Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
???displayArticle.abstract???
Macromolecular components are released from sea urchin eggs when their metabolism is activated at fertilization or by incubation in ammonia. When the released material is dialyzed, concentrated, and added back to partially activated eggs the rate of protein synthesis is suppressed to the level of the unactivated egg. The surface proteins of the unfertilized eggs can be labeled with 125I by a lactoperoxidase procedure. When fertilized or activated with various parthenogenetic agents, 15-25% of the total labeled protein is released; most of the label is associated with a 150,000-dalton glycoprotein. The extent of metabolic activation, as assessed by measuring increased protein synthesis, is correlated with the amount of surface label released. Several other proteins are released during activation but are not labeled by the lactoperoxidase procedure in the intact cell. We have not yet identified which of these components is responsible for suppressing protein synthesis, nor do we know if any of the other metabolic changes of fertilization such as K+ conductance and DNA synthesis are also suppressed. We suggest that these released components are surface molecules involved in maintaining the low metabolic state occurring at the end of oogenesis and that removal of these components during fertilization results in the release of the suppression of the egg.
Cuatrecasas,
Membrane receptors.
1974,
Pubmed
Epel,
Methods for revoval of the vitelline membrane of sea urchin eggs. I. Use of dithiothreitol (Cleland Reagent).
1970,
Pubmed
,
Echinobase
Epel,
An analysis of the partial metabolic derepression of sea urchin eggs by ammonia: the existence of independent pathways.
1974,
Pubmed
,
Echinobase
Hartmann,
On the synthesis of glutamine before and after fertilization of the sea urchin, Strongylocentrotus purpuratus.
1974,
Pubmed
,
Echinobase
Huber,
Heterogeneity of the outer membrane of mitochondria.
1973,
Pubmed
Koch,
Reversible inhibition of protein synthesis in HeLa.
1974,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Longo,
A cytological study of the relation of the cortical reaction to subsequent events of fertilization in urethane-treated eggs of the sea urchin, Arbacia punctulata.
1970,
Pubmed
,
Echinobase
Mazia,
DNA synthesis turned on in unfertilized sea urchin eggs by treatment with NH4OH.
1974,
Pubmed
,
Echinobase
Murofushi,
Protein kinase in the sea urchin egg cortices. Its purification and characterization and some properties of an endogenous protein kinase in the cortices.
1974,
Pubmed
,
Echinobase
Rebhun,
Cleavage inhibition in marine eggs by puromycin and 6-dimethylaminopurine.
1973,
Pubmed
,
Echinobase
Shapiro,
Limited proteolysis of some egg surface components is an early event following fertilization of the sea urchin, Strongylocentrotus purpuratus.
1975,
Pubmed
,
Echinobase
Spiro,
Glycoproteins.
1973,
Pubmed
Steinhardt,
Development of K + -conductance and membrane potentials in unfertilized sea urchin eggs after exposure to NH 4 OH.
1973,
Pubmed
,
Echinobase
Steinhardt,
Activation of sea-urchin eggs by a calcium ionophore.
1974,
Pubmed
,
Echinobase
Studier,
Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
1973,
Pubmed
Vacquier,
The isolation of intact cortical granules from sea urchin eggs: calcium lons trigger granule discharge.
1975,
Pubmed
,
Echinobase
Yamada,
Isolation of a major cell surface glycoprotein from fibroblasts.
1974,
Pubmed
Yasumasu,
Periodic change in the content of adenosine 3'5'-cyclic monophosphate with close relation to the cycle of cleavage in the sea urchin egg.
1973,
Pubmed
,
Echinobase
