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The motile beta/IC1 subunit of sea urchin sperm outer arm dynein does not form a rigor bond.
Moss AG
,
Gatti JL
,
Witman GB
.
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We used in vitro translocation and cosedimentation assays to study the microtubule binding properties of sea urchin sperm outer arm dynein and its beta/IC1 subunit. Microtubules glided on glass-absorbed sea urchin dynein for a period of time directly proportional to the initial MgATP2- concentration and then detached when 70-95% of the MgATP2- was hydrolyzed. Detachment resulted from MgATP2- depletion, because (a) perfusion with fresh buffer containing MgATP2- reconstituted binding and gliding, (b) microtubules glided many minutes with an ATP-regenerating system at ATP concentrations which alone supported gliding for only 1-2 min, and (c) microtubules detached upon total hydrolysis of ATP by an ATP-removal system. The products of ATP hydrolysis antagonized binding and gliding; as little as a threefold excess of ADP/Pi over ATP resulted in complete loss of microtubule binding and translocation by the beta/IC1 subunit. In contrast to the situation with sea urchin dynein, microtubules ceased gliding but remained bound to glass-absorbed Tetrahymena outer arm dynein when MgATP2- was exhausted. Cosedimentation assays showed that Tetrahymena outer arm dynein sedimented with microtubules in an ATP-sensitive manner, as previously reported (Porter, M.E., and K. A. Johnson. J. Biol. Chem. 258: 6575-6581). However, the beta/IC1 subunit of sea urchin dynein did not cosediment with microtubules in the absence of ATP. Thus, this subunit, while capable of generating motility, lacks both structural and rigor-type microtubule binding.
Bell,
Structure of the dynein-1 outer arm in sea urchin sperm flagella. II. Analysis by proteolytic cleavage.
1982, Pubmed,
Echinobase
Bell,
Structure of the dynein-1 outer arm in sea urchin sperm flagella. II. Analysis by proteolytic cleavage.
1982,
Pubmed
,
Echinobase
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Brokaw,
Future directions for studies of mechanisms for generating flagellar bending waves.
1986,
Pubmed
,
Echinobase
Fox,
Direction of force generated by the inner row of dynein arms on flagellar microtubules.
1987,
Pubmed
,
Echinobase
Gatti,
Outer arm dynein from trout spermatozoa. Purification, polypeptide composition, and enzymatic properties.
1989,
Pubmed
Gibbons,
Organic anions stabilize the reactivated motility of sperm flagella and the latency of dynein 1 ATPase activity.
1985,
Pubmed
,
Echinobase
Gibbons,
A latent adenosine triphosphatase form of dynein 1 from sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Gibbons,
The molecular basis of flagellar motility in sea urchin spermatozoa.
1975,
Pubmed
,
Echinobase
Gibbons,
Properties of flagellar "rigor waves" formed by abrupt removal of adenosine triphosphate from actively swimming sea urchin sperm.
1974,
Pubmed
,
Echinobase
Gibbons,
Multiple nucleotide-binding sites in the sequence of dynein beta heavy chain.
1991,
Pubmed
,
Echinobase
Gibbons,
Relationship between the latent adenosine triphosphatase state of dynein 1 and its ability to recombine functionally with KCl-extracted sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Holzbaur,
ADP release is rate limiting in steady-state turnover by the dynein adenosinetriphosphatase.
1989,
Pubmed
Ishijima,
Flagellar movement of intact and demembranated, reactivated ram spermatozoa.
1987,
Pubmed
Johnson,
Pathway of the microtubule-dynein ATPase and the structure of dynein: a comparison with actomyosin.
1985,
Pubmed
Johnson,
Preparation and properties of dynein from Tetrahymena cilia.
1986,
Pubmed
Johnson,
The pathway of ATP hydrolysis by dynein. Kinetics of a presteady state phosphate burst.
1983,
Pubmed
King,
Structure of the alpha and beta heavy chains of the outer arm dynein from Chlamydomonas flagella. Masses of chains and sites of ultraviolet-induced vanadate-dependent cleavage.
1987,
Pubmed
King,
Outer-arm dynein from trout spermatozoa: substructural organization.
1990,
Pubmed
King,
Structure of the alpha and beta heavy chains of the outer arm dynein from Chlamydomonas flagella. Location of epitopes and protease-sensitive sites.
1988,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lee-Eiford,
Specific cleavage of dynein heavy chains by ultraviolet irradiation in the presence of ATP and vanadate.
1986,
Pubmed
,
Echinobase
Mitchell,
Binding of dynein 21 S ATPase to microtubules. Effects of ionic conditions and substrate analogs.
1981,
Pubmed
Mitchell,
Interactions of dynein arms with b subfibers of Tetrahymena cilia: quantitation of the effects of magnesium and adenosine triphosphate.
1980,
Pubmed
Moss,
The alpha subunit of sea urchin sperm outer arm dynein mediates structural and rigor binding to microtubules.
1992,
Pubmed
,
Echinobase
Ogawa,
Four ATP-binding sites in the midregion of the beta heavy chain of dynein.
1991,
Pubmed
,
Echinobase
Okuno,
Inhibition of movement of trition-demembranated sea-urchin sperm flagella by Mg2+, ATP4-, ADP and P1.
1979,
Pubmed
,
Echinobase
Okuno,
Inhibition and relaxation of sea urchin sperm flagella by vanadate.
1980,
Pubmed
,
Echinobase
Omoto,
Activation of the dynein adenosinetriphosphatase by microtubules.
1986,
Pubmed
Paschal,
Isolated flagellar outer arm dynein translocates brain microtubules in vitro.
1988,
Pubmed
,
Echinobase
Penningroth,
Effects of adenylyl imidodiphosphate, a nonhydrolyzable adenosine triphosphate analog, on reactivated and rigor wave sea urchin sperm.
1978,
Pubmed
,
Echinobase
Penningroth,
ATP formation from adenyl-5'-yl imidodiphosphate, a nonhydrolyzable ATP analog.
1980,
Pubmed
Pfister,
The photoaffinity probe 8-azidoadenosine 5'-triphosphate selectively labels the heavy chain of Chlamydomonas 12 S dynein.
1984,
Pubmed
Pfister,
Subfractionation of Chlamydomonas 18 S dynein into two unique subunits containing ATPase activity.
1984,
Pubmed
Porter,
Transient state kinetic analysis of the ATP-induced dissociation of the dynein-microtubule complex.
1983,
Pubmed
Porter,
Characterization of the ATP-sensitive binding of Tetrahymena 30 S dynein to bovine brain microtubules.
1983,
Pubmed
Porter,
Dynein structure and function.
1989,
Pubmed
Sale,
Direction of active sliding of microtubules in Tetrahymena cilia.
1977,
Pubmed
Sale,
Isolated beta-heavy chain subunit of dynein translocates microtubules in vitro.
1988,
Pubmed
,
Echinobase
Satir,
Studies on cilia. 3. Further studies on the cilium tip and a "sliding filament" model of ciliary motility.
1968,
Pubmed
Satir,
Mechanisms and controls of microtubule sliding in cilia.
1982,
Pubmed
Shimizu,
Kinetic evidence for multiple dynein ATPase sites.
1983,
Pubmed
Shimizu,
Nucleotide specificity of the enzymatic and motile activities of dynein, kinesin, and heavy meromyosin.
1991,
Pubmed
,
Echinobase
Shingyoji,
Local reactivation of Triton-extracted flagella by iontophoretic application of ATP.
1977,
Pubmed
,
Echinobase
Spungin,
Dynein arm attachment probed with a non-hydrolyzable ATP analog. Structural evidence for patterns of activity.
1987,
Pubmed
Summers,
Adenosine triphosphate-induced sliding of tubules in trypsin-treated flagella of sea-urchin sperm.
1971,
Pubmed
,
Echinobase
Takahashi,
Binding of 30s dynein with the B-tubule of the outer doublet of axonemes from Tetrahymena pyriformis and adenosine triphosphate-induced dissociation of the complex.
1978,
Pubmed
Tang,
Structure of the dynein-1 outer arm in sea urchin sperm flagella. I. Analysis by separation of subunits.
1982,
Pubmed
,
Echinobase
Toyoshima,
Chymotryptic digestion of Tetrahymena 22S dynein. I. Decomposition of three-headed 22S dynein to one- and two-headed particles.
1987,
Pubmed
Toyoshima,
Chymotryptic digestion of Tetrahymena ciliary dynein. II. Pathway of the degradation of 22S dynein heavy chains.
1987,
Pubmed
Vale,
One-dimensional diffusion of microtubules bound to flagellar dynein.
1989,
Pubmed
,
Echinobase
Vale,
Microtubule translocation properties of intact and proteolytically digested dyneins from Tetrahymena cilia.
1989,
Pubmed
Vale,
Rotation and translocation of microtubules in vitro induced by dyneins from Tetrahymena cilia.
1988,
Pubmed
Vallee,
Reversible assembly purification of microtubules without assembly-promoting agents and further purification of tubulin, microtubule-associated proteins, and MAP fragments.
1986,
Pubmed
Wais-Steider,
Effect of vanadate on gill cilia: switching mechanism in ciliary beat.
1979,
Pubmed
Warner,
Cation-induced attachment of ciliary dynein cross-bridges.
1978,
Pubmed
Warner,
Rebinding of Tetrahymena 13 S and 21 S dynein ATPases to extracted doublet microtubules. The inner row and outer row dynein arms.
1985,
Pubmed
Warner,
Binding stoichiometry of 21S dynein to A and B subfiber microtubules.
1982,
Pubmed
Witman,
Axonemal dyneins.
1992,
Pubmed
