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J Cell Biol
1975 Dec 01;673:501-17. doi: 10.1083/jcb.67.3.501.
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Microtubular origin of mitotic spindle form birefringence. Demonstration of the applicability of Wiener''s equation.
Sato H
,
Ellis GW
,
Inoué S
.
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Meiosis I metaphase spindles were isolated from oocytes of the sea-star Pisaster ochraceus by a method that produced no detectable net loss in spindle birefringence. Some of the spindles were fixed immediately and embedded and sectioned for electron microscopy. Others were laminated between gelatine pellicles in a perfusion chamber, then fixed and sequentially and reversibly imbibed with a series of media of increasing refractive indices. Electron microscopy showed little else besides microtubules in the isolates, and no other component present could account for the observed form birefringence. An Ambronn plot of the birefringent retardation measured during imbibition was a good least squares fit to a computer generated theoretical curve based on the Bragg-Pippard rederivation of the Wiener curve for form birefringence. The data were best fit by the curve for rodlet index (n1) = 1.512, rodlet volume fraction (f) = 0.0206, and coefficient of intrinsic birefringence = 4.7 X 10(-5). The value obtained for n1 is unequivocal and is virtually as good as the refractometer determinations of imbibing medium index on which it is based. The optically interactive volume of the microtubule subunit, calculated from our electron microscope determination of spindle microtubule distribution (106/mum2), 13 protofilaments per microtubules, an 8 nm repeat distance and our best value for f, is compatible with known subunit dimensions as determined by other means. We also report curves fitted to the results of Ambronn imbibition of Bouin''s-fixed Lytechinus spindles and to the Noll and Weber muscle imbibition data.
Allen,
Pattern of birefringence in the giant amoeba, Chaos carolinensis.
1972, Pubmed
Allen,
Pattern of birefringence in the giant amoeba, Chaos carolinensis.
1972,
Pubmed
Behnke,
Some aspects of microtubules in spermatocyte meiosis in a crane fly (Nephrotoma suturalis Loew): intranuclear and intrachromosomal microtubules.
1966,
Pubmed
Bendet,
Birefringence of spermatozoa. II. Form birefringence of bull sperm.
1972,
Pubmed
Bryan,
The isolation of the meiosis in spindle from the mature oocyte of Pisaster ochraceus.
1970,
Pubmed
,
Echinobase
Cassim,
Birefringence of muscle proteins and the problem of structural birefringence.
1968,
Pubmed
Cassim,
Intrinsic birefringence of poly-gamma-benzyl-L-glutamate, a helical polypeptide, and the theory of birefringence.
1965,
Pubmed
Cohen,
X-ray diffraction from microtubules.
1971,
Pubmed
Cohen,
X-ray patterns from microtubules.
1975,
Pubmed
Colby,
Intrinsic birefringence of glycerinated myofibrils.
1971,
Pubmed
Erickson,
Microtubule surface lattice and subunit structure and observations on reassembly.
1974,
Pubmed
Forer,
The concentrations of dry matter in mitotic apparatuses in vivo and after isolation from sea-urchin zygotes.
1972,
Pubmed
,
Echinobase
Goldman,
The structure and some properties of the isolated mitotic apparatus.
1969,
Pubmed
Grimstone,
Observations on the substructure of flagellar fibres.
1966,
Pubmed
INOUE,
Studies on depolarization of light at microscope lens surfaces. II. The simultaneous realization of high resolution and high sensitivity with the polarizing microscope.
1957,
Pubmed
Inoué,
Functional organization of mitotic microtubules. Physical chemistry of the in vivo equilibrium system.
1975,
Pubmed
,
Echinobase
Inoué,
Growth and lability of Chaetopterus oocyte mitotic spindles isolated in the presence of porcine brain tubulin.
1974,
Pubmed
Inoué,
Cell motility by labile association of molecules. The nature of mitotic spindle fibers and their role in chromosome movement.
1967,
Pubmed
KANE,
THE MITOTIC APPARATUS. PHYSICAL-CHEMICAL FACTORS CONTROLLING STABILITY.
1965,
Pubmed
,
Echinobase
KANE,
The mitotic apparatus: isolation by controlled pH.
1962,
Pubmed
Kanatani,
Isolation and indentification on meiosis inducing substance in starfish Asterias amurensis.
1969,
Pubmed
,
Echinobase
Kane,
The mitotic apparatus. Structural changes after isolation.
1965,
Pubmed
,
Echinobase
LaFountain,
Birefringence and fine structure of spindles in spermatocytes of Nephrotoma suturalis at metaphase of first meiotic division.
1974,
Pubmed
Olmsted,
Microtubules.
1973,
Pubmed
PAGE,
FILAMENT LENGTHS IN STRIATED MUSCLE.
1963,
Pubmed
Rebhun,
Reversible restoration of the birefringence of cold-treated, isolated mitotic apparatus of surf clam eggs with chick brain tubulin.
1974,
Pubmed
Rebhun,
Ultrastructure and birefringence of the isolated mitotic apparatus of marine eggs.
1967,
Pubmed
,
Echinobase
TAYLOR,
Birefringence of protein solutions and biological systems. I.
1963,
Pubmed
Taylor,
The contractile basis of amoeboid movement. I. The chemical control of motility in isolated cytoplasm.
1973,
Pubmed
Tilney,
Microtubules: evidence for 13 protofilaments.
1973,
Pubmed
,
Echinobase
Weisenberg,
In vitro polymerization of microtubules into asters and spindles in homogenates of surf clam eggs.
1975,
Pubmed
Weisenberg,
Microtubule formation in vitro in solutions containing low calcium concentrations.
1972,
Pubmed
