
Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin.
1990,
Pubmed
Enos,
Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans.
1990,
Pubmed
Frohman,
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
1988,
Pubmed
Gyllensten,
Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.
1988,
Pubmed
Hackney,
Kinesin ATPase: rate-limiting ADP release.
1988,
Pubmed
Hagan,
Novel potential mitotic motor protein encoded by the fission yeast cut7+ gene.
1990,
Pubmed
Harris,
The role of membranes in the ogranization of the mitotic apparatus.
1975,
Pubmed
,
Echinobase
HARRIS,
Some structural and functional aspects of the mitotic apparatus in sea urchin embryos.
1962,
Pubmed
,
Echinobase
Henson,
Differentiation of a calsequestrin-containing endoplasmic reticulum during sea urchin oogenesis.
1990,
Pubmed
,
Echinobase
Henson,
A calsequestrin-like protein in the endoplasmic reticulum of the sea urchin: localization and dynamics in the egg and first cell cycle embryo.
1989,
Pubmed
,
Echinobase
Hirokawa,
Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration.
1989,
Pubmed
Hollenbeck,
Radial extension of macrophage tubular lysosomes supported by kinesin.
1990,
Pubmed
Hollenbeck,
The distribution, abundance and subcellular localization of kinesin.
1989,
Pubmed
Ingold,
Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
1988,
Pubmed
,
Echinobase
Johnson,
Light chains of sea urchin kinesin identified by immunoadsorption.
1990,
Pubmed
,
Echinobase
Kelly,
Microtubules, membrane traffic, and cell organization.
1990,
Pubmed
Kosik,
The primary structure and analysis of the squid kinesin heavy chain.
1990,
Pubmed
Kuznetsov,
Isolation of a 45-kDa fragment from the kinesin heavy chain with enhanced ATPase and microtubule-binding activities.
1989,
Pubmed
Leslie,
Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles.
1987,
Pubmed
,
Echinobase
McDonald,
Identification and characterization of a gene encoding a kinesin-like protein in Drosophila.
1990,
Pubmed
Meluh,
KAR3, a kinesin-related gene required for yeast nuclear fusion.
1990,
Pubmed
Mierendorf,
Gene isolation by screening lambda gt11 libraries with antibodies.
1987,
Pubmed
Obar,
Molecular cloning of the microtubule-associated mechanochemical enzyme dynamin reveals homology with a new family of GTP-binding proteins.
1990,
Pubmed
Olmsted,
Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
1981,
Pubmed
Petzelt,
Visualization of the Ca-transport system of the mitotic apparatus of sea urchin eggs with a monoclonal antibody.
1986,
Pubmed
,
Echinobase
Pfister,
Monoclonal antibodies to kinesin heavy and light chains stain vesicle-like structures, but not microtubules, in cultured cells.
1989,
Pubmed
Porter,
Characterization of the microtubule movement produced by sea urchin egg kinesin.
1987,
Pubmed
,
Echinobase
REBHUN,
Aster-associated particles in the cleavage of marine invertebrate eggs.
1960,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Saxton,
Kinesin heavy chain is essential for viability and neuromuscular functions in Drosophila, but mutants show no defects in mitosis.
1991,
Pubmed
Scholey,
Identification of kinesin in sea urchin eggs, and evidence for its localization in the mitotic spindle.
1986,
Pubmed
,
Echinobase
Scholey,
Identification of globular mechanochemical heads of kinesin.
1989,
Pubmed
Schroer,
The role of kinesin and other soluble factors in organelle movement along microtubules.
1988,
Pubmed
Sheetz,
What are the functions of kinesin?
1987,
Pubmed
Studier,
Use of T7 RNA polymerase to direct expression of cloned genes.
1990,
Pubmed
Vale,
One motor, many tails: an expanding repertoire of force-generating enzymes.
1990,
Pubmed
Vale,
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.
1985,
Pubmed
Vale,
Intracellular transport using microtubule-based motors.
1987,
Pubmed
Vale,
Different axoplasmic proteins generate movement in opposite directions along microtubules in vitro.
1985,
Pubmed
Vallee,
Potential roles of microtubule-associated motor molecules in cell division.
1990,
Pubmed
Yang,
Isolation and characterization of the gene encoding the heavy chain of Drosophila kinesin.
1988,
Pubmed
Yang,
A three-domain structure of kinesin heavy chain revealed by DNA sequence and microtubule binding analyses.
1989,
Pubmed
Yang,
Evidence that the head of kinesin is sufficient for force generation and motility in vitro.
1990,
Pubmed
,
Echinobase
Zhang,
A kinesin-like protein required for distributive chromosome segregation in Drosophila.
1990,
Pubmed
