Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Biochem J
1978 Oct 01;1751:15-9. doi: 10.1042/bj1750015.
Show Gene links
Show Anatomy links
Isolation of heavy satellite deoxyribonucleic acid from sea-urchin spermatozoa.
Mishra NK
.
???displayArticle.abstract???
Two different methods were used to isolate high-molecular-weight heavy satellite DNA from the spermatozoa of the sea-urchin Lytechinus variegratus. Purification of satellite rDNA (ribosomal DNA) by selective denaturation of the spermatozoal DNA followed by a separation of the native and denatured DNA in an aqueous mixture of poly(ethylene glycol)/dextran is affected by the molecular weight of the spermatozoal DNA. Enrichment of satellite rDNA by selective precipitation of main-band DNA by poly-L-lysine is cumbersome but more suitable.
Alberts,
Efficient separation of single-stranded and double-stranded deoxyribonucleic acid in a dextran-polyethylene glycol two-phase system.
1967, Pubmed
Alberts,
Efficient separation of single-stranded and double-stranded deoxyribonucleic acid in a dextran-polyethylene glycol two-phase system.
1967,
Pubmed
Birnstiel,
The ribosomal RNA cistrons.
1971,
Pubmed
Brown,
Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.
1968,
Pubmed
Brown,
Methods of gene isolation.
1974,
Pubmed
Dawid,
Composition and structure of chromosomal and amplified ribosomal DNA's of Xenopus laevis.
1970,
Pubmed
Flamm,
Highly repetitive sequences of DNA in chromosomes.
1972,
Pubmed
GINOZA,
Mechanisms of inactivation of deoxyribonucleic acids by heat.
1961,
Pubmed
GINOZA,
On the inactivation of transforming DNA by temperatures below the melting point.
1961,
Pubmed
Graves,
Interaction of heat-denatured HeLa cell DNA with synthetic and natural polysaccharides.
1968,
Pubmed
Kirby,
Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria.
1967,
Pubmed
Patterson,
Sea urchin satellite deoxyribonucleic acid. Its large-scale isolation and hybridization with homologous ribosomal ribonucleic acid.
1970,
Pubmed
,
Echinobase
Polito,
DNA circularization: a molecular approach for the detection and isolation of tandemly duplicated genes.
1971,
Pubmed
STUDIER,
SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.
1965,
Pubmed
TAMM,
The formation apurinic acid from the desoxyribonucleic acid of calf thymus.
1952,
Pubmed
Walker,
"Repetitive" DNA in higher organisms.
1971,
Pubmed
