Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Proc Natl Acad Sci U S A
1989 Apr 01;867:2128-32. doi: 10.1073/pnas.86.7.2128.
Show Gene links
Show Anatomy links
Cloning of the mRNA for the protein that crosslinks to the egg peptide speract.
Dangott LJ
,
Jordan JE
,
Bellet RA
,
Garbers DL
.
???displayArticle.abstract???
An apparent receptor for the egg peptide speract (Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly) was identified by covalently coupling a radiolabeled speract analogue to intact spermatozoa and was then purified by DEAE-Sepharose chromatography and preparative gel electrophoresis after solubilization with Lubrol PX. The purified, crosslinked protein was digested with Staphylococcus aureus V8 protease and a resultant peptide, purified from polyacrylamide slab gel slices, was shown to have the amino acid sequence Val-Ser-Ala-Pro-Phe-Asp-Leu-Glu-Ala-Pro-Phe-Ile-Ile-Asp-Gly-Ile. Polyclonal antiserum, generated against a synthetic peptide that corresponded to the above sequence, immunoprecipitated the radiolabeled crosslinked protein and reacted with a Mr 77,000 protein on immunoblots, demonstrating that the sequenced peptide originated from the apparent receptor. A clone containing a 2.5-kilobase insert was subsequently isolated from a sea urchin testis cDNA library that contained DNA sequences encoding an open reading frame of 532 amino acids that included the above peptide sequence. The deduced amino acid sequence suggests that the protein contains a 26-residue amino-terminal signal peptide, a large extracellular domain relatively rich in cysteine (5%) that includes a four-fold repeat of about 115 amino acids, a single membrane-spanning region, and only 12 amino acid residues extending into the cytoplasm. Analysis of total RNA from Strongylocentrotus purpuratus testis by Northern blot revealed a 2.5-kilobase RNA. Preliminary data show the presence of hybridizing RNA of the same apparent size in other sea urchin species, including Arbacia punctulata, which does not respond to speract.
Bentley,
Retention of the speract receptor by isolated plasma membranes of sea urchin spermatozoa.
1986, Pubmed,
Echinobase
Bentley,
Retention of the speract receptor by isolated plasma membranes of sea urchin spermatozoa.
1986,
Pubmed
,
Echinobase
Bentley,
Receptor-mediated activation of spermatozoan guanylate cyclase.
1986,
Pubmed
,
Echinobase
Cathala,
A method for isolation of intact, translationally active ribonucleic acid.
1983,
Pubmed
Cleveland,
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
1977,
Pubmed
Dangott,
Identification and partial characterization of the receptor for speract.
1984,
Pubmed
,
Echinobase
Garbers,
The amino acid sequence and chemical synthesis of speract and of speract analogues.
1982,
Pubmed
,
Echinobase
Hansbrough,
Speract. Purification and characterization of a peptide associated with eggs that activates spermatozoa.
1981,
Pubmed
,
Echinobase
Hunkapiller,
Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.
1983,
Pubmed
Kishimoto,
Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family.
1987,
Pubmed
Kozak,
Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.
1981,
Pubmed
Krikos,
Sensory transducers of E. coli are composed of discrete structural and functional domains.
1983,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Ohtsuka,
An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions.
1985,
Pubmed
Repaske,
A hydrogen ion flux mediates stimulation of respiratory activity by speract in sea urchin spermatozoa.
1983,
Pubmed
,
Echinobase
Sabatini,
Mechanisms for the incorporation of proteins in membranes and organelles.
1982,
Pubmed
Sanger,
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
1980,
Pubmed
Schackmann,
Alteration of intracellular [Ca2+] in sea urchin sperm by the egg peptide speract. Evidence that increased intracellular Ca2+ is coupled to Na+ entry and increased intracellular pH.
1986,
Pubmed
,
Echinobase
Schneider,
Use of antipeptide antibodies to demonstrate external orientation of the NH2-terminus of the low density lipoprotein receptor in the plasma membrane of fibroblasts.
1983,
Pubmed
Shimomura,
Covalent coupling of a resact analogue to guanylate cyclase.
1986,
Pubmed
,
Echinobase
Shimomura,
Derivatives of speract are associated with the eggs of Lytechinus pictus sea urchins.
1986,
Pubmed
,
Echinobase
Singh,
Membrane guanylate cyclase is a cell-surface receptor with homology to protein kinases.
1988,
Pubmed
,
Echinobase
Suzuki,
Purification and the primary structure of sperm-activity peptides from the jelly coat of sea urchin eggs.
1981,
Pubmed
,
Echinobase
Suzuki,
A peptide associated with eggs causes a mobility shift in a major plasma membrane protein of spermatozoa.
1984,
Pubmed
,
Echinobase
Ullrich,
Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells.
1984,
Pubmed
Ullrich,
Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes.
1985,
Pubmed
Ward,
Chemotaxis of Arbacia punctulata spermatozoa to resact, a peptide from the egg jelly layer.
1985,
Pubmed
,
Echinobase
Watson,
Compilation of published signal sequences.
1984,
Pubmed
Wray,
Silver staining of proteins in polyacrylamide gels.
1981,
Pubmed
Yamamoto,
The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA.
1984,
Pubmed