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???displayArticle.abstract??? Bindin is an insoluble protein coating the sperm acrosome process and mediating the adhesion of sperm to sea urchin eggs. Milligrams of bindin have been isolated. Here we report the identification, isolation, and partial characterization of a high molecular weight, trypsin-sensitive glycoprotein fraction from the sea urchin egg surface having species-specific affinity for bindin. This glycoprotein may be the egg surface receptor for bindin. The bindin receptor was released from 125-I-labeled eggs by parthenogenetic activation of eggs with ionophore A23187 in the presence of soybean trypsin inhibitor. The receptor has an isoelectric point of 4.02 and a molecular weight in sea water greater than or equal to 5 X 10(6), suggesting that it is an aggregate. It contains 34% neutral sugars, which are galactose and mannose.
Aketa,
Physiological studies on the sperm surface component responsible for sperm-egg bonding in sea urchin fertilization. I. Effect of sperm-binding protein on the fertilizing capacity of sperm.
1973, Pubmed,
Echinobase
Aketa,
Physiological studies on the sperm surface component responsible for sperm-egg bonding in sea urchin fertilization. I. Effect of sperm-binding protein on the fertilizing capacity of sperm.
1973,
Pubmed
,
Echinobase
Carroll,
Isolation and biological activity of the proteases released by sea urchin eggs following fertilization.
1975,
Pubmed
,
Echinobase
Collins,
The role of calcium ions in the acrosome reaction of sea urchin sperm: regulation of exocytosis.
1977,
Pubmed
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Echinobase
Decker,
A study of factors involved in induction of the acrosomal reaction in sperm of the sea urchin, Arbacia punctulata.
1976,
Pubmed
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Echinobase
Fricke,
Tritosol: a new scintillation cocktail based on Triton X-100.
1975,
Pubmed
Glabe,
Isolation and characterization of the vitelline layer of sea urchin eggs.
1977,
Pubmed
,
Echinobase
Glabe,
Species specific agglutination of eggs by bindin isolated from sea urchin sperm.
1977,
Pubmed
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Echinobase
Gwatkin,
Heat sensitivity of the cortical granule protease from hamster eggs.
1974,
Pubmed
Kim,
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1967,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Schmell,
Identification of a sperm receptor on the surface of the eggs of the sea urchin Arbacia punctulata.
1977,
Pubmed
,
Echinobase
Steinhardt,
Activation of sea-urchin eggs by a calcium ionophore.
1974,
Pubmed
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Echinobase
Summers,
Species-specificity of acrosome reaction and primary gamete binding in echinoids.
1975,
Pubmed
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Echinobase
Terho,
Method for determination of the sulfate content of glycosaminoglycans.
1971,
Pubmed
Vacquier,
Isolation of bindin: the protein responsible for adhesion of sperm to sea urchin eggs.
1977,
Pubmed
,
Echinobase
Vacquier,
Sea urchin eggs release protease activity at fertilization.
1972,
Pubmed
,
Echinobase
Vacquier,
Protease released from sea urchin eggs at fertilization alters the vitelline layer and aids in preventing polyspermy.
1973,
Pubmed
,
Echinobase
Vacquier,
Methods for quantitating sea urchin sperm-egg binding.
1973,
Pubmed
,
Echinobase
