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Proc Natl Acad Sci U S A
1979 Apr 01;764:1873-7. doi: 10.1073/pnas.76.4.1873.
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Inhibitors of membrane transport reduce lysosomal enzyme secretion from dogfish phagocytes and their killing of sea urchin eggs.
Dunham P
,
Arvan P
,
Falkow S
,
Hoffstein S
,
Weissmann G
.
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Blood phagocytes of the dogfish Mustelus canis attack oocytes of the sea urchin Arbacia punctulata, first provoking a surrogate fertilization response and then killing the eggs. To test the hypothesis that secretion of lysosomal contents is critical in this model of phagocyte-mediated cell injury, we studied effects of agents that modify lysosomal enzyme secretion. Inhibitors of membrane transport (>0.1 mM) inhibited postphagocytic secretion of lysosomal beta-glucuronidase from dogfish phagocytes: phloretin > ethacrynate > furosemide > 4-acetamido-4''-isothiocyanostilbene-2,2''-disulfonic acid > pyridoxal phosphate > ouabain. The same order of activity was found for inhibition by these agents of killing of Arbacia eggs by phagocytes. Cell activation (fertilization response) and cytotoxicity were quantitated both morphologically and by measurements of enzyme (beta-glucuronidase, catalase) release. The agents neither inhibited fertilization responses of eggs to calcium ionophore A23187 nor impaired their viability. Vital staining demonstrated that ethacrynate prevented phagocytes from degranulating upon contact with zymosan particles. The data not only suggest that agents primarily known for their capacity to inhibit membrane transport systems can inhibit lysosomal enzyme secretion from phagocytes but also support the hypothesis that secretion of lysosomal contents mediates activation and killing of target cells in phagocyte-mediated tissue injury.
Andersen,
Effect of phloretin on the permeability of thin lipid membranes.
1976, Pubmed
Andersen,
Effect of phloretin on the permeability of thin lipid membranes.
1976,
Pubmed
BEERS,
A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase.
1952,
Pubmed
Brazy,
Furosemide inhibition of chloride transport in human red blood cells.
1976,
Pubmed
Brown,
Role of anions in parathyroid hormone release from dispersed bovine parathyroid cells.
1978,
Pubmed
Cabantchik,
Pyridoxal phosphate. An anionic probe for protein amino groups exposed on the outer and inner surfaces of intact human red blood cells.
1975,
Pubmed
Christoffersen,
Calcium ion activity in physiological salt solutions: influence of anions substituted for chloride.
1975,
Pubmed
Gale,
Polymorphonuclear leukocytes in antibody-dependent cellular cytotoxicity.
1975,
Pubmed
Goldstein,
Influence of divalent cations upon complement-mediated enzyme release from human polymorphonuclear leukocytes.
1975,
Pubmed
Goldstein,
Independent effects of IgG and complement upon human polymorphonuclear leukocyte function.
1976,
Pubmed
Henson,
Interaction of cells with immune complexes: adherence, release of constituents, and tissue injury.
1971,
Pubmed
Hoffstein,
Mechanisms of lysosomal enzyme release from leukocytes. IV. Interaction of monosodium urate crystals with dogfish and human leukocytes.
1975,
Pubmed
Knauf,
Chemical modification of membranes. II. Permeation paths for sulfhydryl agents.
1971,
Pubmed
Korchak,
Changes in membrane potential of human granulocytes antecede the metabolic responses to surface stimulation.
1978,
Pubmed
Magro,
Ethacrynic acid inhibitable Ca2+ and Mg2+-activated membrane adenosine triphosphatase in rat mast cells.
1977,
Pubmed
Motais,
Inhibitory effect of ethacrynic acid on chloride permeability.
1976,
Pubmed
Pazoles,
Evidence for stimulation of anion transport in ATP-evoked transmitter release from isolated secretory vesicles.
1978,
Pubmed
Pollard,
Evidence for control of serotonin secretion from human platelets by hydroxyl ion transport and osmotic lysis.
1977,
Pubmed
Schwartz,
The sodium-potassium adenosine triphosphatase: pharmacological, physiological and biochemical aspects.
1975,
Pubmed
Showell,
The effects of extracellular K+, Na+ and Ca++ on lysosomal enzyme secretion from polymorphonuclear leukocytes.
1977,
Pubmed
Simchowitz,
Lectin-dependent neutrophil-mediated cytotoxicity. II. Possible mechanisms.
1976,
Pubmed
Ulmer,
Metalloenzymes and myocardial infarction. II. Malic and lactic dehydrogenase activities and zinc concentrations in serum.
1956,
Pubmed
Vittoria,
[Action of furosemide on vitro in platelet aggregation by ADP, adrenaline or collagen].
1974,
Pubmed
Weissmann,
Attack of sea urchin eggs by dogfish phagocytes: model of phagocyte-mediated cellular cytotoxicity.
1978,
Pubmed
,
Echinobase
Weissmann,
A general method for the introduction of enzymes, by means of immunoglobulin-coated liposomes, into lysosomes of deficient cells.
1975,
Pubmed
Weissmann,
Mechanisms of lysosomal enzyme release from leukocytes exposed to immune complexes and other particles.
1971,
Pubmed
