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Echinobase
ECB-ART-42520
Mol Biol Cell 2012 Sep 01;2318:3663-72. doi: 10.1091/mbc.E12-06-0438.
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Actin polymerization controls the activation of multidrug efflux at fertilization by translocation and fine-scale positioning of ABCB1 on microvilli.

Whalen K , Reitzel AM , Hamdoun A .


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Fertilization changes the structure and function of the cell surface. In sea urchins, these changes include polymerization of cortical actin and a coincident, switch-like increase in the activity of the multidrug efflux transporter ABCB1a. However, it is not clear how cortical reorganization leads to changes in membrane transport physiology. In this study, we used three-dimensional superresolution fluorescence microscopy to resolve the fine-scale movements of the transporter along polymerizing actin filaments, and we show that efflux activity is established after ABCB1a translocates to the tips of the microvilli. Inhibition of actin polymerization or bundle formation prevents tip localization, resulting in the patching of ABCB1a at the cell surface and decreased efflux activity. In contrast, enhanced actin polymerization promotes tip localization. Finally, interference with Rab11, a regulator of apical recycling, inhibits activation of efflux activity in embryos. Together our results show that actin-mediated, short-range traffic and positioning of transporters at the cell surface regulates multidrug efflux activity and highlight the multifaceted roles of microvilli in the spatial distribution of membrane proteins.

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Species referenced: Echinodermata
Genes referenced: abcb1 LOC100887844 LOC115919910 LOC577676 LOC586591 LOC590297 LOC590502 rab11
???displayArticle.antibodies??? abcb1 Ab1 abcb1 Ab2 rab11 Ab1


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References [+] :
Abascal, ProtTest: selection of best-fit models of protein evolution. 2005, Pubmed