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ECB-ART-30542
Nucleic Acids Res 1978 Sep 01;59:3427-38. doi: 10.1093/nar/5.9.3427.
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Differential susceptibilities of DNA polymerases-alpha and -beta to polyanions.

Shimada T , Yamada M , Miwa M , Nagano H , Mano Y .


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The effects of various polyanions including synthetic polynucleotides on DNApolymerases-alpha and -beta from blastulae of the sea urchin Hemicentrotus pulcherrimus and HeLa cells were studied. Only DNA polymerase-alpha was inhibited by polyanions, such as polyvinyl sufate, dextran sulfate, heparin, poly(G), poly(I), poly(U) and poly(ADP-Rib). Of the various polynucleotides tested, poly(G) and poly(I) were the strongest inhibitors. Kinetic studies showed that the Ki value for poly(G) was 0.3 microgram/ml and that poly(G) had 20-fold higher affinity than activated DNA for the template-primer site of DNA polymerase-alpha. Poly(U) and poly(ADP-Rib) were also inhibitory, but they were one hundredth as inhibitory as poly(G) or poly(I). Poly(A), poly(C), poly(A).poly(U) AND POLY(I).poly(C) were not inhibitory to DNA polymerase-alpha. In contrast, DNA olymerase-beta was not affected at all by these polyanions under the same conditions.

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Genes referenced: LOC100887844 LOC115923832 polr3a

References [+] :
Bertazzoni, Levels of DNA polymerase-alpha and beta in normal and xeroderma pigmentosum fibroblasts. 1977, Pubmed