ECB-ART-35566
Hoppe Seylers Z Physiol Chem
1980 Oct 01;36110:1511-6. doi: 10.1515/bchm2.1980.361.2.1511.
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Identification of the sialic acids from the egg jelly coat of the sea urchin Pseudocentrotus depressus (Okayama).
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The jelly coat substance of the sea urchin eggs of Pseudocentrotus depressus (Okayama) was found to contain 0.35% sialic acids. After isolation of the sialic acids from the purified sulfated sialoglycoprotein containing 11% of sialic acids, analysis by colorimetry, thin-layer chromatography and gas-liquid chromatography/mass spectrometry demonstrated the nature of the sialic acids to be N-glycolylneuraminic acid and 9-O-acetyl-N-glycoloylneuraminic acid. The molar ratio between both sialic acids was found to be 1 : 1. Since it is known that O-acyl groups are partially released during the hydrolysis and isolation procedure, it can be assumed that in the native glycoprotein the percentage of O-acetylation is much higher than 50%. The sugar and sulfate composition of the glycoprotein is also described.
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Genes referenced: LOC100887844