Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Re-evaluation of the structure and physiological function of guanidino kinases in fruitfly (Drosophila), sea urchin (Psammechinus miliaris) and man.
Wyss M
,
Maughan D
,
Wallimann T
.
???displayArticle.abstract???
Purification and biophysical characterization of mitochondrial creatine kinase (Mi-CK) from sperm of the sea urchin Psammechinus miliaris, as well as gel-permeation chromatography of human heart Mi-CK demonstrate that these two Mi-CK isoenzymes form highly symmetrical octameric molecules with an M(r) of approx. 350,000, a value similar to that found for all other Mi-CK isoenzymes investigated so far. The absolute evolutionary conservation of this oligomeric form from sea urchins to mammals points both to its essentiality for Mi-CK function and to an important role of octameric Mi-CK in the energy metabolism of tissues and cells with high and fluctuating energy demands. To investigate whether a similar physiological principle also operates in an even more distantly related animal phylum, the arginine kinase (ArgK) isoenzyme system of Drosophila flight muscle was investigated with two independent subcellular fractionation procedures and subsequent analysis of the fractions by SDS/PAGE, immunoblotting and native isoenzyme electrophoresis. In contrast with a previous report [Munneke and Collier (1988) Biochem. Genet. 26, 131-141], strong evidence against the occurrence of a Mi-ArgK isoenzyme in Drosophila was obtained. The findings of the present study are discussed in the context of CK and ArgK function in general and of structural and bioenergetic differences between vertebrate striated muscles and arthropod flight muscles.
Bessman,
Transport of energy in muscle: the phosphorylcreatine shuttle.
1981, Pubmed
Bessman,
Transport of energy in muscle: the phosphorylcreatine shuttle.
1981,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Brdiczka,
Contact sites between mitochondrial envelope membranes. Structure and function in energy- and protein-transfer.
1991,
Pubmed
Brdiczka,
The importance of the outer mitochondrial compartment in regulation of energy metabolism.
1994,
Pubmed
Clarke,
The size and detergent binding of membrane proteins.
1975,
Pubmed
Grace,
Purification and characterization of human mitochondrial creatine kinase. A single enzyme form.
1983,
Pubmed
Ivy,
Skeletal muscle determinants of maximum aerobic power in man.
1980,
Pubmed
Kottke,
Location and regulation of octameric mitochondrial creatine kinase in the contact sites.
1991,
Pubmed
Lang,
Localization of arginine kinase in muscles fibres of Drosophila melanogaster.
1980,
Pubmed
Maughan,
Protein diffusivities in skinned frog skeletal muscle fibers.
1988,
Pubmed
Maughan,
On the organization and diffusion of glycolytic enzymes in skeletal muscle.
1989,
Pubmed
Munneke,
Cytoplasmic and mitochondrial arginine kinases in Drosophila: evidence for a single gene.
1988,
Pubmed
Mühlebach,
Sequence homology and structure predictions of the creatine kinase isoenzymes.
1994,
Pubmed
Peña,
The beta subunit of the Drosophila melanogaster ATP synthase: cDNA cloning, amino acid analysis and identification of the protein in adult flies.
1993,
Pubmed
Quest,
Membrane association of flagellar creatine kinase in the sperm phosphocreatine shuttle.
1991,
Pubmed
,
Echinobase
Quest,
Myristoylation of flagellar creatine kinase in the sperm phosphocreatine shuttle is linked to its membrane association properties.
1992,
Pubmed
,
Echinobase
Rojo,
Mitochondrial creatine kinase mediates contact formation between mitochondrial membranes.
1991,
Pubmed
Schlegel,
Native mitochondrial creatine kinase forms octameric structures. I. Isolation of two interconvertible mitochondrial creatine kinase forms, dimeric and octameric mitochondrial creatine kinase: characterization, localization, and structure-function relationships.
1988,
Pubmed
Schlegel,
Mitochondrial creatine kinase from cardiac muscle and brain are two distinct isoenzymes but both form octameric molecules.
1988,
Pubmed
Schlegel,
Functional studies with the octameric and dimeric form of mitochondrial creatine kinase. Differential pH-dependent association of the two oligomeric forms with the inner mitochondrial membrane.
1990,
Pubmed
Schnyder,
Structure of the mitochondrial creatine kinase octamer: high-resolution shadowing and image averaging of single molecules and formation of linear filaments under specific staining conditions.
1991,
Pubmed
Schnyder,
Native mitochondrial creatine kinase forms octameric structures. II. Characterization of dimers and octamers by ultracentrifugation, direct mass measurements by scanning transmission electron microscopy, and image analysis of single mitochondrial creatine kinase octamers.
1988,
Pubmed
Schwerzmann,
Oxidative capacity of muscle and mitochondria: correlation of physiological, biochemical, and morphometric characteristics.
1989,
Pubmed
Shields,
Skeletal muscle function and structure after depletion of creatine.
1975,
Pubmed
Shifrin,
Influence of glycerol and other polyhydric alcohol on the quaternary structure of an oligometric protein.
1975,
Pubmed
Soldati,
Alternative ribosomal initiation gives rise to chicken brain-type creatine kinase isoproteins with heterogeneous amino termini.
1990,
Pubmed
Stein,
Macro creatine kinase: determination and differentiation of two types by their activation energies.
1982,
Pubmed
Tombes,
Metabolite channeling: a phosphorylcreatine shuttle to mediate high energy phosphate transport between sperm mitochondrion and tail.
1985,
Pubmed
,
Echinobase
Tombes,
Enzyme termini of a phosphocreatine shuttle. Purification and characterization of two creatine kinase isozymes from sea urchin sperm.
1987,
Pubmed
,
Echinobase
Wallimann,
Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands: the 'phosphocreatine circuit' for cellular energy homeostasis.
1992,
Pubmed
Warmke,
Myosin light chain-2 mutation affects flight, wing beat frequency, and indirect flight muscle contraction kinetics in Drosophila.
1992,
Pubmed
Wegmann,
In situ compartmentation of creatine kinase in intact sarcomeric muscle: the acto-myosin overlap zone as a molecular sieve.
1992,
Pubmed
Wevers,
Mitochondrial creatine kinase (EC 2.7.3.2) in the brain.
1982,
Pubmed
Wigglesworth,
The supply of oxygen to the flight muscles of insects: a theory of tracheole physiology.
1982,
Pubmed
Wothe,
The phosphocreatine shuttle of sea urchin sperm: flagellar creatine kinase resulted from a gene triplication.
1990,
Pubmed
,
Echinobase
Wyss,
Mitochondrial creatine kinase: a key enzyme of aerobic energy metabolism.
1992,
Pubmed
Wyss,
Metabolite channelling in aerobic energy metabolism.
1992,
Pubmed
Wyss,
Mitochondrial creatine kinase from chicken brain. Purification, biophysical characterization, and generation of heterodimeric and heterooctameric molecules with subunits of other creatine kinase isoenzymes.
1990,
Pubmed
