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Mol Biol Rep
1975 Mar 01;21:27-34. doi: 10.1007/bf00357294.
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Buoyant density centrifugation of sea urchin embryo chromatin on sucrose-glucose gradient.
Ljiljana S
,
Koviljka K
.
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Unsheared chromatin isolated from sea urchin embryos was submitted to buoyant density centrifugation in sucrose-glucose gradients. The main peak of blastula chromatin was at a density position of 1.299 plus or minus 0.028 plus or minus 0.009 g ml -minus 1 whereas at gastrula stage a shift to a lower bouoyant density position of (1.276 plus or minus 0.021 plus or minus 0.007 g ml minus 1) was observed. Besides the main peak, a small band with a density of 1.18 g ml minus 1 was noticed. The lighter fraction differed from the heavy one in a higher histone to DNA ratio, a lower proportion of the F-1 histone, and a lower nonhistone to DNA ratio. The most pronounced developmental alterations of proteins were observed at the level of nonhistone protein patterns of the light fractions.
BURTON,
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
1956, Pubmed
BURTON,
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
1956,
Pubmed
Brutlag,
Properties of formaldehyde-treated nucleohistone.
1969,
Pubmed
Chan,
Isopycnic centrifugation of chromatin in renografin solutions.
1974,
Pubmed
Hossainy,
Isopycnic banding of chromatin in chloral hydrate gradients.
1973,
Pubmed
Ilyin,
Heterogeneity of deoxynucleoprotein particles as evidencec by ultracentrifugation of cesium chloride density gradient.
1969,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Marushige,
Properties of isolated chromatin from sea urchin embryo.
1967,
Pubmed
,
Echinobase
Nancock,
Separation by equilibrium centrifugation in CsC1 gradients of density--labelled and normal deoxyribonucleoprotein from chromatin.
1970,
Pubmed
Panyim,
High resolution acrylamide gel electrophoresis of histones.
1969,
Pubmed
Raynaud,
Buoyant density of native chromatin.
1972,
Pubmed
Smith,
A rapid method for the electrophoresis of chromatin proteins.
1973,
Pubmed
Weber,
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
1969,
Pubmed
Ševaljević,
Developmental changes of chromatin nonhistone proteins of sea urchin embryos.
1974,
Pubmed
,
Echinobase
Ševaljević,
Developmental changes of sea urchin histones.
1974,
Pubmed
,
Echinobase
