Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
The effect of myosin antibody on the division of starfish blastomeres.
Mabuchi I, Okuno M.
???displayArticle.abstract???
Antiserum against starfish egg myosin was produced in rabbits. Antibody specificity to myosin was demonstrated by Ouchterlony''s immunodiffusion test and by immunoelectrophoresis in the presence of sodium dodecylsulfate (SDS). The latter technique showed that the antibody binds to both heavy and light chains of egg myosin. Furthermore, the antibody reacted with starfish sperm mysosin and starfish adult muscle myosin at both the heavy and light chains. It did not react with bovine platelet mysosin or rabbit skeletal muscle myosin in Ouchterlony''s test; however, a weak reaction was observed in the presence of SDS between the antibody and these myosin heavy chains. Ca- and Mg-ATPase activities of egg myosin were not affected by the antibody, but it did inhibit actin-activated ATPase activity of egg myosin. Microinjection of the antibody into blastomeres of starfish eggs at the two-cell stage was carried out. Anti-egg myosin gamma-globulin inhibited the subsequent cleavages at an amount of more than 0.3 ng when injected at interphase. The inhibition was reduced when the injection was carried out near the initiation of cleavage. At the onset of the second cleavage the antibody was not inhibitory; however, an appropriate amount inhibited the third cleavage. Although the disappearance of the nuclear membrane was observed in the presence of the antibody, the formation of the mitotic apparatus was more or less disturbed. However the formation of daughter nuclei seemed to be scarcely affected by the antibody except that the distance between the nuclei was significantly smaller than normal.
Arnold,
Cleavage furrow formation in a telolecithal egg (Loligo pealii). I. Filaments in early furrow formation.
1969, Pubmed
Arnold,
Cleavage furrow formation in a telolecithal egg (Loligo pealii). I. Filaments in early furrow formation.
1969,
Pubmed Arnold,
Cleavage furrow formation in a telolecithal egg (Loligo pealii). II. Direct evidence for a contraction of the cleavage furrow base.
1971,
Pubmed Bluemink,
The first cleavage of the amphibian egg. An electron microscope study of the onset of cytokinesis in the egg of Ambystoma mexicanum.
1970,
Pubmed Forer,
An actin-like component in spermatocytes of a crane fly (Nephrotoma suturalis Loew). I. The spindle.
1972,
Pubmed Forer,
An actin-like component in spermatocytes of a crane fly (Nephrotoma suturalis Loew). II. The cell cortex.
1972,
Pubmed Fujiwara,
Fluorescent antibody localization of myosin in the cytoplasm, cleavage furrow, and mitotic spindle of human cells.
1976,
Pubmed Gawadi,
Actin in the mitotic spindle.
1971,
Pubmed Hinkley,
Heavy meromyosin-binding filaments in the mitotic apparatus of mammaliam cells.
1974,
Pubmed Hiramoto,
Analysis of cleavage stimulus by means of micromanipulation of sea urchin eggs.
1971,
Pubmed
,
Echinobase Hiramoto,
A method of microinjection.
1974,
Pubmed HIRAMOTO,
FURTHER STUDIES ON CELL DIVISION WITHOUT MITOTIC APPARATUS IN SEA URCHIN EGGS.
1965,
Pubmed
,
Echinobase Ishikawa,
Formation of arrowhead complexes with heavy meromyosin in a variety of cell types.
1969,
Pubmed Lowey,
Light chains from fast and slow muscle myosins.
1971,
Pubmed LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed Mabuchi,
A myosin-like protein in the cortical layer of cleaving starfish eggs.
1974,
Pubmed
,
Echinobase Mabuchi,
A myosin-like protein in the cortical layer of the sea urchin egg.
1973,
Pubmed
,
Echinobase Mabuchi,
Isolation of myosin from starfish sperm heads.
1976,
Pubmed
,
Echinobase Masaki,
Immunochemical comparison of myosins from chicken cardiac, fast white, slow red, and smooth muscle.
1974,
Pubmed MOMMAERTS,
Reversible polymerization and ultracentrifugal purification of actin.
1951,
Pubmed Obinata,
The subunit structure of myosin from skeletal muscle of the early chick embryo.
1976,
Pubmed Perry,
Actin-like filaments in the cleavage furrow of newt egg.
1971,
Pubmed Pollard,
Human platelet myosin. I. Purification by a rapid method applicable to other nonmuscle cells.
1974,
Pubmed Rappaport,
Reversal of chemical cleavage inhibition in echinoderm eggs.
1971,
Pubmed
,
Echinobase Rappaport,
Cell division: direct measurement of maximum tension exerted by furrow of echinoderm eggs.
1967,
Pubmed
,
Echinobase Rappaport,
Cytokinesis in animal cells.
1971,
Pubmed
,
Echinobase Sanger,
Changing patterns of actin localization during cell division.
1975,
Pubmed Sanger,
Presence of actin during chromosomal movement.
1975,
Pubmed Sarkar,
Light chains of myosins from white, red, and cardiac muscles.
1971,
Pubmed Schroeder,
The contractile ring. I. Fine structure of dividing mammalian (HeLa) cells and the effects of cytochalasin B.
1970,
Pubmed Schroeder,
The contractile ring. II. Determining its brief existence, volumetric changes, and vital role in cleaving Arbacia eggs.
1972,
Pubmed
,
Echinobase Schroeder,
Actin in dividing cells: contractile ring filaments bind heavy meromyosin.
1973,
Pubmed Schroeder,
Cytokinesis: filaments in the cleavage furrow.
1968,
Pubmed Scott,
Filaments in the division furrow of mouse mammary cells.
1970,
Pubmed Selman,
Ultrastructural changes in the surface layers of the newt's egg in relation to the mechanism of its cleavage.
1970,
Pubmed Szollosi,
Cortical cytoplasmic filaments of cleaving eggs: a structural element corresponding to the contractile ring.
1970,
Pubmed Tucker,
Microtubules and a contractile ring of microfilaments associated with a cleavage furrow.
1971,
Pubmed
