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Echinobase
ECB-ART-32904
J Cell Biol 1985 Jun 01;1006:1875-83. doi: 10.1083/jcb.100.6.1875.
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Modulation of the asymmetry of sea urchin sperm flagellar bending by calmodulin.

Brokaw CJ , Nagayama SM .


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Sea urchin spermatozoa demembranated with Triton X-100 in the presence of EGTA, termed potentially asymmetric, generate asymmetric bending waves in reactivation solutions containing EGTA. After they are converted to the potentially symmetric condition by extraction with Triton and millimolar Ca++, they generate symmetric bending waves in reactivation solutions containing EGTA. In the presence of EGTA, their asymmetry can be restored by addition of brain calmodulin or the concentrated supernatant obtained from extraction with Triton and millimolar Ca++. These extracts contain calmodulin, as assayed by gel electrophoresis, radioimmunoassay, activation of brain phosphodiesterase, and Ca++-dependent binding of asymmetry-restoring activity to a trifluorophenothiazine-affinity resin. Conversion to the potentially symmetric condition can also be achieved with trifluoperazine substituted for Triton during the exposure to millimolar Ca++, which suggests that the calmodulin-binding activity of Triton is important for this conversion. These observations suggest that the conversion to the potentially symmetric condition is the result of removal of some of the axonemal calmodulin and provide additional evidence for axonemal calmodulin as a mediator of the effect of Ca++ on the asymmetry of flagellar bending.

???displayArticle.pubmedLink??? 3922994
???displayArticle.pmcLink??? PMC2113586
???displayArticle.link??? J Cell Biol
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Genes referenced: dnah3 LOC100887844

References [+] :
Andreasen, Purification of a novel calmodulin binding protein from bovine cerebral cortex membranes. 1983, Pubmed