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J Cell Biol
1991 Mar 01;1126:1189-97. doi: 10.1083/jcb.112.6.1189.
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Nucleotide specificity of the enzymatic and motile activities of dynein, kinesin, and heavy meromyosin.
Shimizu T
,
Furusawa K
,
Ohashi S
,
Toyoshima YY
,
Okuno M
,
Malik F
,
Vale RD
.
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The substrate specificities of dynein, kinesin, and myosin substrate turnover activity and cytoskeletal filament-driven translocation were examined using 15 ATP analogues. The dyneins were more selective in their substrate utilization than bovine brain kinesin or muscle heavy meromyosin, and even different types of dyneins, such as 14S and 22S dynein from Tetrahymena cilia and the beta-heavy chain-containing particle from the outer-arm dynein of sea urchin flagella, could be distinguished by their substrate specificities. Although bovine brain kinesin and muscle heavy meromyosin both exhibited broad substrate specificities, kinesin-induced microtubule translocation varied over a 50-fold range in speed among the various substrates, whereas heavy meromyosin-induced actin translocation varied only by fourfold. With both kinesin and heavy meromyosin, the relative velocities of filament translocation did not correlate well with the relative filament-activated substrate turnover rates. Furthermore, some ATP analogues that did not support the filament translocation exhibited filament-activated substrate turnover rates. Filament-activated substrate turnover and power production, therefore, appear to become uncoupled with certain substrates. In conclusion, the substrate specificities and coupling to motility are distinct for different types of molecular motor proteins. Such nucleotide "fingerprints" of enzymatic activities of motor proteins may prove useful as a tool for identifying what type of motor is involved in powering a motility-related event that can be reconstituted in vitro.
Cande,
In vitro reactivation of anaphase spindle elongation using isolated diatom spindles.
1985, Pubmed
Cande,
In vitro reactivation of anaphase spindle elongation using isolated diatom spindles.
1985,
Pubmed
Cohn,
Quantitative analysis of sea urchin egg kinesin-driven microtubule motility.
1989,
Pubmed
,
Echinobase
Collins,
Preparation of microtubules from rat liver and testis: cytoplasmic dynein is a major microtubule associated protein.
1989,
Pubmed
Eckstein,
Synthesis and properties of diastereoisomers of adenosine 5'-(O-1-thiotriphosphate) and adenosine 5'-(O-2-thiotriphosphate).
1976,
Pubmed
Gibbons,
Studies on the adenosine triphosphatase activity of 14 S and 30 S dynein from cilia of Tetrahymena.
1966,
Pubmed
Gibbons,
Dynein: A Protein with Adenosine Triphosphatase Activity from Cilia.
1965,
Pubmed
Gibbons,
Dynein ATPases as microtubule motors.
1988,
Pubmed
Gilbert,
A squid dynein isoform promotes axoplasmic vesicle translocation.
1989,
Pubmed
Goodenough,
Motile detergent-extracted cells of Tetrahymena and Chlamydomonas.
1983,
Pubmed
Hackney,
Kinesin ATPase: rate-limiting ADP release.
1988,
Pubmed
Hibberd,
Relationships between chemical and mechanical events during muscular contraction.
1986,
Pubmed
Hirokawa,
Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration.
1989,
Pubmed
Holzbaur,
Microtubules accelerate ADP release by dynein.
1989,
Pubmed
Inaba,
Anthraniloyl ATP, a fluorescent analog of ATP, as a substrate for dynein ATPase and flagellar motility.
1989,
Pubmed
,
Echinobase
Johnson,
Structure and molecular weight of the dynein ATPase.
1983,
Pubmed
Johnson,
Pathway of the microtubule-dynein ATPase and the structure of dynein: a comparison with actomyosin.
1985,
Pubmed
Kiehart,
Molecular genetic dissection of myosin heavy chain function.
1990,
Pubmed
Kuznetsov,
Bovine brain kinesin is a microtubule-activated ATPase.
1986,
Pubmed
Lowey,
Substructure of the myosin molecule. I. Subfragments of myosin by enzymic degradation.
1969,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Marchese-Ragona,
Structure and mass analysis of 14S dynein obtained from Tetrahymena cilia.
1988,
Pubmed
Masuda,
In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells.
1990,
Pubmed
Ogawa,
Studies on flagellar ATPase from sea urchin spermatozoa. I. Purification and some properties of the enzyme.
1972,
Pubmed
,
Echinobase
Okamoto,
A streamlined method of subfragment one preparation from myosin.
1985,
Pubmed
Omoto,
ATP analogs substituted on the 2-position as substrates for dynein ATPase activity.
1989,
Pubmed
Paschal,
Retrograde transport by the microtubule-associated protein MAP 1C.
1987,
Pubmed
Pfister,
Labeling of Chlamydomonas 18 S dynein polypeptides by 8-azidoadenosine 5'-triphosphate, a photoaffinity analog of ATP.
1985,
Pubmed
Pfister,
The photoaffinity probe 8-azidoadenosine 5'-triphosphate selectively labels the heavy chain of Chlamydomonas 12 S dynein.
1984,
Pubmed
Porter,
Characterization of the microtubule movement produced by sea urchin egg kinesin.
1987,
Pubmed
,
Echinobase
Porter,
Characterization of the ATP-sensitive binding of Tetrahymena 30 S dynein to bovine brain microtubules.
1983,
Pubmed
Pratt,
Homology of egg and flagellar dynein. Comparison of ATP-binding sites and primary structure.
1986,
Pubmed
,
Echinobase
Sale,
Isolated beta-heavy chain subunit of dynein translocates microtubules in vitro.
1988,
Pubmed
,
Echinobase
Schliwa,
Nucleotide specificities of anterograde and retrograde organelle transport in Reticulomyxa are indistinguishable.
1991,
Pubmed
Schroer,
Cytoplasmic dynein is a minus end-directed motor for membranous organelles.
1989,
Pubmed
Shimizu,
Adenosine 5'-O-(3-thiotriphosphate) hydrolysis by dynein.
1989,
Pubmed
Shimizu,
Phosphorothioate analogs of ATP as the substrates of dynein and ciliary or flagellar movement.
1990,
Pubmed
,
Echinobase
Shimizu,
Steady-state kinetic study of vanadate-induced inhibition of ciliary dynein adenosinetriphosphatase activity from Tetrahymena.
1981,
Pubmed
Shimizu,
The substrate specificity of dynein from Tetrahymena cilia.
1987,
Pubmed
Shpetner,
Identification of dynamin, a novel mechanochemical enzyme that mediates interactions between microtubules.
1989,
Pubmed
Shpetner,
Characterization of the microtubule-activated ATPase of brain cytoplasmic dynein (MAP 1C).
1988,
Pubmed
Spudich,
The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.
1971,
Pubmed
Takenaka,
Interaction between actomyosin and 8-substituted ATP analogs.
1978,
Pubmed
Tonomura,
Interaction between synthetic ATP analogues and actomyosin systems. IV.
1967,
Pubmed
Toyoshima,
Myosin subfragment-1 is sufficient to move actin filaments in vitro.
1987,
Pubmed
Toyoshima,
Chymotryptic digestion of Tetrahymena 22S dynein. I. Decomposition of three-headed 22S dynein to one- and two-headed particles.
1987,
Pubmed
Vale,
One motor, many tails: an expanding repertoire of force-generating enzymes.
1990,
Pubmed
Vale,
Microtubule translocation properties of intact and proteolytically digested dyneins from Tetrahymena cilia.
1989,
Pubmed
Vale,
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.
1985,
Pubmed
Vale,
One-dimensional diffusion of microtubules bound to flagellar dynein.
1989,
Pubmed
,
Echinobase
Walker,
The Drosophila claret segregation protein is a minus-end directed motor molecule.
1990,
Pubmed
