Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Front Physiol
2020 Apr 02;11:559348. doi: 10.3389/fphys.2020.559348.
Show Gene links
Show Anatomy links
The Effect of Pedal Peptide-Type Neuropeptide on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus.
Ding K
,
Zhang L
,
Fan X
,
Guo X
,
Liu X
,
Yang H
.
???displayArticle.abstract???
Neuropeptides are endogenous active substances that are present in nervous tissues and participate in behavioral and physiological processes of the animal system. Locomotor behavior is basic to predation, escape, reproduction in animals, and neuropeptides play an important role in locomotion. In this study, the function of pedal peptide-type neuropeptide (PDP) in the process of locomotor behavior of the sea cucumber Apostichopus japonicus was evaluated. The locomotor behavior of A. japonicus was recorded by infrared camera before and after PDP administration, and muscle physiology was studied by ultra performance liquid chromatography and quadrupole time-off-light mass spectrometry (UPLC-Q-TOF-MS) to clarify the potential physiological mechanisms. The results showed that PDP enhanced the cumulative duration of moving significantly at the 7th h after injection, and reduced the mean and maximum velocity by 16.90 and 14.22% in A. japonicus. The data of muscle metabolomics suggested that some significantly changed metabolites were related to locomotor behavior of sea cucumbers. The decreases of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) might result in the increases of lysophosphatidylcholines (lysoPC) and lysophosphatidylethanolamine (lysoPE), and suggested the change of fluidity and permeability in the muscle cell membrane, which would affect the physiology and function of muscle cells, and finally alter the locomotor behavior. In addition, the increased level of arachidonic acid (ARA) might activate K+ ion channels and then affect the signaling of muscle cells, or promote the sensitivity of muscle cells to Ca2+ and then result in the contractility of longitudinal muscles in sea cucumbers. ARA was also involved in the linoleic acid metabolism which was the only pathway that disturbed significantly after PDP administration. In conclusion, PDP participated in the regulation of locomotor behavior in the sea cucumber, and the decreased PE and PC, increased lysoPC, lysoPE and ARA might be the potential physiological mechanisms that responsible for behavioral effects of PDP in A. japonicus.
FIGURE 1. Total distance traveled per hour (A), cumulative duration of movement per hour (B), and mean and maximum velocity (C,D) for A. japonicus in the control (CON) and pedal peptide-type neuropeptide injected (PDP) groups. Each symbol or bar and vertical line represents the mean ± SEM (N = 12, p < 0.05).
FIGURE 2. Average total number of steps taken per hour (A), stride (B), stride frequency (C), and stride velocity (D) of A. japonicus in the control (CON) and pedal peptide-type neuropeptide injected (PDP) groups. Each vertical line represents the mean ± SEM (N = 12, p < 0.05).
FIGURE 3. The PLS-DA (A) and OPLS-DA (B) scores plot of muscle metabolites from the control (CON) and pedal peptide-type neuropeptide injected (PDP) groups. The abscissa and ordinate represent the first principal component (PC1) and the second principal component (PC2), respectively.
FIGURE 4. The heat maps of overall differential metabolites from the control (CON) and pedal peptide-type neuropeptide injected (PDP) groups. Each line represents a differential metabolite and each cross represents a muscle sample. Different colors represent different higher abundance intensity (mean value acquired from all detected samples of the same group).
FIGURE 5. The correlation analysis of overall differential metabolites from the control (CON) and pedal peptide-type neuropeptide injected (PDP) groups. The color of each line represents the Pearson correlation coefficient of two differential metabolites. Red for positive correlation and blue for negative correlation.
FIGURE 6. The KEGG pathway enrichment of overall differential metabolites from the control (CON) and pedal peptide-type neuropeptide injected (PDP) groups. Horizontal axis for enriched pathway; vertical axis for the significance level of pathway enrichment. Above the red and blue dashed lines represents p < 0.01 and p < 0.05.
Chen,
The Neuropeptides FLP-2 and PDF-1 Act in Concert To Arouse Caenorhabditis elegans Locomotion.
2016, Pubmed
Chen,
The Neuropeptides FLP-2 and PDF-1 Act in Concert To Arouse Caenorhabditis elegans Locomotion.
2016,
Pubmed
Chen,
Neuropeptide precursors and neuropeptides in the sea cucumber Apostichopus japonicus: a genomic, transcriptomic and proteomic analysis.
2019,
Pubmed
,
Echinobase
Ding,
The Effect of Melatonin on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus.
2019,
Pubmed
,
Echinobase
Florin,
Orphan neuropeptide NocII, a putative pronociceptin maturation product, stimulates locomotion in mice.
1997,
Pubmed
Funai,
Skeletal Muscle Phospholipid Metabolism Regulates Insulin Sensitivity and Contractile Function.
2016,
Pubmed
Funai,
Muscle lipogenesis balances insulin sensitivity and strength through calcium signaling.
2013,
Pubmed
Gong,
Arachidonic acid inhibits myosin light chain phosphatase and sensitizes smooth muscle to calcium.
1992,
Pubmed
Goodyear,
Exercise, glucose transport, and insulin sensitivity.
1998,
Pubmed
Górski,
Effect of endurance training on the phospholipid content of skeletal muscles in the rat.
1999,
Pubmed
Hall,
Involvement of pedal peptide in locomotion in Aplysia: modulation of foot muscle contractions.
1990,
Pubmed
He,
Regulation of circadian locomotor rhythm by neuropeptide Y-like system in Drosophila melanogaster.
2013,
Pubmed
Heden,
Looking Beyond Structure: Membrane Phospholipids of Skeletal Muscle Mitochondria.
2016,
Pubmed
Hofer,
Evidence for a role of orcokinin-related peptides in the circadian clock controlling locomotor activity of the cockroach Leucophaea maderae.
2006,
Pubmed
Hou,
The neuropeptide F/nitric oxide pathway is essential for shaping locomotor plasticity underlying locust phase transition.
2017,
Pubmed
Hu,
Activation of K+ channel in vascular smooth muscles by cytochrome P450 metabolites of arachidonic acid.
1993,
Pubmed
Ji,
Responses of Mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics.
2013,
Pubmed
Jékely,
Global view of the evolution and diversity of metazoan neuropeptide signaling.
2013,
Pubmed
Kahsai,
Neuropeptides in the Drosophila central complex in modulation of locomotor behavior.
2010,
Pubmed
Kato,
Neuronal peptides induce oocyte maturation and gamete spawning of sea cucumber, Apostichopus japonicus.
2009,
Pubmed
,
Echinobase
Kim,
Identification of a novel starfish neuropeptide that acts as a muscle relaxant.
2016,
Pubmed
,
Echinobase
Lee,
Author Correction: Skeletal muscle phosphatidylcholine and phosphatidylethanolamine respond to exercise and influence insulin sensitivity in men.
2018,
Pubmed
Lin,
Pedal peptide/orcokinin-type neuropeptide signaling in a deuterostome: The anatomy and pharmacology of starfish myorelaxant peptide in Asterias rubens.
2017,
Pubmed
,
Echinobase
Lin,
Functional characterization of a second pedal peptide/orcokinin-type neuropeptide signaling system in the starfish Asterias rubens.
2018,
Pubmed
,
Echinobase
Lloyd,
Sequence of pedal peptide: a novel neuropeptide from the central nervous system of Aplysia.
1989,
Pubmed
Longley,
Neuronal control of pedal sole cilia in the pond snail Lymnaea stagnalis appressa.
2013,
Pubmed
Luo,
Simultaneous determination of multiple intracellular metabolites in glycolysis, pentose phosphate pathway and tricarboxylic acid cycle by liquid chromatography-mass spectrometry.
2007,
Pubmed
Mitchell,
Effect of dietary arachidonic acid supplementation on acute muscle adaptive responses to resistance exercise in trained men: a randomized controlled trial.
2018,
Pubmed
Nadiv,
Elevated protein tyrosine phosphatase activity and increased membrane viscosity are associated with impaired activation of the insulin receptor kinase in old rats.
1994,
Pubmed
Nicholson,
'Metabonomics': understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data.
1999,
Pubmed
O'Connor,
High-throughput bioanalysis with simultaneous acquisition of metabolic route data using ultra performance liquid chromatography coupled with time-of-flight mass spectrometry.
2006,
Pubmed
Onchoi,
High performance liquid chromatography for determination of urinary metabolites of toluene, xylene and styrene and its application.
2008,
Pubmed
Ordway,
Arachidonic acid and other fatty acids directly activate potassium channels in smooth muscle cells.
1989,
Pubmed
Oresic,
Dysregulation of lipid and amino acid metabolism precedes islet autoimmunity in children who later progress to type 1 diabetes.
2008,
Pubmed
Pan,
Influence of flow velocity on motor behavior of sea cucumber Apostichopus japonicus.
2015,
Pubmed
,
Echinobase
Pauls,
Drosophila carboxypeptidase D (SILVER) is a key enzyme in neuropeptide processing required to maintain locomotor activity levels and survival rate.
2019,
Pubmed
Pañeda,
Neuropeptide S reinstates cocaine-seeking behavior and increases locomotor activity through corticotropin-releasing factor receptor 1 in mice.
2009,
Pubmed
Pilch,
Coordinate modulation of D-glucose transport activity and bilayer fluidity in plasma membranes derived from control and insulin-treated adipocytes.
1980,
Pubmed
Pinillos,
Food intake inhibition by melatonin in goldfish (Carassius auratus).
2001,
Pubmed
Plumb,
Use of liquid chromatography/time-of-flight mass spectrometry and multivariate statistical analysis shows promise for the detection of drug metabolites in biological fluids.
2003,
Pubmed
Robertson,
Metabonomics: evaluation of nuclear magnetic resonance (NMR) and pattern recognition technology for rapid in vivo screening of liver and kidney toxicants.
2000,
Pubmed
Rowe,
Neuropeptides and polypeptide hormones in echinoderms: new insights from analysis of the transcriptome of the sea cucumber Apostichopus japonicus.
2014,
Pubmed
,
Echinobase
Rowe,
The neuropeptide transcriptome of a model echinoderm, the sea urchin Strongylocentrotus purpuratus.
2012,
Pubmed
,
Echinobase
Saha,
GPI-anchored protein organization and dynamics at the cell surface.
2016,
Pubmed
Selathurai,
The CDP-Ethanolamine Pathway Regulates Skeletal Muscle Diacylglycerol Content and Mitochondrial Biogenesis without Altering Insulin Sensitivity.
2015,
Pubmed
Soehler,
Circadian pacemaker coupling by multi-peptidergic neurons in the cockroach Leucophaea maderae.
2011,
Pubmed
Stangier,
Orcokinin: a novel myotropic peptide from the nervous system of the crayfish, Orconectes limosus.
1992,
Pubmed
Stuart,
Bed-rest-induced insulin resistance occurs primarily in muscle.
1988,
Pubmed
Sun,
Metabolic responses to intestine regeneration in sea cucumbers Apostichopus japonicus.
2017,
Pubmed
,
Echinobase
Takagi,
Lipid composition of sarcoplasmic reticulum of human skeletal muscle.
1971,
Pubmed
Wei,
Light affects the branching pattern of peptidergic circadian pacemaker neurons in the brain of the cockroach Leucophaea maderae.
2011,
Pubmed
Wilson,
High resolution "ultra performance" liquid chromatography coupled to oa-TOF mass spectrometry as a tool for differential metabolic pathway profiling in functional genomic studies.
2005,
Pubmed
Wilson,
Obesity, very low density lipoproteins, and glucose intolerance over fourteen years: The Framingham Study.
1981,
Pubmed
Wu,
Metabolomic analysis reveals that carnitines are key regulatory metabolites in phase transition of the locusts.
2012,
Pubmed
Yang,
Aplysia Locomotion: Network and Behavioral Actions of GdFFD, a D-Amino Acid-Containing Neuropeptide.
2016,
Pubmed
