Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Dev Biol
2018 Mar 15;4352:138-149. doi: 10.1016/j.ydbio.2017.12.015.
Show Gene links
Show Anatomy links
Identification of neural transcription factors required for the differentiation of three neuronal subtypes in the sea urchin embryo.
Slota LA
,
McClay DR
.
???displayArticle.abstract???
Correct patterning of the nervous system is essential for an organism''s survival and complex behavior. Embryologists have used the sea urchin as a model for decades, but our understanding of sea urchin nervous system patterning is incomplete. Previous histochemical studies identified multiple neurotransmitters in the pluteus larvae of several sea urchin species. However, little is known about how, where and when neural subtypes are differentially specified during development. Here, we examine the molecular mechanisms of neuronal subtype specification in 3 distinct neural subtypes in the Lytechinus variegatus larva. We show that these subtypes are specified through Delta/Notch signaling and identify a different transcription factor required for the development of each neural subtype. Our results show achaete-scute and neurogenin are proneural for the serotonergic neurons of the apical organ and cholinergic neurons of the ciliary band, respectively. We also show that orthopedia is not proneural but is necessary for the differentiation of the cholinergic/catecholaminergic postoral neurons. Interestingly, these transcription factors are used similarly during vertebrate neurogenesis. We believe this study is a starting point for building a neural gene regulatory network in the sea urchin and for finding conserved deuterostome neurogenic mechanisms.
Adams,
Rapid adaptation to food availability by a dopamine-mediated morphogenetic response.
2011, Pubmed,
Echinobase
Adams,
Rapid adaptation to food availability by a dopamine-mediated morphogenetic response.
2011,
Pubmed
,
Echinobase
Angerer,
The evolution of nervous system patterning: insights from sea urchin development.
2011,
Pubmed
,
Echinobase
Artavanis-Tsakonas,
Notch signaling: cell fate control and signal integration in development.
1999,
Pubmed
Bertrand,
Proneural genes and the specification of neural cell types.
2002,
Pubmed
Blaschke,
Widespread programmed cell death in proliferative and postmitotic regions of the fetal cerebral cortex.
1996,
Pubmed
Burke,
Sea urchin neural development and the metazoan paradigm of neurogenesis.
2014,
Pubmed
,
Echinobase
Burke,
A genomic view of the sea urchin nervous system.
2006,
Pubmed
,
Echinobase
Burke,
The structure of the nervous system of the pluteus larva of Strongylocentrotus purpuratus.
1978,
Pubmed
,
Echinobase
Bush,
Biparous: a novel bHLH gene expressed in neuronal and glial precursors in Drosophila.
1996,
Pubmed
Castro,
Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif.
2006,
Pubmed
Cavalieri,
Impairing Otp homeodomain function in oral ectoderm cells affects skeletogenesis in sea urchin embryos.
2003,
Pubmed
,
Echinobase
Davidson,
Emerging properties of animal gene regulatory networks.
2010,
Pubmed
Di Bernardo,
Spatially restricted expression of PlOtp, a Paracentrotus lividus orthopedia-related homeobox gene, is correlated with oral ectodermal patterning and skeletal morphogenesis in late-cleavage sea urchin embryos.
1999,
Pubmed
,
Echinobase
Doonan,
HLH-3 is a C. elegans Achaete/Scute protein required for differentiation of the hermaphrodite-specific motor neurons.
2008,
Pubmed
Fernandes,
Orthopedia transcription factor otpa and otpb paralogous genes function during dopaminergic and neuroendocrine cell specification in larval zebrafish.
2013,
Pubmed
Frank,
HLH-14 is a C. elegans achaete-scute protein that promotes neurogenesis through asymmetric cell division.
2003,
Pubmed
Garner,
Neurogenesis in sea urchin embryos and the diversity of deuterostome neurogenic mechanisms.
2016,
Pubmed
,
Echinobase
Ghysen,
From DNA to form: the achaete-scute complex.
1988,
Pubmed
Gramates,
FlyBase at 25: looking to the future.
2017,
Pubmed
Hartenstein,
The evolution of early neurogenesis.
2015,
Pubmed
Hinman,
Developmental gene regulatory network evolution: insights from comparative studies in echinoderms.
2014,
Pubmed
,
Echinobase
Huang,
Proneural bHLH genes in development and disease.
2014,
Pubmed
Kageyama,
Dynamic regulation of Notch signaling in neural progenitor cells.
2009,
Pubmed
Katow,
Immunohistochemical and ultrastructural properties of the larval ciliary band-associated strand in the sea urchin Hemicentrotus pulcherrimus.
2016,
Pubmed
,
Echinobase
Longabaugh,
Computational representation of developmental genetic regulatory networks.
2005,
Pubmed
Lu,
BMP and Delta/Notch signaling control the development of amphioxus epidermal sensory neurons: insights into the evolution of the peripheral sensory system.
2012,
Pubmed
Ma,
neurogenin1 is essential for the determination of neuronal precursors for proximal cranial sensory ganglia.
1998,
Pubmed
Ma,
Identification of neurogenin, a vertebrate neuronal determination gene.
1996,
Pubmed
Ma,
Neurogenin1 and neurogenin2 control two distinct waves of neurogenesis in developing dorsal root ganglia.
1999,
Pubmed
Marlow,
Larval body patterning and apical organs are conserved in animal evolution.
2014,
Pubmed
McClay,
Evolutionary crossroads in developmental biology: sea urchins.
2011,
Pubmed
,
Echinobase
Mellott,
Notch signaling patterns neurogenic ectoderm and regulates the asymmetric division of neural progenitors in sea urchin embryos.
2017,
Pubmed
,
Echinobase
Meyer,
Neurogenesis in an annelid: characterization of brain neural precursors in the polychaete Capitella sp. I.
2009,
Pubmed
Mummery-Widmer,
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
2009,
Pubmed
Oliveri,
Gene regulatory network controlling embryonic specification in the sea urchin.
2004,
Pubmed
,
Echinobase
Range,
Specification and positioning of the anterior neuroectoderm in deuterostome embryos.
2014,
Pubmed
,
Echinobase
Roybon,
GABAergic differentiation induced by Mash1 is compromised by the bHLH proteins Neurogenin2, NeuroD1, and NeuroD2.
2010,
Pubmed
Ryu,
Orthopedia homeodomain protein is essential for diencephalic dopaminergic neuron development.
2007,
Pubmed
Simeone,
Orthopedia, a novel homeobox-containing gene expressed in the developing CNS of both mouse and Drosophila.
1994,
Pubmed
Simionato,
atonal- and achaete-scute-related genes in the annelid Platynereis dumerilii: insights into the evolution of neural basic-Helix-Loop-Helix genes.
2008,
Pubmed
Sinigaglia,
The bilaterian head patterning gene six3/6 controls aboral domain development in a cnidarian.
2013,
Pubmed
Skeath,
The achaete-scute complex: generation of cellular pattern and fate within the Drosophila nervous system.
1994,
Pubmed
Sommer,
neurogenins, a novel family of atonal-related bHLH transcription factors, are putative mammalian neuronal determination genes that reveal progenitor cell heterogeneity in the developing CNS and PNS.
1996,
Pubmed
Sommer,
Neural stem cells and regulation of cell number.
2002,
Pubmed
Stolfi,
Migratory neuronal progenitors arise from the neural plate borders in tunicates.
2015,
Pubmed
Strathmann,
Time and extent of ciliary response to particles in a non-filtering feeding mechanism.
2007,
Pubmed
,
Echinobase
Sur,
Spatiotemporal regulation of nervous system development in the annelid Capitella teleta.
2017,
Pubmed
Sutherby,
Histamine is a modulator of metamorphic competence in Strongylocentrotus purpuratus (Echinodermata: Echinoidea).
2012,
Pubmed
,
Echinobase
Tessmar-Raible,
Conserved sensory-neurosecretory cell types in annelid and fish forebrain: insights into hypothalamus evolution.
2007,
Pubmed
Vervoort,
The evolution of the neural basic Helix-Loop-Helix proteins.
2001,
Pubmed
Wei,
The sea urchin animal pole domain is a Six3-dependent neurogenic patterning center.
2009,
Pubmed
,
Echinobase
Wei,
Neurogenic gene regulatory pathways in the sea urchin embryo.
2016,
Pubmed
,
Echinobase
Wei,
Direct development of neurons within foregut endoderm of sea urchin embryos.
2011,
Pubmed
,
Echinobase
Yaguchi,
Expression of tryptophan 5-hydroxylase gene during sea urchin neurogenesis and role of serotonergic nervous system in larval behavior.
2003,
Pubmed
,
Echinobase
Yaguchi,
Zinc finger homeobox is required for the differentiation of serotonergic neurons in the sea urchin embryo.
2012,
Pubmed
,
Echinobase
Yankura,
Gene regulatory network for neurogenesis in a sea star embryo connects broad neural specification and localized patterning.
2013,
Pubmed
,
Echinobase
Yuan,
The Drosophila neurogenin Tap functionally interacts with the Wnt-PCP pathway to regulate neuronal extension and guidance.
2016,
Pubmed
