J Biochem 1976 Sep 01;803:637-40. doi: 10.1093/oxfordjournals.jbchem.a131320.

New types of gangliosides in starfish with sialic acid residues in the inner part of their carbohydrate chains.

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Three gangliosides were isolated from the starfish by Folch''s partition, dialysis, silicic acid column chromatography, Iatrobead column chromatography and preparative thin-layer chromatography. Partial acid hydrolysis of the three lipids in 0.1 N HCl at 80 degrees for 90 min liberated all the lactosyl ceramide and water-soluble degradation products, but no sialic acid. Gas-liquid chromatography of the trimethylsilylated methyl glycosides of the water-soluble products demonstrated the presence of arabinose in addition to the usual sugar units of gangliosides.

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