Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mol Biol Cell
1994 Jan 01;51:57-70. doi: 10.1091/mbc.5.1.57.
Show Gene links
Show Anatomy links
Phylogeny and expression of axonemal and cytoplasmic dynein genes in sea urchins.
Gibbons BH
,
Asai DJ
,
Tang WJ
,
Hays TS
,
Gibbons IR
.
???displayArticle.abstract???
Transcripts approximately 14.5 kilobases in length from 14 different genes that encode for dynein heavy chains have been identified in poly(A)+ RNA from sea urchin embryos. Analysis of the changes in level of these dynein transcripts in response to deciliation, together with their sequence relatedness, suggests that 11 or more of these genes encode dynein isoforms that participate in regeneration of external cilia on the embryo, whereas the single gene whose deduced sequence closely resembles that of cytoplasmic dynein in other organisms appears not to be involved in this regeneration. The four consensus motifs for phosphate binding found previously in the beta heavy chain of sea urchin dynein are present in all five additional isoforms for which extended sequences have been obtained, suggesting that these sites play a significant role in dynein function. Sequence analysis of a approximately 400 amino acid region encompassing the putative hydrolytic ATP-binding site shows that the dynein genes fall into at least six distinct classes. Most of these classes in sea urchin have a high degree of sequence identity with one of the dynein heavy chain genes identified in Drosophila, indicating that the radiation of the dynein gene family into the present classes occurred at an early stage in the evolution of eukaryotes. Evolutionary changes in cytoplasmic dynein have been more constrained than those in the axonemal dyneins.
Britten,
The single-copy DNA sequence polymorphism of the sea urchin Strongylocentrotus purpuratus.
1978, Pubmed,
Echinobase
Britten,
The single-copy DNA sequence polymorphism of the sea urchin Strongylocentrotus purpuratus.
1978,
Pubmed
,
Echinobase
Cheney,
Phylogenetic analysis of the myosin superfamily.
1993,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Cleveland,
Molecular biology and genetics of tubulin.
1985,
Pubmed
Corthésy-Theulaz,
Cytoplasmic dynein participates in the centrosomal localization of the Golgi complex.
1992,
Pubmed
Dentler,
Microtubule-membrane interactions in cilia. II. Photochemical cross-linking of bridge structures and the identification of a membrane-associated dynein-like ATPase.
1980,
Pubmed
Endow,
A multimember kinesin gene family in Drosophila.
1991,
Pubmed
Endow,
Genetic approaches to molecular motors.
1992,
Pubmed
Eshel,
Cytoplasmic dynein is required for normal nuclear segregation in yeast.
1993,
Pubmed
Felsenstein,
Phylogenies from molecular sequences: inference and reliability.
1988,
Pubmed
Foltz,
Ionic strength-dependent isoforms of sea urchin egg dynein.
1988,
Pubmed
,
Echinobase
Fregien,
Molecular cloning of five individual stage- and tissue-specific mRNA sequences from sea urchin pluteus embryos.
1983,
Pubmed
,
Echinobase
Gibbons,
A cytoplasmic dynein heavy chain in sea urchin embryos.
1992,
Pubmed
,
Echinobase
Gibbons,
Multiple nucleotide-binding sites in the sequence of dynein beta heavy chain.
1991,
Pubmed
,
Echinobase
Gibbons,
Cilia and flagella of eukaryotes.
1981,
Pubmed
Gibbons,
Vanadate-sensitized cleavage of dynein heavy chains by 365-nm irradiation of demembranated sperm flagella and its effect on the flagellar motility.
1987,
Pubmed
,
Echinobase
Gibbons,
Dynein ATPases as microtubule motors.
1988,
Pubmed
Gibbons,
Photosensitized cleavage of dynein heavy chains. Cleavage at the "V1 site" by irradiation at 365 nm in the presence of ATP and vanadate.
1987,
Pubmed
,
Echinobase
Gibbons,
A PCR procedure to determine the sequence of large polypeptides by rapid walking through a cDNA library.
1991,
Pubmed
,
Echinobase
Goldstein,
The kinesin superfamily: tails of functional redundancy.
1991,
Pubmed
Gong,
Stimulation of tubulin gene transcription by deciliation of sea urchin embryos.
1987,
Pubmed
,
Echinobase
Goodenough,
High-pressure liquid chromatography fractionation of Chlamydomonas dynein extracts and characterization of inner-arm dynein subunits.
1987,
Pubmed
Goodson,
Molecular evolution of the myosin family: relationships derived from comparisons of amino acid sequences.
1993,
Pubmed
Grissom,
Two distinct isoforms of sea urchin egg dynein.
1992,
Pubmed
,
Echinobase
Harlow,
Coordinate and selective beta-tubulin gene expression associated with cilium formation in sea urchin embryos.
1987,
Pubmed
,
Echinobase
Higgins,
CLUSTAL V: improved software for multiple sequence alignment.
1992,
Pubmed
King,
Characterization of monoclonal antibodies against Chlamydomonas flagellar dyneins by high-resolution protein blotting.
1985,
Pubmed
,
Echinobase
King,
Localization of an intermediate chain of outer arm dynein by immunoelectron microscopy.
1990,
Pubmed
King,
Structure of the alpha and beta heavy chains of the outer arm dynein from Chlamydomonas flagella. Location of epitopes and protease-sensitive sites.
1988,
Pubmed
Koonce,
Dynein from Dictyostelium: primary structure comparisons between a cytoplasmic motor enzyme and flagellar dynein.
1992,
Pubmed
,
Echinobase
Koonce,
Identification and immunolocalization of cytoplasmic dynein in Dictyostelium.
1990,
Pubmed
Lefebvre,
Regulation of the synthesis and assembly of ciliary and flagellar proteins during regeneration.
1986,
Pubmed
Lye,
Identification of a microtubule-based cytoplasmic motor in the nematode C. elegans.
1987,
Pubmed
Mikami,
Molecular cloning of the retrograde transport motor cytoplasmic dynein (MAP 1C).
1993,
Pubmed
,
Echinobase
Mocz,
ATP-insensitive interaction of the amino-terminal region of the beta heavy chain of dynein with microtubules.
1993,
Pubmed
,
Echinobase
Neely,
HMW-2, the Sertoli cell cytoplasmic dynein from rat testis, is a dimer composed of nearly identical subunits.
1990,
Pubmed
Ogawa,
Four ATP-binding sites in the midregion of the beta heavy chain of dynein.
1991,
Pubmed
,
Echinobase
Pallini,
Cytoplasmic nucleoside-triphosphatase similar to axonemal dynein occur widely in different cell types.
1983,
Pubmed
Paschal,
MAP 1C is a microtubule-activated ATPase which translocates microtubules in vitro and has dynein-like properties.
1987,
Pubmed
Pfarr,
Cytoplasmic dynein is localized to kinetochores during mitosis.
1990,
Pubmed
Piperno,
Monoclonal antibodies to dynein subunits reveal the existence of cytoplasmic antigens in sea urchin egg.
1984,
Pubmed
,
Echinobase
Piperno,
Three distinct inner dynein arms in Chlamydomonas flagella: molecular composition and location in the axoneme.
1990,
Pubmed
Porter,
Dynein isoforms in sea urchin eggs.
1988,
Pubmed
,
Echinobase
Porter,
Dynein structure and function.
1989,
Pubmed
Puissant,
An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1990,
Pubmed
Rasmusson,
A family of dynein genes in Drosophila melanogaster.
1994,
Pubmed
,
Echinobase
Sale,
Isolated beta-heavy chain subunit of dynein translocates microtubules in vitro.
1988,
Pubmed
,
Echinobase
Sale,
The substructure of isolated and in situ outer dynein arms of sea urchin sperm flagella.
1985,
Pubmed
,
Echinobase
Saraste,
The P-loop--a common motif in ATP- and GTP-binding proteins.
1990,
Pubmed
Schroeder,
Vesicle transport along microtubular ribbons and isolation of cytoplasmic dynein from Paramecium.
1990,
Pubmed
Shpetner,
Characterization of the microtubule-activated ATPase of brain cytoplasmic dynein (MAP 1C).
1988,
Pubmed
Simmen,
Length and sequence polymorphisms in the ribosomal gene spacer of the Hawaiian sea urchin, T. gratilla.
1986,
Pubmed
,
Echinobase
Stephens,
Differential protein synthesis and utilization during cilia formation in sea urchin embryos.
1977,
Pubmed
,
Echinobase
Stephens,
Dynein from serotonin-activated cilia and flagella: extraction characteristics and distinct sites for cAMP-dependent protein phosphorylation.
1992,
Pubmed
Steuer,
Localization of cytoplasmic dynein to mitotic spindles and kinetochores.
1990,
Pubmed
,
Echinobase
Stewart,
Identification and partial characterization of six members of the kinesin superfamily in Drosophila.
1991,
Pubmed
Vale,
Rotation and translocation of microtubules in vitro induced by dyneins from Tetrahymena cilia.
1988,
Pubmed
Walker,
Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
1982,
Pubmed
Witman,
Axonemal dyneins.
1992,
Pubmed