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Kazazoglou T
,
Schackmann RW
,
Fosset M
,
Shapiro BM
.
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As a prerequisite to fertilization, sea urchin sperm undergo an acrosome reaction that is mediated in part by increased permeability to Ca2+, with an attendant rapid, massive intracellular Ca2+ accumulation. The acrosome reaction is inhibited by Ca2+ channel antagonists, including verapamil, D600, and dihydropyridines such as nitrendipine, nimodipine, and nisoldipine. To examine the interaction of Ca2+ antagonists with sperm, a plasma membrane preparation enriched for Na+,K+-ATPase was isolated from sea urchin sperm. These plasma membranes specifically bound [3H]nitrendipine and [3H]verapamil at concentrations similar to those that inhibit the acrosome reaction. The binding of verapamil was sigmoidal and half-maximal at 1 microM. There was a high specificity in the binding interaction, since by competition binding verapamil, (-)-D600, and (+)-D600 had different relative Kd values, 11, 2.5, and 0.5 microM, respectively. These data suggest that sperm mediate the Ca2+ influx required for induction of the acrosome reaction via Ca2+ channels with properties similar, but not identical, to those of other excitable tissues.
APPEL,
[(N-methyl-N-homoveratryl)-gamma-aminopropyl] -3,4-dimethoxyphenylacetonitrile, its detection in biological materials and its behavior in blood].
1962, Pubmed
APPEL,
[(N-methyl-N-homoveratryl)-gamma-aminopropyl] -3,4-dimethoxyphenylacetonitrile, its detection in biological materials and its behavior in blood].
1962,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Cantino,
Changes in subcellular elemental distributions accompanying the acrosome reaction in sea urchin sperm.
1983,
Pubmed
,
Echinobase
Christen,
Interactions between sperm and sea urchin egg jelly.
1983,
Pubmed
,
Echinobase
Collins,
The role of calcium ions in the acrosome reaction of sea urchin sperm: regulation of exocytosis.
1977,
Pubmed
,
Echinobase
Cross,
Isolation and electrophoretic characterization of the plasma membrane of sea-urchin sperm.
1983,
Pubmed
,
Echinobase
Curtis,
Solubilization of the calcium antagonist receptor from rat brain.
1983,
Pubmed
DePover,
[3H]nimodipine specific binding to cardiac myocytes and subcellular fractions.
1983,
Pubmed
Decker,
A study of factors involved in induction of the acrosomal reaction in sperm of the sea urchin, Arbacia punctulata.
1976,
Pubmed
,
Echinobase
Ehlert,
The interaction of [3H]nitrendipine with receptors for calcium antagonists in the cerebral cortex and heart of rats.
1982,
Pubmed
Fosset,
[3H]nitrendipine labelling of the Ca2+ channel in skeletal muscle.
1982,
Pubmed
Fosset,
[3H]nitrendipine receptors in skeletal muscle.
1983,
Pubmed
Gache,
(Na+, K+)-activated adenosinetriphosphatase of axonal membranes, cooperativity and control. Steady-state analysis.
1976,
Pubmed
Galizzi,
[3H] verapamil binding sites in skeletal muscle transverse tubule membranes.
1984,
Pubmed
Garbers,
The elevation of cyclic AMP concentrations in flagella-less sea urchin sperm heads.
1981,
Pubmed
,
Echinobase
Gould,
[3H]nitrendipine-labeled calcium channels discriminate inorganic calcium agonists and antagonists.
1982,
Pubmed
Grover,
Subcellular distribution of [3H]nitrendipine binding in smooth muscle.
1984,
Pubmed
Hagiwara,
Calcium channel.
1981,
Pubmed
Kazazoglou,
Ontogenic appearance of Ca2+ channels characterized as binding sites for nitrendipine during development of nervous, skeletal and cardiac muscle systems in the rat.
1983,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Langer,
Heart: excitation-contraction coupling.
1973,
Pubmed
Mason,
Cytochrome c oxidase from bakers' yeast. I. Isolation and properties.
1973,
Pubmed
Renaud,
Differentiation of receptor sites for [3H]nitrendipine in chick hearts and physiological relation to the slow Ca2+ channel and to excitation-contraction coupling.
1984,
Pubmed
Schackmann,
A partial sequence of ionic changes associated with the acrosome reaction of Strongylocentrotus purpuratus.
1981,
Pubmed
,
Echinobase
Schackmann,
Membrane potential depolarization and increased intracellular pH accompany the acrosome reaction of sea urchin sperm.
1981,
Pubmed
,
Echinobase
Schackmann,
The acrosome reaction of Strongylocentrotus purpuratus sperm. Ion requirements and movements.
1978,
Pubmed
,
Echinobase
Shapiro,
Molecular approaches to the study of fertilization.
1981,
Pubmed
Talbot,
The role of calcium in the acrosome reaction: an analysis using ionophore A23187.
1976,
Pubmed
,
Echinobase
Tsien,
Calcium channels in excitable cell membranes.
1983,
Pubmed
Williams,
Specific binding of the calcium antagonist [3H]nitrendipine to subcellular fractions isolated from canine myocardium. Evidence for high affinity binding to ryanodine-sensitive sarcoplasmic reticulum vesicles.
1983,
Pubmed
