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Nucleic Acids Res
1978 Dec 01;512:4855-61. doi: 10.1093/nar/5.12.4855.
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Methylation of DNA in early development: 5-methyl cytosine content of DNA in sea urchin sperm and embryos.
Pollock JM
,
Swihart M
,
Taylor JH
.
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By separating formic acid hydrolysates with high pressure chromatography on an Aminex-10 column, we determined the ratio of 5-methyl cytosine to cytosine and other bases of DNA from sea urchin sperm and nuclei of embryos from early cleavage through pluteus stages. Contrary to several previous reports, we could not find any measurable changes in the methylation levels of embryonic nuclear DNAs at different stages of development. We also found no consistent differences between the methylation levels of sea urchin sperm and embryonic nuclei or the 5-methyl cytosine content of fish (Mugil cephalus) sperm and liver nuclei. While these measurements would not have detected subtle variations associated with differentiation, they would have indicated the gross changes previously reported for embryos or between sperm and somatic nuclei had those changes been present.
Adams,
Delayed methylation of DNA in developing sea urchin embryos.
1973, Pubmed,
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Adams,
Delayed methylation of DNA in developing sea urchin embryos.
1973,
Pubmed
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Bird,
Use of restriction enzymes to study eukaryotic DNA methylation: I. The methylation pattern in ribosomal DNA from Xenopus laevis.
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COMB,
METYLATION OF NUCLEIC ACIDS DURING SEA URCHIN EMBRYO DEVELOPMENT.
1965,
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Culp,
Methylated bases in DNA of animal origin.
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Cummings,
Methylated bases in DNA from Paramecium aurelia.
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Deutsch,
Analysis of 5-methylcytosine in DNA. I. Mass spectrometry.
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Gorovsky,
( 6 N)methyl adenine in the nuclear DNA of a eucaryote, Tetrahymena pyriformis.
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Grippo,
Methylation of DNA in developing sea urchin embryos.
1968,
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Hattman,
Comparative study of DNA methylation in three unicellular eucaryotes.
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Kappler,
The kinetics of DNA methylation in cultures of a mouse adrenal cell line.
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Kappler,
The 5-methylcytosine content of DNA: tissue specificity.
1971,
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Kemp,
A chemical and physical method for determining the complete base composition of plant DNA.
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Nass,
Differential methylation of mitochondrial and nuclear DNA in cultured mouse, hamster and virus-transformed hamster cells. In vivo and in vitro methylation.
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Rae,
Hydroxymethyluracil in eukaryote DNA: a natural feature of the pyrrophyta (dinoflagellates).
1976,
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Roeder,
Multiple ribonucleic acid polymerases and ribonucleic acid synthesis during sea urchin development.
1970,
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Scarano,
On methylation of DNA during development of the sea urchin embryo.
1965,
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Sneider,
Methylation of mammalian DNA: studies on Novikoff hepatoma cells in tissue culture.
1969,
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Vanyushin,
Rare bases in animal DNA.
1970,
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Vanyushin,
The content of 5-methylcytosine in animal DNA: the species and tissue specificity.
1973,
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