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Proc Natl Acad Sci U S A
1975 Mar 01;723:1122-6. doi: 10.1073/pnas.72.3.1122.
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Assembly of chick brain tubulin onto flagellar microtubules from Chlamydomonas and sea urchin sperm.
Binder LI
,
Dentler WL
,
Rosenbaum JL
.
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Flagellar microtubules from Chlamydomonas and sea urchin sperm were used as in vitro assembly sites for chick brain tubulin. Brain microtubules assembled onto the A-tubules, central tubules, and, to a limited extent, onto the distal ends of the axonemes at low tubulin concentrations and onto distal and proximal ends at high tubulin concentrations; however, the rate of assembly onto the distal end was always greater. The rate of neurotubule assembly onto axonemes was shown to be dependent upon tubulin concentration and a forward rate constant for assembly was determined.
