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Echinobase
ECB-ART-31867
Mol Cell Biol 1989 Mar 01;93:1120-7. doi: 10.1128/mcb.9.3.1120-1127.1989.
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cys-3, the positive-acting sulfur regulatory gene of Neurospora crassa, encodes a protein with a putative leucine zipper DNA-binding element.

Fu YH , Paietta JV , Mannix DG , Marzluf GA .


Abstract
The sulfur-regulatory circuit of Neurospora crassa consists of a set of unlinked structural genes which encode sulfur-catabolic enzymes and two major regulatory genes which govern their expression. The positive-acting cys-3 regulatory gene is required to turn on the expression of the sulfur-related enzymes, whereas the other regulatory gene, scon, acts in a negative fashion to repress the synthesis of the same set of enzymes. Expression of the cys-3 regulatory gene was found to be controlled by scon and by sulfur availability. The nucleotide sequence of the cys-3 gene was determined and can be translated to yield a protein of molecular weight 25,892 which displays significant homology with the oncogene protein Fos, yeast GCN4 protein, and sea urchin histone H1. Moreover, the putative cys-3 protein has a well-defined leucine zipper element plus an adjacent charged region which together may make up a DNA-binding site. A cys-3 mutant and a cys-3 temperature-sensitive mutant lead to substitutions of glutamine for basic amino acids within the charged region and thus may alter DNA-binding properties of the cys-3 protein.

PubMed ID: 2524646
PMC ID: PMC362702
Article link: Mol Cell Biol
Grant support: [+]

Genes referenced: LOC100887844 LOC115918644 LOC579838 LOC583082

References [+] :
Aviv, Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. 1972, Pubmed