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BMC Complement Altern Med
2015 Feb 05;15:14. doi: 10.1186/s12906-015-0533-7.
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Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats.
Fahmy SR
.
Abstract
BACKGROUND: Holothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast. The present study aims to assess the anti-oxidative and anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct ligation in male albino rats.
METHODS: Fifty four male Wistar albino rats were assigned into two main groups, the Sham-operated control and bile duct ligated (BDL) group. After 14 days of surgery, the animals of the group I (Sham control) received distilled water only for 7, 14 and 28 days. Second group (BDL group) was divided into 2 subgroups, animals of these subgroups treated for 7, 14 and 28 consecutive days as follow: subgroup I (BDL), rats of this subgroup administered distilled water orally. Subgroup II (HaE), animals of this subgroup treated orally with HaE (200 mg/kg body weight).
RESULTS: The HaE revealed significant antifibrotic effect as evident by decreasing the levels of total conjugated and unconjugated bilirubin and the activities of serum aminotransferases (ASAT and ALAT) and alkaline phosphatase (ALP) as well as malondialdehyde (MDA) level, and increasing the serum albumin, glutathione reduced (GSH) levels. Treatment with HaE normalized the antioxidant enzyme, glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activities activities.
CONCLUSION: The present prospective study correlated the antifibrotic effect of HaE to its direct antioxidant effect that can be related to its contents of phenolic compounds specially chlorogenic acid, pyrogallol, rutin and coumaric acid.
Figure 2.
High-performance liquid chromatographic analysis of
Holothuria arenicola
extract.
Figure 4.
Histological study of picrosirius red -stained liver sections (200×) of Sham, BDL and
Holothuria arenicola
extract (HaE) treated rats. Rats were grouped: group 1, rats treated for 7Â days; group 2, rats treated for 14Â days and group 3, rats treated for 28Â days. Liver sections from: (A) Control (Sham operated) rats; (B) bile duct-ligated rats; (C) bile duct-ligated rats and treated with Holothuria arenicola extract (HaE). Arrows indicated collagen deposition stained by picrosirius red.
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