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ECB-ART-31102
Proc Natl Acad Sci U S A 1992 Nov 15;8922:10568-72. doi: 10.1073/pnas.89.22.10568.
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SpCOUP-TF: a sea urchin member of the steroid/thyroid hormone receptor family.

Chan SM , Xu N , Niemeyer CC , Bone JR , Flytzanis CN .


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Embryonic nuclear proteins from the sea urchin Strongylocentrotus purpuratus bind in vitro to a cis-acting element that lies upstream of the actin gene CyIIIb and consists of two direct repeats homologous to steroid hormone response elements. This sea urchin element is specifically recognized by transcription factor COUP-TF from HeLa cell nuclear extracts as well. A sea urchin gene homologous to the human COUP-TF1 gene was detected by blot hybridization to S. purpuratus genomic DNA. Screening of a genomic DNA library with a human COUP-TF1 cDNA probe produced overlapping genomic clones that carry the S. purpuratus gene. Our results indicate that the sea urchin homologue has a structure similar to the human COUP-TF1 gene, i.e., conserved intron positions characteristic of this subgroup of steroid hormone receptors. Sequencing of the exons that encode the DNA- and ligand-binding domains of the sea urchin protein revealed 96% and 92% amino acid identity to the domains of the human protein, respectively. Transcripts derived from the S. purpuratus COUP-TF homologous gene were detected in ovarian and embryonic RNAs from various stages.

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Genes referenced: cyiiib LOC100887844 LOC115919910 LOC590297 potekp1 thrb

References [+] :
Bagchi, Purification and characterization of chicken ovalbumin gene upstream promoter transcription factor from homologous oviduct cells. 1987, Pubmed