ECB-ART-46877
J Extracell Vesicles
2018 Jan 01;71:1535750. doi: 10.1080/20013078.2018.1535750.
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Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines.
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Witwer KW
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Aikawa E
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Alcaraz MJ
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Anderson JD
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Andriantsitohaina R
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Antoniou A
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Arab T
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Archer F
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Atkin-Smith GK
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Ayre DC
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Bach JM
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Bachurski D
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Baharvand H
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Balaj L
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Baldacchino S
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Bauer NN
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Beckham C
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Bedina Zavec A
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Benmoussa A
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Berardi AC
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Boilard E
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Bongiovanni A
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Breakefield X
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Breglio AM
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Brennan MÁ
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Brigstock DR
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Brisson A
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Broekman ML
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Bromberg JF
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Bryl-Górecka P
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Buch S
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Buck AH
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Burger D
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Busatto S
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Buschmann D
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Bussolati B
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Buzás EI
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Byrd JB
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Camussi G
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Carter DR
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Caruso S
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Chamley LW
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Chang YT
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Chen C
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Chen S
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Cheng L
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Chin AR
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Clayton A
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Clerici SP
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Cocks A
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Cocucci E
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Coffey RJ
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Cordeiro-da-Silva A
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Couch Y
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Coumans FA
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Coyle B
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Crescitelli R
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Criado MF
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D'Souza-Schorey C
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Das S
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Datta Chaudhuri A
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de Candia P
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De Santana EF
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De Wever O
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Del Portillo HA
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Demaret T
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Deville S
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Devitt A
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Dhondt B
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Di Vizio D
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Dieterich LC
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Dolo V
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Dominguez Rubio AP
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Dominici M
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Dourado MR
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Driedonks TA
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Duarte FV
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Duncan HM
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Eichenberger RM
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Ekström K
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El Andaloussi S
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Elie-Caille C
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Erdbrügger U
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Falcón-Pérez JM
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Fatima F
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Fish JE
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Flores-Bellver M
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Försönits A
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Frelet-Barrand A
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Fricke F
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Fuhrmann G
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Gabrielsson S
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Gärtner K
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Gaudin R
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Gho YS
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Giebel B
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Gilbert C
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Gimona M
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Giusti I
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Goberdhan DC
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Görgens A
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Gorski SM
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Greening DW
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Gross JC
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Gualerzi A
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Gupta GN
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Gustafson D
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Handberg A
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Haraszti RA
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Harrison P
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Hegyesi H
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Hendrix A
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Hill AF
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Hochberg FH
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Hoffmann KF
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Holder B
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Holthofer H
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Hosseinkhani B
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Hu G
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Huang Y
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Huber V
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Hunt S
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Ibrahim AG
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Ikezu T
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Inal JM
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Isin M
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Ivanova A
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Jackson HK
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Jacobsen S
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Jay SM
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Jayachandran M
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Jenster G
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Jiang L
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Johnson SM
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Jones JC
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Jong A
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Jovanovic-Talisman T
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Jung S
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Kalluri R
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Kano SI
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Kaur S
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Kawamura Y
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Keller ET
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Khamari D
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Khomyakova E
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Khvorova A
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Kierulf P
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Kim KP
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Kislinger T
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Kosanović MM
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Kovács ÁF
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Krämer-Albers EM
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Krasemann S
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Krause M
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Kurochkin IV
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Kusuma GD
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Kuypers S
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Laitinen S
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Langevin SM
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Languino LR
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Lannigan J
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Lässer C
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Laurent LC
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Lavieu G
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Lázaro-Ibáñez E
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Le Lay S
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Lee MS
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Lee YXF
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Lemos DS
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Lenassi M
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Leszczynska A
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Li IT
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Liao K
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Libregts SF
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Ligeti E
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Lim R
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Lim SK
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Linē A
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Linnemannstöns K
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Llorente A
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Lombard CA
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Lorenowicz MJ
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Lörincz ÁM
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Lötvall J
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Lovett J
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Lowry MC
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Loyer X
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Lunavat TR
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Maas SL
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Malhi H
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Marcilla A
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Mariani J
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Mariscal J
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Martens-Uzunova ES
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Martin-Jaular L
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Martinez MC
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Martins VR
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Mathieu M
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Mathivanan S
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Maugeri M
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McGinnis LK
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McVey MJ
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Meckes DG
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Meehan KL
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Mertens I
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Minciacchi VR
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Morales-Kastresana A
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Morhayim J
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Mullier F
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Muraca M
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Musante L
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Muth DC
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Myburgh KH
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Najrana T
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Nawaz M
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Nazarenko I
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Nejsum P
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Neri C
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Neri T
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Østergaard O
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Park J
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Pegtel DM
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Peinado H
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Perut F
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Pfaffl MW
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Phinney DG
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Pieters BC
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Pink RC
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Pisetsky DS
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Pogge von Strandmann E
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Polakovicova I
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Poon IK
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Ricklefs FL
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Shetty AK
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Siljander PR
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Skowronek A
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Snyder OL
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Tomasini R
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Torrecilhas AC
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Tosar JP
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Toxavidis V
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Urbanelli L
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van Balkom BW
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van der Grein SG
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van Herwijnen MJ
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van Royen ME
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van Wijnen AJ
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Vechetti IJ
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Verweij FJ
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Vestad B
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Viñas JL
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Visnovitz T
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Vukman KV
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Wahlgren J
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Watson DC
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Wauben MH
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Weaver A
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Webber JP
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Weber V
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Wehman AM
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Weiss DJ
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Welsh JA
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Wendt S
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Wheelock AM
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Wiener Z
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Witte L
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Wolfram J
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Xagorari A
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Xander P
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Xu J
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Yan X
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Yáñez-Mó M
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Yin H
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Yuana Y
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Zappulli V
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Zarubova J
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Žėkas V
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Zhang JY
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Zhao Z
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Zheng L
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Zheutlin AR
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Zickler AM
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Zimmermann P
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Zivkovic AM
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Zocco D
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Zuba-Surma EK
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Abstract
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
PubMed ID: 30637094
PMC ID: PMC6322352
Article link: J Extracell Vesicles
Grant support: [+]
R01 CA204621 NCI NIH HHS , P01 CA140043 NCI NIH HHS , P01 CA069246 NCI NIH HHS , R01 CA193473 NCI NIH HHS , R21 MH118164 NIMH NIH HHS, R01 DA047807 NIDA NIH HHS , R56 AG057430 NIA NIH HHS , BB/E002080/1 Biotechnology and Biological Sciences Research Council , R01 CA224769 NCI NIH HHS , U01 CA230697 NCI NIH HHS , R01 DA040385 NIDA NIH HHS , R01 DK111378 NIDDK NIH HHS , T32 OD011089 NIH HHS , R42 NS065515 NINDS NIH HHS , U01 HL126499 NHLBI NIH HHS , R01 GM117916 NIGMS NIH HHS , P30 DK058404 NIDDK NIH HHS , R33 MH118164 NIMH NIH HHS, U19 CA179514 NCI NIH HHS , P30 CA008748 NCI NIH HHS , UL1 TR001860 NCATS NIH HHS , T32 AG052375 NIA NIH HHS , KL2 TR001859 NCATS NIH HHS , T32 HL086350 NHLBI NIH HHS , MR/R023166/1 Medical Research Council , R01 CA218526 NCI NIH HHS , 19076 Cancer Research UK, R01 MH113645 NIMH NIH HHS
Genes referenced: LOC115919910
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