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Proc Natl Acad Sci U S A
1973 Dec 01;7012:3594-8. doi: 10.1073/pnas.70.12.3594.
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Isolation and partial characterization of alpha and beta-tubulin from outer doublets of sea-urchin sperm and microtubules of chick-embryo brain.
Luduena RF
,
Woodward DO
.
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Two kinds of tubulin (alpha and beta) have been described in microtubules from many different systems. In this study a discontinuous acrylamide-gel system containing sodium dodecyl sulfate was used to separate milligram quantities of alpha- and beta-tubulin from microtubules of chick-embryo brain and from outer doublets of sea-urchin sperm. The isolated tubulins were characterized by peptide mapping and automated sequencing of the first 25 NH(2)-terminal amino acids. Our results show that alpha- and beta-tubulin are related but distinctly different proteins and that each one has been highly conserved in the course of evolution.
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