Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Cell Biol
1979 Jan 01;801:211-8. doi: 10.1083/jcb.80.1.211.
Show Gene links
Show Anatomy links
Calcium-binding modulator protein from the unfertilized egg of the sea urchin Arbacia punctulata.
Head JF
,
Mader S
,
Kaminer B
.
???displayArticle.abstract???
We have purified and partly characterized a calcium-binding protein from the unfertilized egg of the sea urchin Arbacia punctulata. This protein closely resembles the calcium-binding modulator protein of bovine brain in its molecular weight, electrophoretic mobility, amino acid analysis, and peptide map. It activates bovine brain phosphodiesterase in the presence of calcium but has no effect on the phosphodiesterase of the Arbacia egg. Densitometric scanning of acrylamide gels of arbacia egg homogenates shows the modulator protein to represent 0.1% of the total protein of the egg. At 10(-4) M free calcium, the protein binds four calcium ions per 17,000-dalton molecule. We have used a column of rabbit skeletal muscle troponin-I covalently coupled to Sepharose 4B as an affinity column to selectively purify the Arbacia egg calcium-binding protein. This column has also been used to purify bovine brain modulator protein and may prove of general use in isolating similar proteins from other sources. The technique may be particularly helpful when only small quantities of starting material are available.
Amphlett,
Effect of the troponin C-like protein from bovine brain (brain modulator protein) on the Mg2+-stimulated ATPase of skeletal muscle actinomyosin.
1976, Pubmed
Amphlett,
Effect of the troponin C-like protein from bovine brain (brain modulator protein) on the Mg2+-stimulated ATPase of skeletal muscle actinomyosin.
1976,
Pubmed
Cheung,
Cyclic 3',5'-nucleotide phosphodiesterase. Demonstration of an activator.
1970,
Pubmed
Fine,
Troponin C in brain.
1975,
Pubmed
Head,
The interaction of the calcium-binding protein (troponin C) with bivalent cations and the inhibitory protein (troponin I).
1974,
Pubmed
Head,
Affinity-chromatographic isolation and some properties of troponin C from different muscle types.
1977,
Pubmed
Jones,
High levels of a calcium-dependent modulator protein in spermatozoa and its similarity to brain modulator protein.
1978,
Pubmed
,
Echinobase
Kane,
Preparation and purification of polymerized actin from sea urchin egg extracts.
1975,
Pubmed
,
Echinobase
Klee,
Purification of cyclic 3',5'-nucleotide phosphodiesterase inhibitory protein by affinity chromatography on activator protein coupled to Sepharose.
1978,
Pubmed
Kuo,
Bovine adrenal medulla troponin-C. Demonstration of a calcium-dependent conformational change.
1976,
Pubmed
Mabuchi,
A myosin-like protein in the cortical layer of the sea urchin egg.
1973,
Pubmed
,
Echinobase
Nath,
Studies on cyclic AMP phosphodiesterase in sea urchin eggs.
1974,
Pubmed
,
Echinobase
Ridgway,
Free calcium increases explosively in activating medaka eggs.
1977,
Pubmed
Smoake,
Cyclic 3',5'-nucleotide phosphodiesterase. Distribution and developmental changes of the enzyme and its protein activator in mammalian tissues and cells.
1974,
Pubmed
Steinhardt,
Is calcium ionophore a universal activator for unfertilised eggs?
1974,
Pubmed
,
Echinobase
Steinhardt,
Intracellular calcium release at fertilization in the sea urchin egg.
1977,
Pubmed
,
Echinobase
Stephens,
Fluorescent thin-layer peptide mapping for protein identification and comparison in the subnanomole range.
1978,
Pubmed
Teo,
Mechanism of activation of a cyclic adenosine 3':5'-monophosphate phosphodiesterase from bovine heart by calcium ions. Identification of the protein activator as a Ca2+ binding protein.
1973,
Pubmed
Waisman,
The distribution of the Ca++-dependent protein activator of cyclic nucleotide phosphodiesterase in invertebrates.
1975,
Pubmed
Waisman,
Purification and characterization of a Ca2+-binding protein in Lumbricus terrestris.
1978,
Pubmed
Wang,
Modulator binding protein. Bovine brain protein exhibiting the Ca2+-dependent association with the protein modulator of cyclic nucleotide phosphodiesterase.
1977,
Pubmed
Watterson,
Structural similarities between the Ca2+-dependent regulatory proteins of 3':5'-cyclic nucleotide phosphodiesterase and actomyosin ATPase.
1976,
Pubmed
Weber,
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
1969,
Pubmed
Yagi,
Identification of an activator protein for myosin light chain kinase as the Ca2+-dependent modulator protein.
1978,
Pubmed
