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ECB-ART-47961
Stain Technol 1975 Sep 01;505:351-4. doi: 10.3109/10520297509117086.
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A decalification method for ultrastructure of echinoderm tissues.

Dietrich HF , Fontaine AR .


Abstract
Decalcification of echinoderm tissues for electron microscopy can be achieved after glutaraldehyde-osmium fixation by treatment with a 1:1 mixture of 2% ascorbic acid and 0.3 M NaCl for 12-24 hours. Electron photomicrographs of material decalcified by this procedure are superior to those from EDTA-treated tissue and show few of the deleteriour effects produced by EDTA.

PubMed ID: 813335
Article link: Stain Technol