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Casein kinase II (CKII) is one of several protein kinases that become activated before germinal-vesicle breakdown in maturing sea-star oocytes. Echinoderm CKII was purified over 11,000-fold with a recovery of approximately 10% by sequential fractionation of the oocyte cytosol on tyrosine-agarose, heparin-agarose, casein-agarose and MonoQ. The purified enzyme contained 45, 38 and 28 kDa polypeptides, which corresponded to its alpha, alpha'' and beta subunits respectively. The beta-subunit was autophosphorylated on one major tryptic peptide on serine residues, whereas the alpha''-subunit incorporated phosphate into at least two tryptic peptides primarily on threonine residues. Western-blotting analysis of sea-star oocyte extracts with two different anti-peptide antibodies that recognized conserved regions of the alpha-subunit indicated that the protein levels of the alpha- and alpha''-subunits of CKII were unchanged during oocyte maturation. The purified CKII was partly inactivated (by 25%) by preincubation with protein-serine/threonine phosphatase 2A, but protein-tyrosine phosphatases had no effect. The beta-subunit of CKII was phosphorylated on a serine residue(s) up to 0.54 mol of P/mol of beta-subunit by purified protein kinase C, and this correlated with a 1.5-fold enhancement of its phosphotransferase activity with phosvitin as a substrate. CKII was not a substrate for the maturation-activated myelin basic protein kinase p44mpk from sea-star oocytes, nor for cyclic-AMP-dependent protein kinase. These studies point to possible regulation of CKII by protein phosphorylation.
Ackerman,
Regulation of casein kinase II activity by epidermal growth factor in human A-431 carcinoma cells.
1989, Pubmed
Ackerman,
Regulation of casein kinase II activity by epidermal growth factor in human A-431 carcinoma cells.
1989,
Pubmed Ackerman,
Stimulation of casein kinase II by epidermal growth factor: relationship between the physiological activity of the kinase and the phosphorylation state of its beta subunit.
1990,
Pubmed Agostinis,
Regulation of casein kinase 2 by phosphorylation/dephosphorylation.
1987,
Pubmed Allen,
Isolation and characterization of the calcium- and phospholipid-dependent protein kinase (protein kinase C) subtypes from bovine heart.
1991,
Pubmed Barbosa,
The region of the HPV E7 oncoprotein homologous to adenovirus E1a and Sv40 large T antigen contains separate domains for Rb binding and casein kinase II phosphorylation.
1990,
Pubmed Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed Carroll,
Serum-stimulated cell growth causes oscillations in casein kinase II activity.
1989,
Pubmed Carroll,
Regulating cell growth: casein-kinase-II-dependent phosphorylation of nuclear oncoproteins.
1988,
Pubmed Chen-Wu,
Isolation, sequencing, and disruption of the CKA1 gene encoding the alpha subunit of yeast casein kinase II.
1988,
Pubmed Damuni,
Purification and properties of a protamine kinase and a type II casein kinase from bovine kidney mitochondria.
1988,
Pubmed Delpech,
Rat liver nuclear protein kinases NI and NII. Purification, subunit composition, substrate specificity, possible levels of regulation.
1986,
Pubmed Firzlaff,
The E7 protein of human papillomavirus type 16 is phosphorylated by casein kinase II.
1989,
Pubmed Glover,
Purification and characterization of a type II casein kinase from Drosophila melanogaster.
1983,
Pubmed Gounaris,
Soluble cAMP-independent protein kinase from human spleen.
1987,
Pubmed Grässer,
In vitro phosphorylation of SV40 large T antigen.
1988,
Pubmed Hollmann,
Purification and characterization of a 3'-phosphoadenylylsulfate:chondroitin 6-sulfotransferase from arterial tissue.
1986,
Pubmed Hu,
Expression of wild-type and mutated forms of the catalytic (alpha) subunit of Caenorhabditis elegans casein kinase II in Escherichia coli.
1990,
Pubmed Jakobi,
Human phosvitin/casein kinase type II. Molecular cloning and sequencing of full-length cDNA encoding subunit beta.
1989,
Pubmed Kandror,
Casein kinase II from Rana temporaria oocytes. Intracellular localization and activity during progesterone-induced maturation.
1989,
Pubmed Klarlund,
Insulin-like growth factor I and insulin rapidly increase casein kinase II activity in BALB/c 3T3 fibroblasts.
1988,
Pubmed Krebs,
Casein kinase II as a potentially important enzyme concerned with signal transduction.
1988,
Pubmed Kuenzel,
Substrate specificity determinants for casein kinase II as deduced from studies with synthetic peptides.
1987,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Lin,
Characterization of the catalytic subunit of casein kinase II expressed in Escherichia coli and regulation of activity.
1991,
Pubmed Litchfield,
Synthetic phosphopeptides are substrates for casein kinase II.
1990,
Pubmed Litchfield,
Phosphorylation of the beta subunit of casein kinase II in human A431 cells. Identification of the autophosphorylation site and a site phosphorylated by p34cdc2.
1991,
Pubmed Lüscher,
Myb DNA binding inhibited by phosphorylation at a site deleted during oncogenic activation.
1990,
Pubmed Lüscher,
Myc oncoproteins are phosphorylated by casein kinase II.
1989,
Pubmed Marchiori,
Synthetic peptide substrates for casein kinase 2. Assessment of minimum structural requirements for phosphorylation.
1988,
Pubmed Maridor,
Casein kinase II. cDNA sequences, developmental expression, and tissue distribution of mRNAs for alpha, alpha', and beta subunits of the chicken enzyme.
1991,
Pubmed Meek,
The p53 tumour suppressor protein is phosphorylated at serine 389 by casein kinase II.
1990,
Pubmed Meggio,
Structure and properties of casein kinase-2 from Saccharomyces cerevisiae. A comparison with the liver enzyme.
1986,
Pubmed Meggio,
Subunit structure and autophosphorylation mechanism of casein kinase-TS (type-2) from rat liver cytosol.
1984,
Pubmed Meggio,
Partially dephosphorylated phosphopeptide AcSer(P)-Ser(P)-Ser(P) is an excellent substrate for casein kinase-2.
1988,
Pubmed Meijer,
Differential regulation of histone H1 and ribosomal S6 kinases during sea star oocyte maturation.
1987,
Pubmed
,
Echinobase Meisner,
Molecular cloning of the human casein kinase II alpha subunit.
1989,
Pubmed Merril,
Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.
1981,
Pubmed Mulner-Lorillon,
M-phase-specific cdc2 protein kinase phosphorylates the beta subunit of casein kinase II and increases casein kinase II activity.
1990,
Pubmed
,
Echinobase Mulner-Lorillon,
Purification and characterization of a casein-kinase-II-type enzyme from Xenopus laevis ovary. Biological effects on the meiotic cell division of full-grown oocyte.
1988,
Pubmed Padmanabha,
Isolation, sequencing, and disruption of the yeast CKA2 gene: casein kinase II is essential for viability in Saccharomyces cerevisiae.
1990,
Pubmed Padmanabha,
Casein kinase II of yeast contains two distinct alpha polypeptides and an unusually large beta subunit.
1987,
Pubmed Pelech,
Identification of a major maturation-activated acetyl-CoA carboxylase kinase in sea star oocytes as p44mpk.
1991,
Pubmed
,
Echinobase Pelech,
Protein kinase cascades in meiotic and mitotic cell cycle control.
1990,
Pubmed
,
Echinobase Pinna,
Casein kinase 2: an 'eminence grise' in cellular regulation?
1990,
Pubmed Pyerin,
Catalytic and molecular properties of highly purified phosvitin/casein kinase type II from human epithelial cells in culture (HeLa) and relation to ecto protein kinase.
1987,
Pubmed Qi,
Heterogeneity of protein kinase NII. Multiple subunit-polypeptides.
1986,
Pubmed Sanghera,
Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.
1990,
Pubmed
,
Echinobase Saxena,
Isolation and sequencing of cDNA clones encoding alpha and beta subunits of Drosophila melanogaster casein kinase II.
1987,
Pubmed Sommercorn,
Activation of casein kinase II in response to insulin and to epidermal growth factor.
1987,
Pubmed Sternbach,
Purification of a yeast protein kinase sharing properties with type I and type II casein kinases.
1987,
Pubmed Takio,
Amino acid sequence of the beta subunit of bovine lung casein kinase II.
1987,
Pubmed Traugh,
Importance of subunit interactions in regulation of casein kinase II.
1990,
Pubmed Tuazon,
Casein kinase I and II--multipotential serine protein kinases: structure, function, and regulation.
1991,
Pubmed
