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RNA
2013 Jun 01;196:852-60. doi: 10.1261/rna.039131.113.
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Identification of purple sea urchin telomerase RNA using a next-generation sequencing based approach.
Li Y
,
Podlevsky JD
,
Marz M
,
Qi X
,
Hoffmann S
,
Stadler PF
,
Chen JJ
.
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Telomerase is a ribonucleoprotein (RNP) enzyme essential for telomere maintenance and chromosome stability. While the catalytic telomerase reverse transcriptase (TERT) protein is well conserved across eukaryotes, telomerase RNA (TR) is extensively divergent in size, sequence, and structure. This diversity prohibits TR identification from many important organisms. Here we report a novel approach for TR discovery that combines in vitro TR enrichment from total RNA, next-generation sequencing, and a computational screening pipeline. With this approach, we have successfully identified TR from Strongylocentrotus purpuratus (purple sea urchin) from the phylum Echinodermata. Reconstitution of activity in vitro confirmed that this RNA is an integral component of sea urchin telomerase. Comparative phylogenetic analysis against vertebrate TR sequences revealed that the purple sea urchin TR contains vertebrate-like template-pseudoknot and H/ACA domains. While lacking a vertebrate-like CR4/5 domain, sea urchin TR has a unique central domain critical for telomerase activity. This is the first TR identified from the previously unexplored invertebrate clade and provides the first glimpse of TR evolution in the deuterostome lineage. Moreover, our TR discovery approach is a significant step toward the comprehensive understanding of telomerase RNP evolution.
Altschul,
Basic local alignment search tool.
1990, Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Blackburn,
Telomerase: an RNP enzyme synthesizes DNA.
2011,
Pubmed
Bley,
RNA-protein binding interface in the telomerase ribonucleoprotein.
2011,
Pubmed
Britten,
The single-copy DNA sequence polymorphism of the sea urchin Strongylocentrotus purpuratus.
1978,
Pubmed
,
Echinobase
Chen,
Secondary structure of vertebrate telomerase RNA.
2000,
Pubmed
Chen,
Template boundary definition in mammalian telomerase.
2003,
Pubmed
Cifuentes-Rojas,
Two RNA subunits and POT1a are components of Arabidopsis telomerase.
2011,
Pubmed
Dandjinou,
A phylogenetically based secondary structure for the yeast telomerase RNA.
2004,
Pubmed
Gardner,
Rfam: updates to the RNA families database.
2009,
Pubmed
Greider,
A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis.
1989,
Pubmed
Gunisova,
Identification and comparative analysis of telomerase RNAs from Candida species reveal conservation of functional elements.
2009,
Pubmed
Hoffmann,
Fast mapping of short sequences with mismatches, insertions and deletions using index structures.
2009,
Pubmed
Hsu,
Telomerase core components protect Candida telomeres from aberrant overhang accumulation.
2007,
Pubmed
Jády,
Human telomerase RNA and box H/ACA scaRNAs share a common Cajal body-specific localization signal.
2004,
Pubmed
Kachouri-Lafond,
Large telomerase RNA, telomere length heterogeneity and escape from senescence in Candida glabrata.
2009,
Pubmed
Keefe,
One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag.
2001,
Pubmed
Leonardi,
TER1, the RNA subunit of fission yeast telomerase.
2008,
Pubmed
Lowe,
tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.
1997,
Pubmed
Mason,
Telomerase structure function.
2011,
Pubmed
McEachern,
Runaway telomere elongation caused by telomerase RNA gene mutations.
1995,
Pubmed
Podlevsky,
It all comes together at the ends: telomerase structure, function, and biogenesis.
2012,
Pubmed
Podlevsky,
The telomerase database.
2008,
Pubmed
Qi,
The common ancestral core of vertebrate and fungal telomerase RNAs.
2013,
Pubmed
Seto,
Saccharomyces cerevisiae telomerase is an Sm small nuclear ribonucleoprotein particle.
1999,
Pubmed
Sinclair,
Characterization of the telomere regions of scleractinian coral, Acropora surculosa.
2007,
Pubmed
,
Echinobase
Sodergren,
The genome of the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Tzfati,
Template boundary in a yeast telomerase specified by RNA structure.
2000,
Pubmed
Webb,
Identification and characterization of the Schizosaccharomyces pombe TER1 telomerase RNA.
2008,
Pubmed
Xie,
Structure and function of the smallest vertebrate telomerase RNA from teleost fish.
2008,
Pubmed
Zuker,
Mfold web server for nucleic acid folding and hybridization prediction.
2003,
Pubmed
