ECB-ART-30010
Biochim Biophys Acta
1979 Jan 26;5611:17-28. doi: 10.1016/0005-2787(79)90486-6.
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Identification of gamma-like DNA polymerase from sea urchin embryos.
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A gamma-like DNA polymerase devoid of DNA polymerase-alpha and -beta activities was prepared from the nuclear fraction of blastulae of the sea urchin, Hemicentrotus pulcherrimus. The enzyme sedimented at the position of an approximate sedimentation coefficient of 3.3 S under high salt conditions by sucrose gradient centrifugation. An isoelectric point was determined to be pH 5.8. The enzyme activity was sensitive to sulfhydryl blocking reagents. Poly(rA) . oligo(dT)12--18 followed by poly(dA) . oligo(dT)12--18 was effectively utilized as a template-primer. From the above results, this polymerase seems to resemble the vertebrate DNA polymerase-gamma.
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Genes referenced: LOC100887844 LOC115923832 polr3a