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Microorganisms
2022 Jan 19;102:. doi: 10.3390/microorganisms10020207.
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Significant Changes in Bacterial Communities Associated with Pocillopora Corals Ingestion by Crown-of-Thorns Starfish: An Important Factor Affecting the Coral's Health.
Qin Z
,
Yu K
,
Chen S
,
Chen B
,
Yao Q
,
Yu X
,
Pan N
,
Wei X
.
Abstract
Coral ingestion by crown-of-thorns starfish (COTS) is an important cause of coral reef degradation, although the impacts of COTS feeding on coral-associated microbial communities are not well understood. Therefore, in this study, we analyzed the coral tissue-weight, Symbiodiniaceae density (SD), bacterial community composition, and the predicted functions of bacterial genes associated with Pocillopora corals in healthy portions and feeding scars, following COTS feeding. Coral tissue-weight loss rate in the feeding scars was 71.3-94.95%. The SDs were significantly lower in the feeding scars, and the SD-loss rate was 92.05% ± 2.12%. The relative abundances of bacterial communities associated with Pocillopora corals after COTS feeding changed significantly and were almost completely reorganized at the phylum and genus levels. Analysis of the microbial metagenomic-functional capacities showed that numerous physiological functions of the coral-bacterial holobionts in the feeding scars were different, including amino acid metabolism, xenobiotic biodegradation and metabolism, lipid metabolism, membrane transport, signal transduction, and cell motility, and all these capacities could be corroborated based on metagenomic, transcriptomic or proteomic technologies. Overall, our research suggests that coral holobionts may be destroyed by COTS, and our findings imply that bacterial communities in feeding scars could affect the health of Pocillopora corals.
42030502 National Natural Science Foundation of China, 42090041 National Natural Science Foundation of China, AD17129063 Guangxi scientific projects, AA17204074 Guangxi scientific projects, 2014BGXZGX03 Bagui Fellowship from Guangxi Province of China
Figure 1. Coral-symbiotic Symbiodiniaceae density (SD) of Pocillopora corals between healthy portions and feeding scars ingested by crown-of-thorns starfish in the central South China Sea (SCS).
Figure 2. Relative abundances of coral-associated microbial community members in Pocillopora sampled from healthy portions and feeding scars at the phylum level, as determined using the RDP classifier.
Figure 3. Heatmap showing the percentage of the 40 most abundant microbial genera associated with Pocillopora sampled from healthy portions and feeding scars.
Figure 4. Student’s t-test results showing differences in bacterial abundance at the species level in Pocillopora, sampled from healthy portions and feeding scars. ** 0.001 < p < 0.01; *** p < 0.001.
Figure 5. Principal coordinate analysis (PCoA) based on operational taxonomic units (OTUs) with grouping based on 60% similarity, as determined by complete linkage-cluster analysis. PC1 and PC2 explained 43.82% and 9.21% of the total variation, respectively.
Figure 6. Mean relative abundance of each predicted functional trait in the KEGG pathways (level 2) using PICRUSt (V1.0.0) to analyze the predicted metagenomes, based on the 16S rRNA gene-sequencing data of coral-associated bacteria in Pocillopora sampled from healthy portions and feeding scars. The error bars indicate the standard deviation. ** 0.001 < p < 0.01; *** p < 0.001.
Bellwood,
Confronting the coral reef crisis.
2004, Pubmed
Bellwood,
Confronting the coral reef crisis.
2004,
Pubmed
Ben-Haim,
Temperature-regulated bleaching and lysis of the coral Pocillopora damicornis by the novel pathogen Vibrio coralliilyticus.
2003,
Pubmed
Bolger,
Trimmomatic: a flexible trimmer for Illumina sequence data.
2014,
Pubmed
Bolyen,
Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2.
2019,
Pubmed
Bourne,
Changes in coral-associated microbial communities during a bleaching event.
2008,
Pubmed
Bourne,
Coral reef invertebrate microbiomes correlate with the presence of photosymbionts.
2013,
Pubmed
Bourne,
Insights into the Coral Microbiome: Underpinning the Health and Resilience of Reef Ecosystems.
2016,
Pubmed
Brauer,
Triggering of the stomach eversion reflex of Acanthaster planci by coral extracts.
1970,
Pubmed
,
Echinobase
Chesher,
Destruction of Pacific Corals by the Sea Star Acanthaster planci.
1969,
Pubmed
,
Echinobase
D Ainsworth,
The coral core microbiome identifies rare bacterial taxa as ubiquitous endosymbionts.
2015,
Pubmed
De'ath,
The 27-year decline of coral cover on the Great Barrier Reef and its causes.
2012,
Pubmed
,
Echinobase
Ding,
Genomic Insight into the Host-Endosymbiont Relationship of Endozoicomonas montiporae CL-33(T) with its Coral Host.
2016,
Pubmed
Hakim,
The gut microbiome of the sea urchin, Lytechinus variegatus, from its natural habitat demonstrates selective attributes of microbial taxa and predictive metabolic profiles.
2016,
Pubmed
,
Echinobase
Hughes,
Global warming and recurrent mass bleaching of corals.
2017,
Pubmed
Kayal,
Predator crown-of-thorns starfish (Acanthaster planci) outbreak, mass mortality of corals, and cascading effects on reef fish and benthic communities.
2012,
Pubmed
,
Echinobase
Langille,
Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences.
2013,
Pubmed
Li,
Bacterial dynamics within the mucus, tissue and skeleton of the coral Porites lutea during different seasons.
2014,
Pubmed
Maher,
Multiple stressors interact primarily through antagonism to drive changes in the coral microbiome.
2019,
Pubmed
Mahmoud,
Coral-Associated Actinobacteria: Diversity, Abundance, and Biotechnological Potentials.
2016,
Pubmed
McFall-Ngai,
Animals in a bacterial world, a new imperative for the life sciences.
2013,
Pubmed
Meyer,
Community shifts in the surface microbiomes of the coral Porites astreoides with unusual lesions.
2014,
Pubmed
Mori,
Design and experimental application of a novel non-degenerate universal primer set that amplifies prokaryotic 16S rRNA genes with a low possibility to amplify eukaryotic rRNA genes.
2014,
Pubmed
Neave,
Differential specificity between closely related corals and abundant Endozoicomonas endosymbionts across global scales.
2017,
Pubmed
Ogden,
Grazing by the Echinoid Diadema antillarum Philippi: Formation of Halos around West Indian Patch Reefs.
1973,
Pubmed
,
Echinobase
Peixoto,
Coral Probiotics: Premise, Promise, Prospects.
2021,
Pubmed
Pogoreutz,
Dominance of Endozoicomonas bacteria throughout coral bleaching and mortality suggests structural inflexibility of the Pocillopora verrucosa microbiome.
2018,
Pubmed
Qin,
Microbiome of juvenile corals in the outer reef slope and lagoon of the South China Sea: insight into coral acclimatization to extreme thermal environments.
2021,
Pubmed
Qiu,
Community-level destruction of hard corals by the sea urchin Diadema setosum.
2014,
Pubmed
,
Echinobase
Rice,
Different nitrogen sources speed recovery from corallivory and uniquely alter the microbiome of a reef-building coral.
2019,
Pubmed
Tout,
Increased seawater temperature increases the abundance and alters the structure of natural Vibrio populations associated with the coral Pocillopora damicornis.
2015,
Pubmed
Zhang,
PEAR: a fast and accurate Illumina Paired-End reAd mergeR.
2014,
Pubmed
Ziegler,
Bacterial community dynamics are linked to patterns of coral heat tolerance.
2017,
Pubmed
van Oppen,
Coral microbiome dynamics, functions and design in a changing world.
2019,
Pubmed
van de Water,
Host-microbe interactions in octocoral holobionts - recent advances and perspectives.
2018,
Pubmed
van de Water,
Seasonal Stability in the Microbiomes of Temperate Gorgonians and the Red Coral Corallium rubrum Across the Mediterranean Sea.
2018,
Pubmed