Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Muscle Res Cell Motil
1992 Dec 01;136:635-9. doi: 10.1007/bf01738253.
Show Gene links
Show Anatomy links
Sea urchin axonemal motion supported by fluorescent, ribose-modified analogues of ATP.
Omoto CK
.
???displayArticle.abstract???
The axonemal motion supported by fluorescent ribose-modified analogues, anthraniloyl ATP (Ant-ATP) and methylanthraniloyl ATP (Mant-ATP), was investigated. Ant-ATP and Mant-ATP supported good vigorous motion. A detailed study of the movement shows that the maximum beat frequencies (Vmax) were significantly lower with the analogues. However, Michaelis constants (Km) for beat frequency were also significantly lower than with ATP. Thus the net effect of changes in these two parameters, Vmax/Km, was similar for ATP and Ant-ATP and higher with Mant-ATP. Thus these fluorescent analogues are good substrates for axonemal movement. The consistently higher value of Vmax/Km, a measure of substrate selectivity, with Mant-ATP over Ant-ATP suggests a feature of the ribose binding site. Other significant differences in the movement with the fluorescent analogues are quantified in terms of kinetic measures of sliding velocity and bend propagation velocity.
Brokaw,
Computerized analysis of flagellar motility by digitization and fitting of film images with straight segments of equal length.
1990, Pubmed,
Echinobase
Brokaw,
Computerized analysis of flagellar motility by digitization and fitting of film images with straight segments of equal length.
1990,
Pubmed
,
Echinobase
Brokaw,
Automated methods for estimation of sperm flagellar bending parameters.
1984,
Pubmed
,
Echinobase
Brokaw,
Effects of viscosity and ATP concentration on the movement of reactivated sea-urchin sperm flagella.
1975,
Pubmed
,
Echinobase
Brokaw,
Sperm motility.
1986,
Pubmed
,
Echinobase
Cremo,
Interaction of myosin subfragment 1 with fluorescent ribose-modified nucleotides. A comparison of vanadate trapping and SH1-SH2 cross-linking.
1990,
Pubmed
Gibbons,
Studies on the adenosine triphosphatase activity of 14 S and 30 S dynein from cilia of Tetrahymena.
1966,
Pubmed
Gibbons,
Flagellar movement and adenosine triphosphatase activity in sea urchin sperm extracted with triton X-100.
1972,
Pubmed
,
Echinobase
Hiratsuka,
Distinct structures of ATP and GTP complexes in the myosin ATPase.
1984,
Pubmed
Hiratsuka,
New ribose-modified fluorescent analogs of adenine and guanine nucleotides available as substrates for various enzymes.
1983,
Pubmed
Inaba,
Anthraniloyl ATP, a fluorescent analog of ATP, as a substrate for dynein ATPase and flagellar motility.
1989,
Pubmed
,
Echinobase
Omoto,
2-Chloro adenosine triphosphate as substrate for sea urchin axonemal movement.
1989,
Pubmed
,
Echinobase
Penningroth,
Evidence for functional differences between two flagellar dynein ATPases.
1986,
Pubmed
,
Echinobase
Rosenfeld,
Reactions of 1-N6-ethenoadenosine nucleotides with myosin subfragment 1 and acto-subfragment 1 of skeletal and smooth muscle.
1984,
Pubmed
Shimizu,
Nucleotide specificity of the enzymatic and motile activities of dynein, kinesin, and heavy meromyosin.
1991,
Pubmed
,
Echinobase
Shimizu,
The substrate specificity of dynein from Tetrahymena cilia.
1987,
Pubmed
Takahashi,
Kinetic properties of dynein ATPase from Tetrahymena pyriformis. The initial phosphate burst of dynein ATPase and its interaction with ATP analogs.
1979,
Pubmed
