Castellano I et al. (2018), Biotic and environmental stress induces nitrati...

ECB-ART-46188

Sci Rep 2018 Mar 15;81:4610. doi: 10.1038/s41598-018-22861-1.

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Biotic and environmental stress induces nitration and changes in structure and function of the sea urchin major yolk protein toposome.

Castellano I , Migliaccio O , Ferraro G , Maffioli E , Marasco D , Merlino A , Zingone A , Tedeschi G , Palumbo A .

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The major yolk protein toposome plays crucial roles during gametogenesis and development of sea urchins. We previously found that nitration of toposome increases in the gonads of a Paracentrotus lividus population living in a marine protected area affected by toxic blooms of Ostreospsis cf. ovata, compared to control populations. This modification is associated with ovatoxin accumulation, high levels of nitric oxide in the gonads, and a remarkable impairment of progeny development. However, nothing is known about the environmental-mediated-regulation of the structure and biological function of toposome. Here, we characterize through wide-ranging biochemical and structural analyses the nitrated toposome of sea urchins exposed to the bloom, and subsequently detoxified. The increased number of nitrated tyrosines in toposome of sea urchins collected during algal bloom induced structural changes and improvement of the Ca2+-binding affinity of the protein. After 3 months'' detoxification, ovatoxin was undetectable, and the number of nitric oxide-modified tyrosines was reduced. However, the nitration of specific residues was irreversible and occurred also in embryos treated with metals, used as a proxy of environmental pollutants. The structural and functional changes of toposome caused by nitration under adverse environmental conditions may be related to the defective development of sea urchins'' progeny.

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Genes referenced: LOC100887844 LOC583082 LOC590297

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	Figure 3. Scheme of nitrated residues of toposome. Sequence coverage and nitrated residues of toposome of sea urchins collected prior to the bloom (C), at the bloom phase (T0), and after stabulation in sea water for 56 (T1) and 92 days (T2). The figure shows the sequence coverage of toposome and the nitrated residues indicated as a black line.
	Figure 4. Structural properties and Ca2+ affinity of toposome. Far UV CD and intrinsic fluorescence spectra of toposome purified from control and T0 animals. CD spectra (panel a,b) measured in 10 mM Tris-HCl buffer at pH = 7.8 and 10 °C. Spectra of the proteins (0.1 mg mL−1) were compared with that obtained for a sample upon treatment with EDTA and after addition of 1 mM Ca2+. Intrinsic fluorescence spectra (panel c,d) measured in 10 mM Tris-HCl buffer at pH = 7.8 and 20 °C. Spectra of the proteins (0.05 mg mL−1) were compared with those obtained for the protein upon addition of increasing concentration of Ca2+ (protein to Ca2+ molar ratio between 1:0.3 and 1:20).
	Figure 5. Ca2+ binding properties of toposome. ITC Calorimetric raw and integrated data for C (20 µM) titration with CaCl2 (2 mM). Data fitting was achieved with ‘one set of sites’ model.
	Figure 6. Pattern of nitrated proteins in sea urchin embryos after metals treatment. (a) Early blastula stage. (b) Swimming blastula stage. 1 = Control in sea water, 2 = Mn2+ 1.8 × 10−5 M, 3 = Mn2+ 3.6 × 10−5 M, 4 = Mn2+ 7.8 × 10−5 M, 5 = Mn2+ 15.5 × 10−5 M, 6 = Mn2+ 31.2 × 10−5 M, 7 = Cd2+ 5 × 10−7 M, 8 = Cd2+ 10−6 M, 9 = 5.2 × 10−6 M. Representative experiment showing the western blot analysis with anti-nitrotyrosine (anti-NT) antibodies. Anti-actin is used as loading control.
	Figure 7. Scheme of nitrated residues of toposome after metals treatment. Nitrated residues are indicated as a black line. In red the residues found nitrated also in samples collected at bloom (T0) or after stabulation (T1, T2) in sea water (see Fig. 3 for the comparison).