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J Cell Biol
1978 Oct 01;791:281-5. doi: 10.1083/jcb.79.1.281.
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Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels.
Adair WS
,
Jurivich D
,
Goodenough UW
.
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A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.
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