Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Nucleic Acids Res
1978 Sep 01;59:3427-38. doi: 10.1093/nar/5.9.3427.
Show Gene links
Show Anatomy links
Differential susceptibilities of DNA polymerases-alpha and -beta to polyanions.
Shimada T
,
Yamada M
,
Miwa M
,
Nagano H
,
Mano Y
.
???displayArticle.abstract???
The effects of various polyanions including synthetic polynucleotides on DNApolymerases-alpha and -beta from blastulae of the sea urchin Hemicentrotus pulcherrimus and HeLa cells were studied. Only DNA polymerase-alpha was inhibited by polyanions, such as polyvinyl sufate, dextran sulfate, heparin, poly(G), poly(I), poly(U) and poly(ADP-Rib). Of the various polynucleotides tested, poly(G) and poly(I) were the strongest inhibitors. Kinetic studies showed that the Ki value for poly(G) was 0.3 microgram/ml and that poly(G) had 20-fold higher affinity than activated DNA for the template-primer site of DNA polymerase-alpha. Poly(U) and poly(ADP-Rib) were also inhibitory, but they were one hundredth as inhibitory as poly(G) or poly(I). Poly(A), poly(C), poly(A).poly(U) AND POLY(I).poly(C) were not inhibitory to DNA polymerase-alpha. In contrast, DNA olymerase-beta was not affected at all by these polyanions under the same conditions.
Bertazzoni,
Levels of DNA polymerase-alpha and beta in normal and xeroderma pigmentosum fibroblasts.
1977, Pubmed
Bertazzoni,
Levels of DNA polymerase-alpha and beta in normal and xeroderma pigmentosum fibroblasts.
1977,
Pubmed
Bertazzoni,
Variations of DNA polymerase-alpha and -beta during prolonged stimulation of human lymphocytes.
1976,
Pubmed
Bollum,
Mammalian DNA polymerases.
1975,
Pubmed
Brown,
Effects of polyinosinic acid and polycytidylic acid on the deoxyribonucleic acid template activity of isolated nuclei and soluble chromatin from rat liver.
1972,
Pubmed
De Clercq,
Requirement of a stable secondary structure for the antiviral activity of polynucleotides.
1969,
Pubmed
Dicioccio,
Structure-activity relationships and kinetic analyses of polyribonucleotide inhibition of human cellular deoxynucleotide-polymerizing enzymes.
1977,
Pubmed
Dube,
Differential heat sensitivity of mammalian DNA polymerases.
1976,
Pubmed
Knopf,
HeLa cell DNA polymerase gamma: further purification and properties of the enzyme.
1976,
Pubmed
Loeb,
Purification and properties of deoxyribonucleic acid polymerase from nuclei of sea urchin embryos.
1969,
Pubmed
,
Echinobase
Mosbaugh,
Novikoff hepatoma deoxyribonucleic acid polymerase. Identification of a stimulatory protein bound to the beta-polymerase.
1977,
Pubmed
Noy,
HeLa cell DNA polymerases: the effect of cycloheximide in vivo and detection of a new form of DNA polymerase alpha.
1977,
Pubmed
Seki,
Differential effects of heparin on replicative DNA synthesis and unscheduled DNA synthesis.
1977,
Pubmed
Sugimura,
Studies on poly(adenosine diphosphate-ribose). XI. Purification of poly(adenosine diphosphate-ribose) on a hydroxylapatite column.
1971,
Pubmed
Suzuki-Hori,
DNA polymerase-beta from the nuclear fraction of sea urchin embryos: characterization of the purified enzyme.
1977,
Pubmed
,
Echinobase
Wang TS-F,
Identification of a new DNA polymerase activity in human KB cells.
1975,
Pubmed
Weissbach,
Eukaryotic DNA polymerases.
1977,
Pubmed