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An isolated surface complex consisting of the vitelline layer, plasma membrane, and attached secretory vesicles has been examined for its ability to bind sperm and to form the fertilization envelope. Isolated surface complexes (or intact eggs) fixed in glutaraldehyde and then washed in artificial sea water are capable of binding sperm in a species-specific manner. Sperm which bind to the isolated surface complex exhibit the acrosomal process only when they are associated with the exterior surface (vitelline layer) of the complex. Upon resuspension of the unfixed surface complex in artificial sea water, a limiting envelope is formed which, based on examination of thin sections and negatively stained surface preparations, is structurally similar to the fertilization envelope formed by the fertilized egg. These results suggest that the isolated egg surface complex retains the sperm receptor, as well as integrated functions for the secretion of components involved in assembly of the fertilization envelope.
Anderson,
Oocyte differentiation in the sea urchin, Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction.
1968, Pubmed,
Echinobase
Anderson,
Oocyte differentiation in the sea urchin, Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction.
1968,
Pubmed
,
Echinobase
Bryan,
On the reconstitution of the crystalline components of the sea urchin fertilization membrane.
1970,
Pubmed
,
Echinobase
Carroll,
Isolation and biological activity of the proteases released by sea urchin eggs following fertilization.
1975,
Pubmed
,
Echinobase
Chambers,
The activation of sea urchin eggs by the divalent ionophores A23187 and X-537A.
1974,
Pubmed
,
Echinobase
Decker,
A study of factors involved in induction of the acrosomal reaction in sperm of the sea urchin, Arbacia punctulata.
1976,
Pubmed
,
Echinobase
Detering,
Isolation and characterization of plasma membrane-associated cortical granules from sea urchin eggs.
1977,
Pubmed
,
Echinobase
ENDO,
Changes in the cortical layer of sea urchin eggs at fertilization as studied with the electron microscope. I. Clypeaster japonicus.
1961,
Pubmed
,
Echinobase
Epel,
The program of fertilization.
1977,
Pubmed
,
Echinobase
Foerder,
Release of ovoperoxidase from sea urchin eggs hardens the fertilization membrane with tyrosine crosslinks.
1977,
Pubmed
,
Echinobase
Glabe,
Isolation and characterization of the vitelline layer of sea urchin eggs.
1977,
Pubmed
,
Echinobase
Grey,
Evidence that the fertilization envelope blocks sperm entry in eggs of Xenopus laevis: interaction of sperm with isolated envelopes.
1976,
Pubmed
Kimura-Furukawa,
Induction of the acrosome reaction on the surface of de-jellied sea urchin eggs.
1978,
Pubmed
,
Echinobase
Longo,
Mechanism of soybean trypsin inhibitor induced polyspermy as determined by an analysis of refertilized sea urchin (Arbacia punctulata) eggs.
1974,
Pubmed
,
Echinobase
Runnström,
The vitelline membrane and cortical particles in sea urchin eggs and their function in maturation and fertilization.
1966,
Pubmed
,
Echinobase
Schmell,
Identification of a sperm receptor on the surface of the eggs of the sea urchin Arbacia punctulata.
1977,
Pubmed
,
Echinobase
Steinhardt,
Activation of sea-urchin eggs by a calcium ionophore.
1974,
Pubmed
,
Echinobase
Vacquier,
Methods for quantitating sea urchin sperm-egg binding.
1973,
Pubmed
,
Echinobase
Vacquier,
The isolation of intact cortical granules from sea urchin eggs: calcium lons trigger granule discharge.
1975,
Pubmed
,
Echinobase
Vacquier,
Protease released from sea urchin eggs at fertilization alters the vitelline layer and aids in preventing polyspermy.
1973,
Pubmed
,
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