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Echinobase
ECB-ART-30057
Biochim Biophys Acta 1976 Jan 23;4221:225-30. doi: 10.1016/0005-2744(76)90021-8.
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Inhibition of axoneme and dynein ATPase from sea urchin sperm by free ATP.

Hayashi M .


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Sea urchin sperm flagellar ATPase (EC 3.6.1.3) has magnesium-ATP as an effective substrate and is inhibited by free ATP. The inhibition is prevented by high concentration of KCl or NaCl. 0.4 M KCl extracts 48% of ATPase activity from axoneme. The 0.4 M KCl extract and 0.4 M KCl-treated axoneme are also inhibited by free ATP and this inhibition is reversed by KCl. Dynein purified twice by sucrose density gradient centrifugation is also inhibited by free ATP; this inhibition is also reversed by KCl.

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Genes referenced: dnah3 LOC100887844 LOC581395